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Anesthesia and Monitoring in Small Laboratory Mammals Used in Anesthesiology, Respiratory and Critical Care Research: A Systematic Review on the Current Reporting in Top-10 Impact Factor Ranked Journals.

Uhlig C, Krause H, Koch T, Gama de Abreu M, Spieth PM - PLoS ONE (2015)

Bottom Line: Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively.Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low.These measures are of special interest when translating experimental findings to future clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Pulmonary Engineering Group, Department of Anesthesiology and Intensive Care Therapy, University Hospital Dresden, Dresden, Technische Universit├Ąt Dresden, Germany.

ABSTRACT

Rationale: This study aimed to investigate the quality of reporting of anesthesia and euthanasia in experimental studies in small laboratory mammals published in the top ten impact factor journals.

Methods: A descriptive systematic review was conducted and data was abstracted from the ten highest ranked journals with respect to impact factor in the categories 'Anesthesiology', 'Critical Care Medicine' and 'Respiratory System' as defined by the 2012 Journal Citation Reports. Inclusion criteria according to PICOS criteria were as follows: 1) population: small laboratory mammals; 2) intervention: any form of anesthesia and/or euthanasia; 3) comparison: not specified; 4) primary outcome: type of anesthesia, anesthetic agents and type of euthanasia; secondary outcome: animal characteristics, monitoring, mechanical ventilation, fluid management, postoperative pain therapy, animal care approval, sample size calculation and performed interventions; 5) study: experimental studies. Anesthesia, euthanasia, and monitoring were analyzed per performed intervention in each article.

Results: The search yielded 845 articles with 1,041 interventions of interest. Throughout the manuscripts we found poor quality and frequency of reporting with respect to completeness of data on animal characteristics as well as euthanasia, while anesthesia (732/1041, 70.3%) and interventions without survival (970/1041, 93.2%) per se were frequently reported. Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively. Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low. Euthanasia practice was reported as anesthesia (348/501, 69%), transcardial perfusion (37/501, 8%), carbon dioxide (26/501, 6%), decapitation (22/501, 5%), exsanguination (23/501, 5%), other (25/501, 5%) and not specified (20/501, 4%, respectively.

Conclusions: The present systematic review revealed insufficient reporting of anesthesia and euthanasia methods throughout experimental studies in small laboratory mammals. Specific guidelines for anesthesia and euthanasia regimens should be considered to achieve comparability, quality of animal experiments and animal welfare. These measures are of special interest when translating experimental findings to future clinical applications.

No MeSH data available.


Related in: MedlinePlus

Flowchart.Studies enrolled for this systematic review. *: small laboratory animals (clade glires).
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pone.0134205.g001: Flowchart.Studies enrolled for this systematic review. *: small laboratory animals (clade glires).

Mentions: The search yielded 5,335 experimental studies in total including 865 manuscripts describing experimental studies in small laboratory mammals. Seven of these 865 manuscripts screened in full text were excluded because neither small laboratory mammals nor cells were. Four more articles were excluded because cells were not primarily isolated, but commercially purchased. Nine papers involving rodents did not report anesthesia or euthanasia. Those nine manuscripts were excluded, too. Finally, 845 articles were included in this review. Terminal experiments were conducted in 806 (95.4%) articles. 1,041 different interventions involving anesthesia and/or euthanasia of small animals were performed. Initial survival of the intervention was reported in 455/1,041 (43.7%) of the performed interventions (Table 2). Anesthesia was reported in 599/845 (70.8%) of manuscripts and performed in 732/1,041 (70.3%) of the interventions. Euthanasia was applied in 970/1,041 (93.1%) of the interventions including interventions with initial survival. The flowchart of the selection process is depicted in Fig 1.


Anesthesia and Monitoring in Small Laboratory Mammals Used in Anesthesiology, Respiratory and Critical Care Research: A Systematic Review on the Current Reporting in Top-10 Impact Factor Ranked Journals.

Uhlig C, Krause H, Koch T, Gama de Abreu M, Spieth PM - PLoS ONE (2015)

Flowchart.Studies enrolled for this systematic review. *: small laboratory animals (clade glires).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549323&req=5

pone.0134205.g001: Flowchart.Studies enrolled for this systematic review. *: small laboratory animals (clade glires).
Mentions: The search yielded 5,335 experimental studies in total including 865 manuscripts describing experimental studies in small laboratory mammals. Seven of these 865 manuscripts screened in full text were excluded because neither small laboratory mammals nor cells were. Four more articles were excluded because cells were not primarily isolated, but commercially purchased. Nine papers involving rodents did not report anesthesia or euthanasia. Those nine manuscripts were excluded, too. Finally, 845 articles were included in this review. Terminal experiments were conducted in 806 (95.4%) articles. 1,041 different interventions involving anesthesia and/or euthanasia of small animals were performed. Initial survival of the intervention was reported in 455/1,041 (43.7%) of the performed interventions (Table 2). Anesthesia was reported in 599/845 (70.8%) of manuscripts and performed in 732/1,041 (70.3%) of the interventions. Euthanasia was applied in 970/1,041 (93.1%) of the interventions including interventions with initial survival. The flowchart of the selection process is depicted in Fig 1.

Bottom Line: Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively.Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low.These measures are of special interest when translating experimental findings to future clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Pulmonary Engineering Group, Department of Anesthesiology and Intensive Care Therapy, University Hospital Dresden, Dresden, Technische Universit├Ąt Dresden, Germany.

ABSTRACT

Rationale: This study aimed to investigate the quality of reporting of anesthesia and euthanasia in experimental studies in small laboratory mammals published in the top ten impact factor journals.

Methods: A descriptive systematic review was conducted and data was abstracted from the ten highest ranked journals with respect to impact factor in the categories 'Anesthesiology', 'Critical Care Medicine' and 'Respiratory System' as defined by the 2012 Journal Citation Reports. Inclusion criteria according to PICOS criteria were as follows: 1) population: small laboratory mammals; 2) intervention: any form of anesthesia and/or euthanasia; 3) comparison: not specified; 4) primary outcome: type of anesthesia, anesthetic agents and type of euthanasia; secondary outcome: animal characteristics, monitoring, mechanical ventilation, fluid management, postoperative pain therapy, animal care approval, sample size calculation and performed interventions; 5) study: experimental studies. Anesthesia, euthanasia, and monitoring were analyzed per performed intervention in each article.

Results: The search yielded 845 articles with 1,041 interventions of interest. Throughout the manuscripts we found poor quality and frequency of reporting with respect to completeness of data on animal characteristics as well as euthanasia, while anesthesia (732/1041, 70.3%) and interventions without survival (970/1041, 93.2%) per se were frequently reported. Premedication and neuromuscular blocking agents were reported in 169/732 (23.1%) and 38/732 (5.2%) interventions, respectively. Frequency of reporting of analgesia during (117/610, 19.1%) and after painful procedures (38/364, 10.4%) was low. Euthanasia practice was reported as anesthesia (348/501, 69%), transcardial perfusion (37/501, 8%), carbon dioxide (26/501, 6%), decapitation (22/501, 5%), exsanguination (23/501, 5%), other (25/501, 5%) and not specified (20/501, 4%, respectively.

Conclusions: The present systematic review revealed insufficient reporting of anesthesia and euthanasia methods throughout experimental studies in small laboratory mammals. Specific guidelines for anesthesia and euthanasia regimens should be considered to achieve comparability, quality of animal experiments and animal welfare. These measures are of special interest when translating experimental findings to future clinical applications.

No MeSH data available.


Related in: MedlinePlus