Limits...
Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.

Koch M, Lemke A, Lange C - Stem Cells Int (2015)

Bottom Line: In the grafts T- and B-cell numbers were significantly higher and NK-cells lower in the allo EV kidneys compared to allo.In conclusion, the different cell infiltrates and cytokine transcription suggest distinct immunomodulatory properties of EV in allotransplantation.While the increased T- and B-cells in the allo EV grafts may represent a missing or negative effect on the adaptive immune system, EV seem to influence the innate immune system in a contrary fashion.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery and Transplantation, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

ABSTRACT
Application of mesenchymal stromal cells (MSC) has been proposed for solid organ transplantation based on their potent immunomodulatory effects. Since side effects from the injection of large cells cannot be excluded, the hypothesis rises that extracellular vesicles (EV) may cause immunomodulatory effects comparable to MSC without additional side effects. We used MSC-derived EV in a rat renal transplant model for acute rejection. We analysed peripheral blood leukocytes (PBL), kidney function, graft infiltrating cells, cytokines in the graft, and alloantibody development in animals without (allo) and with EV application (allo EV). There was no difference in kidney function and in the PBL subpopulation including Tregs between allo and allo EV. In the grafts T- and B-cell numbers were significantly higher and NK-cells lower in the allo EV kidneys compared to allo. TNF-α transcription was lower in allo EV grafts compared to allo; there was no difference regarding IL-10 and in the development of alloantibodies. In conclusion, the different cell infiltrates and cytokine transcription suggest distinct immunomodulatory properties of EV in allotransplantation. While the increased T- and B-cells in the allo EV grafts may represent a missing or negative effect on the adaptive immune system, EV seem to influence the innate immune system in a contrary fashion.

No MeSH data available.


Related in: MedlinePlus

Serum creatinine (SCr) at day 7 after transplantation. There was no significant difference between the allo and the allo EV group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4550760&req=5

fig1: Serum creatinine (SCr) at day 7 after transplantation. There was no significant difference between the allo and the allo EV group.

Mentions: In both allo groups we observed a severely impaired kidney function. SCr in the allo groups on day 7 was not different in animals with and without EV application. The mean SCr in the allo group was 3.6 ± 0.7 g/dL and was 3.8 ± 1.0 g/dL (p = 0.63) in the allo EV group (Figure 1).


Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.

Koch M, Lemke A, Lange C - Stem Cells Int (2015)

Serum creatinine (SCr) at day 7 after transplantation. There was no significant difference between the allo and the allo EV group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4550760&req=5

fig1: Serum creatinine (SCr) at day 7 after transplantation. There was no significant difference between the allo and the allo EV group.
Mentions: In both allo groups we observed a severely impaired kidney function. SCr in the allo groups on day 7 was not different in animals with and without EV application. The mean SCr in the allo group was 3.6 ± 0.7 g/dL and was 3.8 ± 1.0 g/dL (p = 0.63) in the allo EV group (Figure 1).

Bottom Line: In the grafts T- and B-cell numbers were significantly higher and NK-cells lower in the allo EV kidneys compared to allo.In conclusion, the different cell infiltrates and cytokine transcription suggest distinct immunomodulatory properties of EV in allotransplantation.While the increased T- and B-cells in the allo EV grafts may represent a missing or negative effect on the adaptive immune system, EV seem to influence the innate immune system in a contrary fashion.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery and Transplantation, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

ABSTRACT
Application of mesenchymal stromal cells (MSC) has been proposed for solid organ transplantation based on their potent immunomodulatory effects. Since side effects from the injection of large cells cannot be excluded, the hypothesis rises that extracellular vesicles (EV) may cause immunomodulatory effects comparable to MSC without additional side effects. We used MSC-derived EV in a rat renal transplant model for acute rejection. We analysed peripheral blood leukocytes (PBL), kidney function, graft infiltrating cells, cytokines in the graft, and alloantibody development in animals without (allo) and with EV application (allo EV). There was no difference in kidney function and in the PBL subpopulation including Tregs between allo and allo EV. In the grafts T- and B-cell numbers were significantly higher and NK-cells lower in the allo EV kidneys compared to allo. TNF-α transcription was lower in allo EV grafts compared to allo; there was no difference regarding IL-10 and in the development of alloantibodies. In conclusion, the different cell infiltrates and cytokine transcription suggest distinct immunomodulatory properties of EV in allotransplantation. While the increased T- and B-cells in the allo EV grafts may represent a missing or negative effect on the adaptive immune system, EV seem to influence the innate immune system in a contrary fashion.

No MeSH data available.


Related in: MedlinePlus