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A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis).

He L, Li Q, Liu L, Wang Y, Xie J, Yang H, Wang Q - PLoS ONE (2015)

Bottom Line: The accessory gland (AG) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some AG proteins bind to the spermatozoa and affect its function and properties.An extensive description of the AG proteome will help provide the basis for a better understanding of a number of reproductive mechanisms, including potentially spermatophore breakdown, dynamic functional and morphological changes in sperm cells and sperm acrosin enzyme vitality.Thus, the comprehensive catalog of proteins presented here can serve as a valuable reference for future studies of sperm maturation and regulatory mechanisms involved in crustacean reproduction.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, East China Normal University, Shanghai 200241, China.

ABSTRACT
The accessory gland (AG) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some AG proteins bind to the spermatozoa and affect its function and properties. Here we report the first comprehensive catalog of the AG secreted fluid during the mature phase of the Chinese mitten crab (Eriocheir sinensis). AG proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Altogether, the mass spectra of 1173 peptides were detected (1067 without decoy and contaminants) which allowed for the identification of 486 different proteins annotated upon the NCBI database (http://www.ncbi.nlm.nih.gov/) and our transcritptome dataset. The mass spectrometry proteomics data have been deposited at the ProteomeXchange with identifier PXD000700. An extensive description of the AG proteome will help provide the basis for a better understanding of a number of reproductive mechanisms, including potentially spermatophore breakdown, dynamic functional and morphological changes in sperm cells and sperm acrosin enzyme vitality. Thus, the comprehensive catalog of proteins presented here can serve as a valuable reference for future studies of sperm maturation and regulatory mechanisms involved in crustacean reproduction.

No MeSH data available.


Related in: MedlinePlus

Photograph of the male reproductive system in the E. sinensis.The male reproductive system of this crab consists of a pair of testes (T), vas deferens (VD), seminal vesicles (SV), accessory glands (AG) and ejaculatory duct (ED).
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pone.0136266.g001: Photograph of the male reproductive system in the E. sinensis.The male reproductive system of this crab consists of a pair of testes (T), vas deferens (VD), seminal vesicles (SV), accessory glands (AG) and ejaculatory duct (ED).

Mentions: Eriocheir sinensis, the Chinese mitten crab (Milne Edwards, 1853) is an economically important aquaculture species in China. Research on male reproductive biology of this crab mainly has focused on its spermatogenesis, spermatophore formation, spermatozoa ultrastructure, or spermatozoa metabolism. Transcriptome analysis of Chinese mitten crab testis [5] and AG [6] are newly available, opening up opportunities to learn more about these tissues’ functions. In the E. sinensis male, the reproductive system consists of a pair of testes with vas deferens, seminal vesicles, a pair of AG and a single ejaculatory duct (Fig 1). Mature male Chinese mitten crabs have a pair of well-developed AGs that open at the junction of seminal vesicles and the ejaculatory duct. Secretions of the AGs, along with spermatophores from the seminal vesicle and spermatic fluid, enter into the female spermatheca via the ejaculatory duct during mating. Within the female spermatheca, the spermatophores are gradually broken down, and free spermatozoa are released to encounter oocytes, leading to fertilization [7]. In a previous study, we found that the AG secreted protein can effectively digest the spermatophore wall to release free spermatozoa and interact with proteins from the spermatheca to increase the acrosomal enzyme activity of free spermatozoa [8]. We also found that there are many small and large vesicles in the crab AG [9], that are visible under transmission and scanning electron microscopy. These vesicles are thought to contain the enzymatic proteins or other activation factors, which may be required for spermatophore rupture or other function in fertilization.


A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis).

He L, Li Q, Liu L, Wang Y, Xie J, Yang H, Wang Q - PLoS ONE (2015)

Photograph of the male reproductive system in the E. sinensis.The male reproductive system of this crab consists of a pair of testes (T), vas deferens (VD), seminal vesicles (SV), accessory glands (AG) and ejaculatory duct (ED).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549300&req=5

pone.0136266.g001: Photograph of the male reproductive system in the E. sinensis.The male reproductive system of this crab consists of a pair of testes (T), vas deferens (VD), seminal vesicles (SV), accessory glands (AG) and ejaculatory duct (ED).
Mentions: Eriocheir sinensis, the Chinese mitten crab (Milne Edwards, 1853) is an economically important aquaculture species in China. Research on male reproductive biology of this crab mainly has focused on its spermatogenesis, spermatophore formation, spermatozoa ultrastructure, or spermatozoa metabolism. Transcriptome analysis of Chinese mitten crab testis [5] and AG [6] are newly available, opening up opportunities to learn more about these tissues’ functions. In the E. sinensis male, the reproductive system consists of a pair of testes with vas deferens, seminal vesicles, a pair of AG and a single ejaculatory duct (Fig 1). Mature male Chinese mitten crabs have a pair of well-developed AGs that open at the junction of seminal vesicles and the ejaculatory duct. Secretions of the AGs, along with spermatophores from the seminal vesicle and spermatic fluid, enter into the female spermatheca via the ejaculatory duct during mating. Within the female spermatheca, the spermatophores are gradually broken down, and free spermatozoa are released to encounter oocytes, leading to fertilization [7]. In a previous study, we found that the AG secreted protein can effectively digest the spermatophore wall to release free spermatozoa and interact with proteins from the spermatheca to increase the acrosomal enzyme activity of free spermatozoa [8]. We also found that there are many small and large vesicles in the crab AG [9], that are visible under transmission and scanning electron microscopy. These vesicles are thought to contain the enzymatic proteins or other activation factors, which may be required for spermatophore rupture or other function in fertilization.

Bottom Line: The accessory gland (AG) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some AG proteins bind to the spermatozoa and affect its function and properties.An extensive description of the AG proteome will help provide the basis for a better understanding of a number of reproductive mechanisms, including potentially spermatophore breakdown, dynamic functional and morphological changes in sperm cells and sperm acrosin enzyme vitality.Thus, the comprehensive catalog of proteins presented here can serve as a valuable reference for future studies of sperm maturation and regulatory mechanisms involved in crustacean reproduction.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, East China Normal University, Shanghai 200241, China.

ABSTRACT
The accessory gland (AG) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some AG proteins bind to the spermatozoa and affect its function and properties. Here we report the first comprehensive catalog of the AG secreted fluid during the mature phase of the Chinese mitten crab (Eriocheir sinensis). AG proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Altogether, the mass spectra of 1173 peptides were detected (1067 without decoy and contaminants) which allowed for the identification of 486 different proteins annotated upon the NCBI database (http://www.ncbi.nlm.nih.gov/) and our transcritptome dataset. The mass spectrometry proteomics data have been deposited at the ProteomeXchange with identifier PXD000700. An extensive description of the AG proteome will help provide the basis for a better understanding of a number of reproductive mechanisms, including potentially spermatophore breakdown, dynamic functional and morphological changes in sperm cells and sperm acrosin enzyme vitality. Thus, the comprehensive catalog of proteins presented here can serve as a valuable reference for future studies of sperm maturation and regulatory mechanisms involved in crustacean reproduction.

No MeSH data available.


Related in: MedlinePlus