Limits...
The Wheat NAC Transcription Factor TaNAC2L Is Regulated at the Transcriptional and Post-Translational Levels and Promotes Heat Stress Tolerance in Transgenic Arabidopsis.

Guo W, Zhang J, Zhang N, Xin M, Peng H, Hu Z, Ni Z, Du J - PLoS ONE (2015)

Bottom Line: In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L.High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions.Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China; National Plant Gene Research Centre (Beijing), Beijing, 100193, China.

ABSTRACT
Heat stress poses a serious threat to global crop production. In efforts that aim to mitigate the adverse effects of heat stress on crops, a variety of genetic tools are being used to develop plants with improved thermotolerance. The characterization of important regulators of heat stress tolerance provides essential information for this aim. In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L. High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions. TaNAC2L overexpression also activated the expression of heat-related genes in the transgenic Arabidopsis plants, suggesting that TaNAC2L may improve heat tolerance by regulating the expression of stress-responsive genes. Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway. Thus, this wheat transcription factor may have potential uses in enhancing thermotolerance in crops.

No MeSH data available.


Related in: MedlinePlus

Sequence alignment of TaNAC2L to wheat NAC proteins and TaNAC2L expression in response to heat stress.A. Amino acid alignment of TaNAC2L and eight other wheat NAC proteins. The black boxes indicate identical residues and the underlined region indicates the conserved NAC domain. B. Expression of TaNAC2L under heat stress. Three-leaf seedlings of the common wheat cultivar Chinese Spring (CS) and the heat-tolerant cultivar TAM107 were exposed to a 40°C high temperature treatment and then recovered in normal conditions after 2 h of heat stress.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4549282&req=5

pone.0135667.g001: Sequence alignment of TaNAC2L to wheat NAC proteins and TaNAC2L expression in response to heat stress.A. Amino acid alignment of TaNAC2L and eight other wheat NAC proteins. The black boxes indicate identical residues and the underlined region indicates the conserved NAC domain. B. Expression of TaNAC2L under heat stress. Three-leaf seedlings of the common wheat cultivar Chinese Spring (CS) and the heat-tolerant cultivar TAM107 were exposed to a 40°C high temperature treatment and then recovered in normal conditions after 2 h of heat stress.

Mentions: Our previous high-throughput sequencing analysis identified one transcript that exhibited significant upregulation after heat stress. The amino acid sequence of this transcript was aligned to known wheat NAC proteins and the results indicated that it differed from TaNAC2, TaNAC2A, TaNAC2B, and TaNAC2D by only 3–4 amino acids; we named this transcript TaNAC2L (Fig 1A). We next used BLAST to compare TaNAC2L to the assembled wheat genome sequences from IWGSC (http://www.wheatgenome.org/) and found that it showed the highest similarity to the contig on chromosome 5B. TaNAC2 had been mapped on chromosome 5A [16]; thus, these results indicated that TaNAC2L is a homoeolog of TaNAC2. To further investigate the expression of TaNAC2L during heat stress, real-time quantitative PCR was performed after exposing the heat-sensitive wheat cultivar Chinese Spring (CS), and the heat-tolerant cultivar, TAM107, to normal growth conditions or a 40°C high-temperature treatment. As shown in Fig 1B, transcripts of TaNAC2L were present at extremely low levels in both the CS and TAM107 cultivars under normal growth conditions and the response to high temperature differed between the two cultivars. In the heat-sensitive cultivar CS, TaNAC2L transcript levels peaked at 1 h after heat stress and then returned to normal levels after the plants recovered. In the heat-tolerant cultivar TAM107, TaNAC2L peaked earlier, at 0.5 h after heat stress, and then decreased gradually until 2 h. We speculate that the rapid increase in TaNAC2L transcript abundance might be associated with heat tolerance in TAM107.


The Wheat NAC Transcription Factor TaNAC2L Is Regulated at the Transcriptional and Post-Translational Levels and Promotes Heat Stress Tolerance in Transgenic Arabidopsis.

Guo W, Zhang J, Zhang N, Xin M, Peng H, Hu Z, Ni Z, Du J - PLoS ONE (2015)

Sequence alignment of TaNAC2L to wheat NAC proteins and TaNAC2L expression in response to heat stress.A. Amino acid alignment of TaNAC2L and eight other wheat NAC proteins. The black boxes indicate identical residues and the underlined region indicates the conserved NAC domain. B. Expression of TaNAC2L under heat stress. Three-leaf seedlings of the common wheat cultivar Chinese Spring (CS) and the heat-tolerant cultivar TAM107 were exposed to a 40°C high temperature treatment and then recovered in normal conditions after 2 h of heat stress.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549282&req=5

pone.0135667.g001: Sequence alignment of TaNAC2L to wheat NAC proteins and TaNAC2L expression in response to heat stress.A. Amino acid alignment of TaNAC2L and eight other wheat NAC proteins. The black boxes indicate identical residues and the underlined region indicates the conserved NAC domain. B. Expression of TaNAC2L under heat stress. Three-leaf seedlings of the common wheat cultivar Chinese Spring (CS) and the heat-tolerant cultivar TAM107 were exposed to a 40°C high temperature treatment and then recovered in normal conditions after 2 h of heat stress.
Mentions: Our previous high-throughput sequencing analysis identified one transcript that exhibited significant upregulation after heat stress. The amino acid sequence of this transcript was aligned to known wheat NAC proteins and the results indicated that it differed from TaNAC2, TaNAC2A, TaNAC2B, and TaNAC2D by only 3–4 amino acids; we named this transcript TaNAC2L (Fig 1A). We next used BLAST to compare TaNAC2L to the assembled wheat genome sequences from IWGSC (http://www.wheatgenome.org/) and found that it showed the highest similarity to the contig on chromosome 5B. TaNAC2 had been mapped on chromosome 5A [16]; thus, these results indicated that TaNAC2L is a homoeolog of TaNAC2. To further investigate the expression of TaNAC2L during heat stress, real-time quantitative PCR was performed after exposing the heat-sensitive wheat cultivar Chinese Spring (CS), and the heat-tolerant cultivar, TAM107, to normal growth conditions or a 40°C high-temperature treatment. As shown in Fig 1B, transcripts of TaNAC2L were present at extremely low levels in both the CS and TAM107 cultivars under normal growth conditions and the response to high temperature differed between the two cultivars. In the heat-sensitive cultivar CS, TaNAC2L transcript levels peaked at 1 h after heat stress and then returned to normal levels after the plants recovered. In the heat-tolerant cultivar TAM107, TaNAC2L peaked earlier, at 0.5 h after heat stress, and then decreased gradually until 2 h. We speculate that the rapid increase in TaNAC2L transcript abundance might be associated with heat tolerance in TAM107.

Bottom Line: In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L.High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions.Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China; National Plant Gene Research Centre (Beijing), Beijing, 100193, China.

ABSTRACT
Heat stress poses a serious threat to global crop production. In efforts that aim to mitigate the adverse effects of heat stress on crops, a variety of genetic tools are being used to develop plants with improved thermotolerance. The characterization of important regulators of heat stress tolerance provides essential information for this aim. In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L. High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions. TaNAC2L overexpression also activated the expression of heat-related genes in the transgenic Arabidopsis plants, suggesting that TaNAC2L may improve heat tolerance by regulating the expression of stress-responsive genes. Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway. Thus, this wheat transcription factor may have potential uses in enhancing thermotolerance in crops.

No MeSH data available.


Related in: MedlinePlus