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Anesthesia and Monitoring in Small Laboratory Mammals Used in Anesthesiology, Respiratory and Critical Care Research: A Systematic Review on the Current Reporting in Top-10 Impact Factor Ranked Journals.

Uhlig C, Krause H, Koch T, Gama de Abreu M, Spieth PM - PLoS ONE (2015)

Bottom Line: Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively.Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low.These measures are of special interest when translating experimental findings to future clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Pulmonary Engineering Group, Department of Anesthesiology and Intensive Care Therapy, University Hospital Dresden, Dresden, Technische Universität Dresden, Germany.

ABSTRACT

Rationale: This study aimed to investigate the quality of reporting of anesthesia and euthanasia in experimental studies in small laboratory mammals published in the top ten impact factor journals.

Methods: A descriptive systematic review was conducted and data was abstracted from the ten highest ranked journals with respect to impact factor in the categories 'Anesthesiology', 'Critical Care Medicine' and 'Respiratory System' as defined by the 2012 Journal Citation Reports. Inclusion criteria according to PICOS criteria were as follows: 1) population: small laboratory mammals; 2) intervention: any form of anesthesia and/or euthanasia; 3) comparison: not specified; 4) primary outcome: type of anesthesia, anesthetic agents and type of euthanasia; secondary outcome: animal characteristics, monitoring, mechanical ventilation, fluid management, postoperative pain therapy, animal care approval, sample size calculation and performed interventions; 5) study: experimental studies. Anesthesia, euthanasia, and monitoring were analyzed per performed intervention in each article.

Results: The search yielded 845 articles with 1,041 interventions of interest. Throughout the manuscripts we found poor quality and frequency of reporting with respect to completeness of data on animal characteristics as well as euthanasia, while anesthesia (732/1041, 70.3%) and interventions without survival (970/1041, 93.2%) per se were frequently reported. Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively. Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low. Euthanasia practice was reported as anesthesia (348/501, 69%), transcardial perfusion (37/501, 8%), carbon dioxide (26/501, 6%), decapitation (22/501, 5%), exsanguination (23/501, 5%), other (25/501, 5%) and not specified (20/501, 4%, respectively.

Conclusions: The present systematic review revealed insufficient reporting of anesthesia and euthanasia methods throughout experimental studies in small laboratory mammals. Specific guidelines for anesthesia and euthanasia regimens should be considered to achieve comparability, quality of animal experiments and animal welfare. These measures are of special interest when translating experimental findings to future clinical applications.

No MeSH data available.


Related in: MedlinePlus

Frequencies of reported species.Values are given as number; percentage relative to total (below pie diagram). Analysis was performed per publication. The total number of 897 is explained by use of multiple species among manuscripts.
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pone.0134205.g002: Frequencies of reported species.Values are given as number; percentage relative to total (below pie diagram). Analysis was performed per publication. The total number of 897 is explained by use of multiple species among manuscripts.

Mentions: Animal characteristics are described in Table B in S2 File and Fig 2. Animal species and breeding strains are summarized in Table 3. Frequency of reporting, with respect to journal category and impact factor, is shown in Table 4. The total number of small laboratory mammals used was not reported in 533/897 (59.4%) and not specified in 15/897 (1.7%) data sets out of 845 analyzed manuscripts. The difference between data sets and analyzed manuscripts results from the possible use of multiple species in a single manuscript. A total number of 23,009 small laboratory mammals, including 6,570/23,009 (28.5%) mice, 15,729/23,009 (68.4%) rats, 40/23,009 (0.2%) guinea pigs and 670/23,009 (2.9%) rabbits was described in the remaining 349/897 (38.9%) interventions involving different small laboratory species.


Anesthesia and Monitoring in Small Laboratory Mammals Used in Anesthesiology, Respiratory and Critical Care Research: A Systematic Review on the Current Reporting in Top-10 Impact Factor Ranked Journals.

Uhlig C, Krause H, Koch T, Gama de Abreu M, Spieth PM - PLoS ONE (2015)

Frequencies of reported species.Values are given as number; percentage relative to total (below pie diagram). Analysis was performed per publication. The total number of 897 is explained by use of multiple species among manuscripts.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549323&req=5

pone.0134205.g002: Frequencies of reported species.Values are given as number; percentage relative to total (below pie diagram). Analysis was performed per publication. The total number of 897 is explained by use of multiple species among manuscripts.
Mentions: Animal characteristics are described in Table B in S2 File and Fig 2. Animal species and breeding strains are summarized in Table 3. Frequency of reporting, with respect to journal category and impact factor, is shown in Table 4. The total number of small laboratory mammals used was not reported in 533/897 (59.4%) and not specified in 15/897 (1.7%) data sets out of 845 analyzed manuscripts. The difference between data sets and analyzed manuscripts results from the possible use of multiple species in a single manuscript. A total number of 23,009 small laboratory mammals, including 6,570/23,009 (28.5%) mice, 15,729/23,009 (68.4%) rats, 40/23,009 (0.2%) guinea pigs and 670/23,009 (2.9%) rabbits was described in the remaining 349/897 (38.9%) interventions involving different small laboratory species.

Bottom Line: Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively.Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low.These measures are of special interest when translating experimental findings to future clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Pulmonary Engineering Group, Department of Anesthesiology and Intensive Care Therapy, University Hospital Dresden, Dresden, Technische Universität Dresden, Germany.

ABSTRACT

Rationale: This study aimed to investigate the quality of reporting of anesthesia and euthanasia in experimental studies in small laboratory mammals published in the top ten impact factor journals.

Methods: A descriptive systematic review was conducted and data was abstracted from the ten highest ranked journals with respect to impact factor in the categories 'Anesthesiology', 'Critical Care Medicine' and 'Respiratory System' as defined by the 2012 Journal Citation Reports. Inclusion criteria according to PICOS criteria were as follows: 1) population: small laboratory mammals; 2) intervention: any form of anesthesia and/or euthanasia; 3) comparison: not specified; 4) primary outcome: type of anesthesia, anesthetic agents and type of euthanasia; secondary outcome: animal characteristics, monitoring, mechanical ventilation, fluid management, postoperative pain therapy, animal care approval, sample size calculation and performed interventions; 5) study: experimental studies. Anesthesia, euthanasia, and monitoring were analyzed per performed intervention in each article.

Results: The search yielded 845 articles with 1,041 interventions of interest. Throughout the manuscripts we found poor quality and frequency of reporting with respect to completeness of data on animal characteristics as well as euthanasia, while anesthesia (732/1041, 70.3%) and interventions without survival (970/1041, 93.2%) per se were frequently reported. Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively. Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low. Euthanasia practice was reported as anesthesia (348/501, 69%), transcardial perfusion (37/501, 8%), carbon dioxide (26/501, 6%), decapitation (22/501, 5%), exsanguination (23/501, 5%), other (25/501, 5%) and not specified (20/501, 4%, respectively.

Conclusions: The present systematic review revealed insufficient reporting of anesthesia and euthanasia methods throughout experimental studies in small laboratory mammals. Specific guidelines for anesthesia and euthanasia regimens should be considered to achieve comparability, quality of animal experiments and animal welfare. These measures are of special interest when translating experimental findings to future clinical applications.

No MeSH data available.


Related in: MedlinePlus