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The Wheat NAC Transcription Factor TaNAC2L Is Regulated at the Transcriptional and Post-Translational Levels and Promotes Heat Stress Tolerance in Transgenic Arabidopsis.

Guo W, Zhang J, Zhang N, Xin M, Peng H, Hu Z, Ni Z, Du J - PLoS ONE (2015)

Bottom Line: In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L.High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions.Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China; National Plant Gene Research Centre (Beijing), Beijing, 100193, China.

ABSTRACT
Heat stress poses a serious threat to global crop production. In efforts that aim to mitigate the adverse effects of heat stress on crops, a variety of genetic tools are being used to develop plants with improved thermotolerance. The characterization of important regulators of heat stress tolerance provides essential information for this aim. In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L. High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions. TaNAC2L overexpression also activated the expression of heat-related genes in the transgenic Arabidopsis plants, suggesting that TaNAC2L may improve heat tolerance by regulating the expression of stress-responsive genes. Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway. Thus, this wheat transcription factor may have potential uses in enhancing thermotolerance in crops.

No MeSH data available.


Related in: MedlinePlus

Hypocotyl elongation of TaNAC2L-overexpressing transgenic Arabidopsis plants.A. Levels of the TaNAC2L transcript in the wild type (WT) and 20 transgenic Arabidopsis lines according to real-time quantitative PCR analyses. B. Hypocotyl elongation of WT and transgenic plants (#13) after growth for 2.5 days in the dark at 22°C. Seedling treatments included: 1) maintained at 22°C; 2) treated at 38°C for 90 min; 3) treated at 45°C for 2 h; 4) first treated at 38°C for 90 min followed by 2 h at 22°C and then 2 h at 45°C. For all treatments, seedlings were returned to 22°C for 2.5 days and then photographed. The quantitative analysis of the hypocotyl length of the wild-type and transgenic plants is presented below the photographs.
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pone.0135667.g002: Hypocotyl elongation of TaNAC2L-overexpressing transgenic Arabidopsis plants.A. Levels of the TaNAC2L transcript in the wild type (WT) and 20 transgenic Arabidopsis lines according to real-time quantitative PCR analyses. B. Hypocotyl elongation of WT and transgenic plants (#13) after growth for 2.5 days in the dark at 22°C. Seedling treatments included: 1) maintained at 22°C; 2) treated at 38°C for 90 min; 3) treated at 45°C for 2 h; 4) first treated at 38°C for 90 min followed by 2 h at 22°C and then 2 h at 45°C. For all treatments, seedlings were returned to 22°C for 2.5 days and then photographed. The quantitative analysis of the hypocotyl length of the wild-type and transgenic plants is presented below the photographs.

Mentions: To investigate the function of TaNAC2L in heat tolerance, we generated transgenic Arabidopsis plants that overexpress TaNAC2L. The TaNAC2L produced from this construct was tagged with hemagglutinin (HA) at the N-terminus and expressed under the control of the 35S promoter. A total of 20 transgenic lines were obtained and the expression levels of TaNAC2L were determined. Three homozygous transgenic lines (TaNAC2L-OX #13, #9, and #2) with higher expression levels of TaNAC2L were investigated further (Fig 2A).


The Wheat NAC Transcription Factor TaNAC2L Is Regulated at the Transcriptional and Post-Translational Levels and Promotes Heat Stress Tolerance in Transgenic Arabidopsis.

Guo W, Zhang J, Zhang N, Xin M, Peng H, Hu Z, Ni Z, Du J - PLoS ONE (2015)

Hypocotyl elongation of TaNAC2L-overexpressing transgenic Arabidopsis plants.A. Levels of the TaNAC2L transcript in the wild type (WT) and 20 transgenic Arabidopsis lines according to real-time quantitative PCR analyses. B. Hypocotyl elongation of WT and transgenic plants (#13) after growth for 2.5 days in the dark at 22°C. Seedling treatments included: 1) maintained at 22°C; 2) treated at 38°C for 90 min; 3) treated at 45°C for 2 h; 4) first treated at 38°C for 90 min followed by 2 h at 22°C and then 2 h at 45°C. For all treatments, seedlings were returned to 22°C for 2.5 days and then photographed. The quantitative analysis of the hypocotyl length of the wild-type and transgenic plants is presented below the photographs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549282&req=5

pone.0135667.g002: Hypocotyl elongation of TaNAC2L-overexpressing transgenic Arabidopsis plants.A. Levels of the TaNAC2L transcript in the wild type (WT) and 20 transgenic Arabidopsis lines according to real-time quantitative PCR analyses. B. Hypocotyl elongation of WT and transgenic plants (#13) after growth for 2.5 days in the dark at 22°C. Seedling treatments included: 1) maintained at 22°C; 2) treated at 38°C for 90 min; 3) treated at 45°C for 2 h; 4) first treated at 38°C for 90 min followed by 2 h at 22°C and then 2 h at 45°C. For all treatments, seedlings were returned to 22°C for 2.5 days and then photographed. The quantitative analysis of the hypocotyl length of the wild-type and transgenic plants is presented below the photographs.
Mentions: To investigate the function of TaNAC2L in heat tolerance, we generated transgenic Arabidopsis plants that overexpress TaNAC2L. The TaNAC2L produced from this construct was tagged with hemagglutinin (HA) at the N-terminus and expressed under the control of the 35S promoter. A total of 20 transgenic lines were obtained and the expression levels of TaNAC2L were determined. Three homozygous transgenic lines (TaNAC2L-OX #13, #9, and #2) with higher expression levels of TaNAC2L were investigated further (Fig 2A).

Bottom Line: In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L.High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions.Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China; National Plant Gene Research Centre (Beijing), Beijing, 100193, China.

ABSTRACT
Heat stress poses a serious threat to global crop production. In efforts that aim to mitigate the adverse effects of heat stress on crops, a variety of genetic tools are being used to develop plants with improved thermotolerance. The characterization of important regulators of heat stress tolerance provides essential information for this aim. In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L. High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions. TaNAC2L overexpression also activated the expression of heat-related genes in the transgenic Arabidopsis plants, suggesting that TaNAC2L may improve heat tolerance by regulating the expression of stress-responsive genes. Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway. Thus, this wheat transcription factor may have potential uses in enhancing thermotolerance in crops.

No MeSH data available.


Related in: MedlinePlus