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Antitumor Activity of Doxorubicin-Loaded Carbon Nanotubes Incorporated Poly(Lactic-Co-Glycolic Acid) Electrospun Composite Nanofibers.

Yu Y, Kong L, Li L, Li N, Yan P - Nanoscale Res Lett (2015)

Bottom Line: The properties of the prepared composite nanofibrous mats were characterized by various techniques.The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers.More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, 264003, People's Republic of China.

ABSTRACT
The drug-loaded composite electrospun nanofiber has attracted more attention in biomedical field, especially in cancer therapy. In this study, a composite nanofiber was fabricated by electrospinning for cancer treatment. Firstly, the carbon nanotubes (CNTs) were selected as carriers to load the anticancer drug-doxorubicin (DOX) hydrochloride. Secondly, the DOX-loaded CNTs (DOX@CNTs) were incorporated into the poly(lactic-co-glycolic acid) (PLGA) nanofibers via electrospinning. Finally, a new drug-loaded nanofibrous scaffold (PLGA/DOX@CNTs) was formed. The properties of the prepared composite nanofibrous mats were characterized by various techniques. The release profiles of the different DOX-loaded nanofibers were measured, and the in vitro antitumor efficacy against HeLa cells was also evaluated. The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers. More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained. Thus, the prepared composite nanofibrous mats are a promising alternative for cancer treatment.

No MeSH data available.


Related in: MedlinePlus

Confocal laser scanning microscopy images of HeLa cells treated with PLLA/2 % CNTs, free DOX, PLLA/1.5 % DOX, and PLLA/1.5 % DOX@2 % CNTs. DOX concentration was 25 μg/mL. The red fluorescence indicates the released DOX. The green fluorescence represents Alexa Fluor 488® phalloidin-stained F-actin. Scale bars = 100 μm
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Fig8: Confocal laser scanning microscopy images of HeLa cells treated with PLLA/2 % CNTs, free DOX, PLLA/1.5 % DOX, and PLLA/1.5 % DOX@2 % CNTs. DOX concentration was 25 μg/mL. The red fluorescence indicates the released DOX. The green fluorescence represents Alexa Fluor 488® phalloidin-stained F-actin. Scale bars = 100 μm

Mentions: CLSM imaging technology was further employed to gain more insights into the therapeutic effect caused by PLGA/DOX@CNTs. The Alexa Fluor® 488 phalloidin was used to label cytoskeleton and the red fluorescent nature of DOX facilitated the in situ observation. As shown in Fig. 8, it is worth noting that the free DOX, PLGA/1.5 % DOX and PLGA/1.5 % DOX@2 % CNTs could effectively inhibited the growth of HeLa cells, because the drug treated cells displayed apparent morphological features of apoptosis after treatment for 24-h compared with control. The cells incubated with PLGA/2 % CNTs nanofibers showed no significant abnormality on the morphology, indicating that the cytotoxicity was associated with the DOX molecule, not the nanocarrier.Fig. 8


Antitumor Activity of Doxorubicin-Loaded Carbon Nanotubes Incorporated Poly(Lactic-Co-Glycolic Acid) Electrospun Composite Nanofibers.

Yu Y, Kong L, Li L, Li N, Yan P - Nanoscale Res Lett (2015)

Confocal laser scanning microscopy images of HeLa cells treated with PLLA/2 % CNTs, free DOX, PLLA/1.5 % DOX, and PLLA/1.5 % DOX@2 % CNTs. DOX concentration was 25 μg/mL. The red fluorescence indicates the released DOX. The green fluorescence represents Alexa Fluor 488® phalloidin-stained F-actin. Scale bars = 100 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4549354&req=5

Fig8: Confocal laser scanning microscopy images of HeLa cells treated with PLLA/2 % CNTs, free DOX, PLLA/1.5 % DOX, and PLLA/1.5 % DOX@2 % CNTs. DOX concentration was 25 μg/mL. The red fluorescence indicates the released DOX. The green fluorescence represents Alexa Fluor 488® phalloidin-stained F-actin. Scale bars = 100 μm
Mentions: CLSM imaging technology was further employed to gain more insights into the therapeutic effect caused by PLGA/DOX@CNTs. The Alexa Fluor® 488 phalloidin was used to label cytoskeleton and the red fluorescent nature of DOX facilitated the in situ observation. As shown in Fig. 8, it is worth noting that the free DOX, PLGA/1.5 % DOX and PLGA/1.5 % DOX@2 % CNTs could effectively inhibited the growth of HeLa cells, because the drug treated cells displayed apparent morphological features of apoptosis after treatment for 24-h compared with control. The cells incubated with PLGA/2 % CNTs nanofibers showed no significant abnormality on the morphology, indicating that the cytotoxicity was associated with the DOX molecule, not the nanocarrier.Fig. 8

Bottom Line: The properties of the prepared composite nanofibrous mats were characterized by various techniques.The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers.More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, 264003, People's Republic of China.

ABSTRACT
The drug-loaded composite electrospun nanofiber has attracted more attention in biomedical field, especially in cancer therapy. In this study, a composite nanofiber was fabricated by electrospinning for cancer treatment. Firstly, the carbon nanotubes (CNTs) were selected as carriers to load the anticancer drug-doxorubicin (DOX) hydrochloride. Secondly, the DOX-loaded CNTs (DOX@CNTs) were incorporated into the poly(lactic-co-glycolic acid) (PLGA) nanofibers via electrospinning. Finally, a new drug-loaded nanofibrous scaffold (PLGA/DOX@CNTs) was formed. The properties of the prepared composite nanofibrous mats were characterized by various techniques. The release profiles of the different DOX-loaded nanofibers were measured, and the in vitro antitumor efficacy against HeLa cells was also evaluated. The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers. More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained. Thus, the prepared composite nanofibrous mats are a promising alternative for cancer treatment.

No MeSH data available.


Related in: MedlinePlus