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Overexpression Of Hepatocyte Nuclear Factor-1beta Predicting Poor Prognosis Is Associated With Biliary Phenotype In Patients With Hepatocellular Carcinoma.

Yu DD, Jing YY, Guo SW, Ye F, Lu W, Li Q, Dong YL, Gao L, Yang YT, Yang Y, Wu MC, Wei LX - Sci Rep (2015)

Bottom Line: We found that HNF-1B expression was associated with pathological subtype of primary tumor, and HNF-1B expression in HCC tissue may be associated with the change of phenotype on recurrence.However, no correlation between HNF-1B expression and survival was found in ICC patients.HNF-1B-positive malignant cells could be bipotential cells and give rise to both hepatocytic and cholangiocytic lineages during tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai, China.

ABSTRACT
Hepatocyte nuclear factor-1beta (HNF-1B) is involved in the hepatobiliary specification of hepatoblasts to cholangiocytes during liver development, and is strongly expressed throughout adult biliary epithelium. The aim of this study was to examine the expression of HNF-1B in different pathologic subtypes of primary liver cancer, including hepatocellular carcinoma (HCC) and cholangiocarcinoma (ICC), and the relationship between HNF-1B expression, clinicopathological features and prognosis. We retrospectively investigated 2 cohorts of patients, including 183 HCCs and 69 ICCs. The expression of HNF-1B was examined by immunohistochemistry. We found that HNF-1B expression was associated with pathological subtype of primary tumor, and HNF-1B expression in HCC tissue may be associated with the change of phenotype on recurrence. The HNF-1B expression was positively correlated with biliary/HPC (hepatic progenitor cell) markers expression. Further, multivariable analysis showed that HNF-1B expression was an independent prognostic factor for both overall survival and disease-free survival of HCC patients. However, no correlation between HNF-1B expression and survival was found in ICC patients. In summary, HCC with high HNF-1B expression displayed biliary phenotype and tended to show poorer prognosis. HNF-1B-positive malignant cells could be bipotential cells and give rise to both hepatocytic and cholangiocytic lineages during tumorigenesis.

No MeSH data available.


Related in: MedlinePlus

HNF-1B expression is associated with different pathologic types of primary tumor.(A) HNF-1B immunohistochemical staining patterns in ICC tissues. Representative sections showing HNF-1B expression in 0, 1+, 2+ and 3 + (sacle bar = 100 μm). (B) The differences of HNF-1B expression percentage between HCC patients and ICC patients. (C) The significant differences of HNF-1B expression level between HCC patients and ICC patients (Mean±SD).
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f2: HNF-1B expression is associated with different pathologic types of primary tumor.(A) HNF-1B immunohistochemical staining patterns in ICC tissues. Representative sections showing HNF-1B expression in 0, 1+, 2+ and 3 + (sacle bar = 100 μm). (B) The differences of HNF-1B expression percentage between HCC patients and ICC patients. (C) The significant differences of HNF-1B expression level between HCC patients and ICC patients (Mean±SD).

Mentions: To investigate the relationship between HNF-1B expression and the pathologic type of the primary tumor, we next investigated HNF-1B expression in primary tumor tissues of 183 HCC patients (cohort1) and 69 ICC patients (cohort2) using IHC. Representative images of HNF-1B staining of ICC are illustrated in Fig. 2A. In HNF-1B staining sections of the 183 HCC patients, 82 patients showed HNF1B positive staining, with 35 patients in 3+, 14 patients in 2+, 33 patients in 1+, and 101 patients in 0. Of the 69 ICC patients, 59 patients showed HNF1B positive staining, with 22 patients in 3+, 24 patients in 2+, 13 patients in 1+, and 10 patients in 0 (Fig. 2B). The level of HNF-1B expression was lower in HCC patients than in ICC patients (P < 0.001, Fig. 2C). These results suggested that HNF-1B was associated with different pathologic subtype of primary tumor.


Overexpression Of Hepatocyte Nuclear Factor-1beta Predicting Poor Prognosis Is Associated With Biliary Phenotype In Patients With Hepatocellular Carcinoma.

Yu DD, Jing YY, Guo SW, Ye F, Lu W, Li Q, Dong YL, Gao L, Yang YT, Yang Y, Wu MC, Wei LX - Sci Rep (2015)

HNF-1B expression is associated with different pathologic types of primary tumor.(A) HNF-1B immunohistochemical staining patterns in ICC tissues. Representative sections showing HNF-1B expression in 0, 1+, 2+ and 3 + (sacle bar = 100 μm). (B) The differences of HNF-1B expression percentage between HCC patients and ICC patients. (C) The significant differences of HNF-1B expression level between HCC patients and ICC patients (Mean±SD).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4550878&req=5

f2: HNF-1B expression is associated with different pathologic types of primary tumor.(A) HNF-1B immunohistochemical staining patterns in ICC tissues. Representative sections showing HNF-1B expression in 0, 1+, 2+ and 3 + (sacle bar = 100 μm). (B) The differences of HNF-1B expression percentage between HCC patients and ICC patients. (C) The significant differences of HNF-1B expression level between HCC patients and ICC patients (Mean±SD).
Mentions: To investigate the relationship between HNF-1B expression and the pathologic type of the primary tumor, we next investigated HNF-1B expression in primary tumor tissues of 183 HCC patients (cohort1) and 69 ICC patients (cohort2) using IHC. Representative images of HNF-1B staining of ICC are illustrated in Fig. 2A. In HNF-1B staining sections of the 183 HCC patients, 82 patients showed HNF1B positive staining, with 35 patients in 3+, 14 patients in 2+, 33 patients in 1+, and 101 patients in 0. Of the 69 ICC patients, 59 patients showed HNF1B positive staining, with 22 patients in 3+, 24 patients in 2+, 13 patients in 1+, and 10 patients in 0 (Fig. 2B). The level of HNF-1B expression was lower in HCC patients than in ICC patients (P < 0.001, Fig. 2C). These results suggested that HNF-1B was associated with different pathologic subtype of primary tumor.

Bottom Line: We found that HNF-1B expression was associated with pathological subtype of primary tumor, and HNF-1B expression in HCC tissue may be associated with the change of phenotype on recurrence.However, no correlation between HNF-1B expression and survival was found in ICC patients.HNF-1B-positive malignant cells could be bipotential cells and give rise to both hepatocytic and cholangiocytic lineages during tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai, China.

ABSTRACT
Hepatocyte nuclear factor-1beta (HNF-1B) is involved in the hepatobiliary specification of hepatoblasts to cholangiocytes during liver development, and is strongly expressed throughout adult biliary epithelium. The aim of this study was to examine the expression of HNF-1B in different pathologic subtypes of primary liver cancer, including hepatocellular carcinoma (HCC) and cholangiocarcinoma (ICC), and the relationship between HNF-1B expression, clinicopathological features and prognosis. We retrospectively investigated 2 cohorts of patients, including 183 HCCs and 69 ICCs. The expression of HNF-1B was examined by immunohistochemistry. We found that HNF-1B expression was associated with pathological subtype of primary tumor, and HNF-1B expression in HCC tissue may be associated with the change of phenotype on recurrence. The HNF-1B expression was positively correlated with biliary/HPC (hepatic progenitor cell) markers expression. Further, multivariable analysis showed that HNF-1B expression was an independent prognostic factor for both overall survival and disease-free survival of HCC patients. However, no correlation between HNF-1B expression and survival was found in ICC patients. In summary, HCC with high HNF-1B expression displayed biliary phenotype and tended to show poorer prognosis. HNF-1B-positive malignant cells could be bipotential cells and give rise to both hepatocytic and cholangiocytic lineages during tumorigenesis.

No MeSH data available.


Related in: MedlinePlus