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Haloalkaliphilic Streptomyces spp. AJ8 isolated from solar salt works and its' pharmacological potential.

Jenifer JS, Donio MB, Michaelbabu M, Vincent SG, Citarasu T - AMB Express (2015)

Bottom Line: In vitro antagonistic activity results revealed that, Streptomyces spp.The genomic level identification revealed that, the strain was confirmed as Streptomyces spp.The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari District, 629502, Tamilnadu, India, adlinjenifer86@gmail.com.

ABSTRACT
Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The antimicrobial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, antifungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabolite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of the nucleotide sequence of NRPS gene of haloalkaliphilic Streptomyces spp. AJ8 with that reported in other Streptomyces spp. by Neighbor Joining method using Geneious Pro analysis
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Fig3: Phylogenetic analysis of the nucleotide sequence of NRPS gene of haloalkaliphilic Streptomyces spp. AJ8 with that reported in other Streptomyces spp. by Neighbor Joining method using Geneious Pro analysis

Mentions: The NRPS gene specific PCR amplification generated a single gene fragment of 781 bp length and submitted to NCBI database (GenBank: KR491940.1). Multiple sequence alignment analysis revealed that, the NRPS gene of Streptomyces spp. AJ8 was more identical to the other NRPS family in Streptomyces spp. SAUK6068, S. coeruleoprunus NBRC15400, Streptomyces spp. AH1-5 and Streptomyces spp. 43-30-14 (Fig. 2). Phylogenetic and evolutionary analysis of the NRPS gene sequence revealed that, Streptomyces spp. AJ8 shared more than 80 % similarity to other Streptomyces spp. including Streptomyces spp. 35-45-7, Streptomyces spp. SAUK6068, S. coeruleoprunus NBRC15400, Streptomyces spp. NBRC15387 and Kribella spp. ID05-A0415 (Fig. 3).Fig. 2


Haloalkaliphilic Streptomyces spp. AJ8 isolated from solar salt works and its' pharmacological potential.

Jenifer JS, Donio MB, Michaelbabu M, Vincent SG, Citarasu T - AMB Express (2015)

Phylogenetic analysis of the nucleotide sequence of NRPS gene of haloalkaliphilic Streptomyces spp. AJ8 with that reported in other Streptomyces spp. by Neighbor Joining method using Geneious Pro analysis
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549370&req=5

Fig3: Phylogenetic analysis of the nucleotide sequence of NRPS gene of haloalkaliphilic Streptomyces spp. AJ8 with that reported in other Streptomyces spp. by Neighbor Joining method using Geneious Pro analysis
Mentions: The NRPS gene specific PCR amplification generated a single gene fragment of 781 bp length and submitted to NCBI database (GenBank: KR491940.1). Multiple sequence alignment analysis revealed that, the NRPS gene of Streptomyces spp. AJ8 was more identical to the other NRPS family in Streptomyces spp. SAUK6068, S. coeruleoprunus NBRC15400, Streptomyces spp. AH1-5 and Streptomyces spp. 43-30-14 (Fig. 2). Phylogenetic and evolutionary analysis of the NRPS gene sequence revealed that, Streptomyces spp. AJ8 shared more than 80 % similarity to other Streptomyces spp. including Streptomyces spp. 35-45-7, Streptomyces spp. SAUK6068, S. coeruleoprunus NBRC15400, Streptomyces spp. NBRC15387 and Kribella spp. ID05-A0415 (Fig. 3).Fig. 2

Bottom Line: In vitro antagonistic activity results revealed that, Streptomyces spp.The genomic level identification revealed that, the strain was confirmed as Streptomyces spp.The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition.

View Article: PubMed Central - PubMed

Affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari District, 629502, Tamilnadu, India, adlinjenifer86@gmail.com.

ABSTRACT
Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The antimicrobial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, antifungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabolite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.

No MeSH data available.


Related in: MedlinePlus