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Antitumor Activity of Doxorubicin-Loaded Carbon Nanotubes Incorporated Poly(Lactic-Co-Glycolic Acid) Electrospun Composite Nanofibers.

Yu Y, Kong L, Li L, Li N, Yan P - Nanoscale Res Lett (2015)

Bottom Line: The properties of the prepared composite nanofibrous mats were characterized by various techniques.The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers.More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, 264003, People's Republic of China.

ABSTRACT
The drug-loaded composite electrospun nanofiber has attracted more attention in biomedical field, especially in cancer therapy. In this study, a composite nanofiber was fabricated by electrospinning for cancer treatment. Firstly, the carbon nanotubes (CNTs) were selected as carriers to load the anticancer drug-doxorubicin (DOX) hydrochloride. Secondly, the DOX-loaded CNTs (DOX@CNTs) were incorporated into the poly(lactic-co-glycolic acid) (PLGA) nanofibers via electrospinning. Finally, a new drug-loaded nanofibrous scaffold (PLGA/DOX@CNTs) was formed. The properties of the prepared composite nanofibrous mats were characterized by various techniques. The release profiles of the different DOX-loaded nanofibers were measured, and the in vitro antitumor efficacy against HeLa cells was also evaluated. The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers. More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained. Thus, the prepared composite nanofibrous mats are a promising alternative for cancer treatment.

No MeSH data available.


The morphology and diameter distributions of PLGA and PLGA/DOX@CNTs composite nanofibers. SEM images of a PLLA/1.5 % DOX@0.5 % CNTs, b PLGA/1.5 % DOX@1 % CNTs, and c PLGA/1.5 % DOX@2 % CNTs nanofibers. d–f The corresponding TEM images of a–c. g–i The corresponding diameter distributions of a–c
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Fig2: The morphology and diameter distributions of PLGA and PLGA/DOX@CNTs composite nanofibers. SEM images of a PLLA/1.5 % DOX@0.5 % CNTs, b PLGA/1.5 % DOX@1 % CNTs, and c PLGA/1.5 % DOX@2 % CNTs nanofibers. d–f The corresponding TEM images of a–c. g–i The corresponding diameter distributions of a–c

Mentions: It is known that the morphology of electrospun nanofibers is influenced by various parameters, such as the concentration of polymers, applied voltage, and flow rate of solution. For the organic/inorganic composite nanofibers, the morphology and the diameter can be dramatically affected by the content of inorganic component in polymers [20, 26]. Therefore, it is encouraged to investigate the effects of the content of CNTs on the nanofibrous morphology and diameter. Figure 2 shows the morphology and diameter distribution of the electrospun nanofibers. As shown in Fig. 2a, it can be clearly seen that a smooth surface morphology and relatively uniform fibrous diameter were observed in PLGA/0.5 % CNTs, with the average diameter of 528.5 nm (Fig. 2g). When the content of CNTs was 1 % (Fig. 2b), a similar fiber morphology was seen. While the fiber diameter slightly increased, the average diameter of PLGA/1 % CNTs was 814.1 nm (Fig. 2h). When the content of CNTs was up to 2 %, the fibers of PLGA/2 % CNTs were changed to be swollen to a certain extent (Fig. 2c), but the fiber morphology still maintained. Accordingly, the average diameter of PLGA/2 % CNTs was increased to 1058.8 nm (Fig. 2i). As a result, the average diameter of the fibers gradually increased with increasing content of CNTs, which can be attributed to the increase in viscosity of the solution after the addition of a large amount of CNTs [26, 27]. Additionally, the TEM images of Fig. 2d–f clearly shows that CNTs can be homogenously distributed within the nanofibers, and more CNTs appeared with the increase of the CNTs content. Hence, 2 % of CNTs was an alternative in the following experiments.Fig. 2


Antitumor Activity of Doxorubicin-Loaded Carbon Nanotubes Incorporated Poly(Lactic-Co-Glycolic Acid) Electrospun Composite Nanofibers.

Yu Y, Kong L, Li L, Li N, Yan P - Nanoscale Res Lett (2015)

The morphology and diameter distributions of PLGA and PLGA/DOX@CNTs composite nanofibers. SEM images of a PLLA/1.5 % DOX@0.5 % CNTs, b PLGA/1.5 % DOX@1 % CNTs, and c PLGA/1.5 % DOX@2 % CNTs nanofibers. d–f The corresponding TEM images of a–c. g–i The corresponding diameter distributions of a–c
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Related In: Results  -  Collection

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Fig2: The morphology and diameter distributions of PLGA and PLGA/DOX@CNTs composite nanofibers. SEM images of a PLLA/1.5 % DOX@0.5 % CNTs, b PLGA/1.5 % DOX@1 % CNTs, and c PLGA/1.5 % DOX@2 % CNTs nanofibers. d–f The corresponding TEM images of a–c. g–i The corresponding diameter distributions of a–c
Mentions: It is known that the morphology of electrospun nanofibers is influenced by various parameters, such as the concentration of polymers, applied voltage, and flow rate of solution. For the organic/inorganic composite nanofibers, the morphology and the diameter can be dramatically affected by the content of inorganic component in polymers [20, 26]. Therefore, it is encouraged to investigate the effects of the content of CNTs on the nanofibrous morphology and diameter. Figure 2 shows the morphology and diameter distribution of the electrospun nanofibers. As shown in Fig. 2a, it can be clearly seen that a smooth surface morphology and relatively uniform fibrous diameter were observed in PLGA/0.5 % CNTs, with the average diameter of 528.5 nm (Fig. 2g). When the content of CNTs was 1 % (Fig. 2b), a similar fiber morphology was seen. While the fiber diameter slightly increased, the average diameter of PLGA/1 % CNTs was 814.1 nm (Fig. 2h). When the content of CNTs was up to 2 %, the fibers of PLGA/2 % CNTs were changed to be swollen to a certain extent (Fig. 2c), but the fiber morphology still maintained. Accordingly, the average diameter of PLGA/2 % CNTs was increased to 1058.8 nm (Fig. 2i). As a result, the average diameter of the fibers gradually increased with increasing content of CNTs, which can be attributed to the increase in viscosity of the solution after the addition of a large amount of CNTs [26, 27]. Additionally, the TEM images of Fig. 2d–f clearly shows that CNTs can be homogenously distributed within the nanofibers, and more CNTs appeared with the increase of the CNTs content. Hence, 2 % of CNTs was an alternative in the following experiments.Fig. 2

Bottom Line: The properties of the prepared composite nanofibrous mats were characterized by various techniques.The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers.More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, 264003, People's Republic of China.

ABSTRACT
The drug-loaded composite electrospun nanofiber has attracted more attention in biomedical field, especially in cancer therapy. In this study, a composite nanofiber was fabricated by electrospinning for cancer treatment. Firstly, the carbon nanotubes (CNTs) were selected as carriers to load the anticancer drug-doxorubicin (DOX) hydrochloride. Secondly, the DOX-loaded CNTs (DOX@CNTs) were incorporated into the poly(lactic-co-glycolic acid) (PLGA) nanofibers via electrospinning. Finally, a new drug-loaded nanofibrous scaffold (PLGA/DOX@CNTs) was formed. The properties of the prepared composite nanofibrous mats were characterized by various techniques. The release profiles of the different DOX-loaded nanofibers were measured, and the in vitro antitumor efficacy against HeLa cells was also evaluated. The results showed that DOX-loaded CNTs can be readily incorporated into the nanofibers with relatively uniform distribution within the nanofibers. More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained. Thus, the prepared composite nanofibrous mats are a promising alternative for cancer treatment.

No MeSH data available.