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Haloalkaliphilic Streptomyces spp. AJ8 isolated from solar salt works and its' pharmacological potential.

Jenifer JS, Donio MB, Michaelbabu M, Vincent SG, Citarasu T - AMB Express (2015)

Bottom Line: In vitro antagonistic activity results revealed that, Streptomyces spp.The genomic level identification revealed that, the strain was confirmed as Streptomyces spp.The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari District, 629502, Tamilnadu, India, adlinjenifer86@gmail.com.

ABSTRACT
Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The antimicrobial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, antifungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabolite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.

No MeSH data available.


Related in: MedlinePlus

a FASTA format of NRPS protein query sequence. b Secondary Structure Prediction of the NRPS protein of Streptomyces spp AJ8. (Color indicationsH Helix; S Strands and C Coil). c The predicted 3D model and the estimated global and local accuracy of the NRPS protein. d The structure alignment of NRPS between the first I-TASSER model and the top 5 most similar structure templates in PDB. e The predicted ligand-binding sites of the NRPS protein. (Fluorescent green yellow colour indicated the predicted ligand binding site)
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Fig4: a FASTA format of NRPS protein query sequence. b Secondary Structure Prediction of the NRPS protein of Streptomyces spp AJ8. (Color indicationsH Helix; S Strands and C Coil). c The predicted 3D model and the estimated global and local accuracy of the NRPS protein. d The structure alignment of NRPS between the first I-TASSER model and the top 5 most similar structure templates in PDB. e The predicted ligand-binding sites of the NRPS protein. (Fluorescent green yellow colour indicated the predicted ligand binding site)

Mentions: The Fig. 4a, b shows the protein sequence and the predicted secondary structure of NRPS by I-TASSER analysis. The top five models predicted territory structure for NRPS protein by I-TASSER and the C-score were −4.03, −4.25, −3.36, −4095 and −3.36 respectively (Fig. 4c). The estimated TM-score and RMSD observed of 0.28 ± 0.09 and 15.8 ± 3.2 Å respectively. The TM-align structural alignment results revealed that, the top five PDB hits were 4nl6A, 1qonA, 2c3mB, 2fj0A and 1n35A and its TM scores were 0.843, 0.442, 0.432, 0.426 and 0.426 respectively. The five top most aligned proteins among the NRPS were spliceosome, acetylcholinesterase, ferredoxin oxidoreductases (PFOR), esterase and reovirus polymerase etc. (Fig. 4d). The multiple binding site ligands from different PDB hits (1zeiF, 2xmbA, 4ekdA, 3bz1H and 2hi8X) were confirmed as M-Cresol, Beta-l-Fucose, Cobalt (2+), Chlorophyll a and Bromide etc. (Fig. 4e; Table 4). The predicted EC numbers of the PDB hits weregiven in the Table 5. Based on the results revealed that, the five top most PDB enzyme hits were dipeptidyl carboxypeptidase Dcp, mouse acetylcholinesterase, Cys-418 thiylradical, human acetylcholinesterase and recombinant human butyrylcholinesterase.Fig. 4


Haloalkaliphilic Streptomyces spp. AJ8 isolated from solar salt works and its' pharmacological potential.

Jenifer JS, Donio MB, Michaelbabu M, Vincent SG, Citarasu T - AMB Express (2015)

a FASTA format of NRPS protein query sequence. b Secondary Structure Prediction of the NRPS protein of Streptomyces spp AJ8. (Color indicationsH Helix; S Strands and C Coil). c The predicted 3D model and the estimated global and local accuracy of the NRPS protein. d The structure alignment of NRPS between the first I-TASSER model and the top 5 most similar structure templates in PDB. e The predicted ligand-binding sites of the NRPS protein. (Fluorescent green yellow colour indicated the predicted ligand binding site)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Fig4: a FASTA format of NRPS protein query sequence. b Secondary Structure Prediction of the NRPS protein of Streptomyces spp AJ8. (Color indicationsH Helix; S Strands and C Coil). c The predicted 3D model and the estimated global and local accuracy of the NRPS protein. d The structure alignment of NRPS between the first I-TASSER model and the top 5 most similar structure templates in PDB. e The predicted ligand-binding sites of the NRPS protein. (Fluorescent green yellow colour indicated the predicted ligand binding site)
Mentions: The Fig. 4a, b shows the protein sequence and the predicted secondary structure of NRPS by I-TASSER analysis. The top five models predicted territory structure for NRPS protein by I-TASSER and the C-score were −4.03, −4.25, −3.36, −4095 and −3.36 respectively (Fig. 4c). The estimated TM-score and RMSD observed of 0.28 ± 0.09 and 15.8 ± 3.2 Å respectively. The TM-align structural alignment results revealed that, the top five PDB hits were 4nl6A, 1qonA, 2c3mB, 2fj0A and 1n35A and its TM scores were 0.843, 0.442, 0.432, 0.426 and 0.426 respectively. The five top most aligned proteins among the NRPS were spliceosome, acetylcholinesterase, ferredoxin oxidoreductases (PFOR), esterase and reovirus polymerase etc. (Fig. 4d). The multiple binding site ligands from different PDB hits (1zeiF, 2xmbA, 4ekdA, 3bz1H and 2hi8X) were confirmed as M-Cresol, Beta-l-Fucose, Cobalt (2+), Chlorophyll a and Bromide etc. (Fig. 4e; Table 4). The predicted EC numbers of the PDB hits weregiven in the Table 5. Based on the results revealed that, the five top most PDB enzyme hits were dipeptidyl carboxypeptidase Dcp, mouse acetylcholinesterase, Cys-418 thiylradical, human acetylcholinesterase and recombinant human butyrylcholinesterase.Fig. 4

Bottom Line: In vitro antagonistic activity results revealed that, Streptomyces spp.The genomic level identification revealed that, the strain was confirmed as Streptomyces spp.The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari District, 629502, Tamilnadu, India, adlinjenifer86@gmail.com.

ABSTRACT
Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The antimicrobial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, antifungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabolite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.

No MeSH data available.


Related in: MedlinePlus