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Prostate Tumor Overexpressed 1 (PTOV1) Is a Novel Prognostic Marker for Nasopharyngeal Carcinoma Progression and Poor Survival Outcomes.

Yang Q, Lin H, Wu S, Lei F, Zhu X, Song L, Hong M, Guo L - PLoS ONE (2015)

Bottom Line: Patients with a higher PTOV1 expression had shorter overall survival compared with those with a lower PTOV1 expression level, especially in lower N stage patients.Our data demonstrated that PTOV1 overexpression is associated with poor survival outcomes of NPC patients, especially in N0-1 patients.Hence, PTOV1 may help to detect early lymph node metastasis of NPC patients and serve as an independent prognostic biomarker for human NPC.

View Article: PubMed Central - PubMed

Affiliation: Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, P.R. China; Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, Guangzhou, 510060, P.R. China.

ABSTRACT

Background: Prostate tumor overexpressed 1 (PTOV1) has been reported to contribute to increased cancer proliferation. However, the clinical significance of PTOV1 in the development and progression of nasopharyngeal carcinoma (NPC) is unclear. Our study aimed to investigate the expression pattern of PTOV1 in NPC and its correlation with clinicopathological features of patients.

Methods: Western blotting and real-time PCR were conducted to examine PTOV1 expression levels in NPC cell lines and biopsy tissues compared with normal controls. Immunohistochemistry (IHC) was performed to analyze PTOV1 protein expression in paraffin-embedded tissues from 123 patients. Statistical analyses were applied to evaluate the clinical significance of PTOV1 expression.

Results: PTOV1 mRNA and protein levels were upregulated in NPC cell lines and clinical samples. IHC analyses showed that PTOV1 was highly expressed in 68 (55.3%) of 123 NPC specimens. Statistical analysis revealed that PTOV1 expression was significantly correlated with clinical stage (P < 0.001), T classification (P = 0.042) and N classification (P = 0.001). Patients with a higher PTOV1 expression had shorter overall survival compared with those with a lower PTOV1 expression level, especially in lower N stage patients. Multivariate analyses suggested that PTOV1 expression was an independent prognostic marker for survival in NPC patients.

Conclusions: Our data demonstrated that PTOV1 overexpression is associated with poor survival outcomes of NPC patients, especially in N0-1 patients. Hence, PTOV1 may help to detect early lymph node metastasis of NPC patients and serve as an independent prognostic biomarker for human NPC.

No MeSH data available.


Related in: MedlinePlus

Overexpression of PTOV1 protein and mRNA in NPC tissues.(A) Expression of PTOV1 protein in three normal nasopharyngeal epithelial tissues and eight NPC tissues, measured by western blotting. GAPDH was used as a loading control. (B) Real-time PCR analysis of PTOV1 mRNA in three normal nasopharyngeal epithelial tissues and eight NPC tissues. Expression data were normalized to GAPDH. Error bars represent the SD, calculated from three parallel experiments. * P < 0.05.
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pone.0136448.g002: Overexpression of PTOV1 protein and mRNA in NPC tissues.(A) Expression of PTOV1 protein in three normal nasopharyngeal epithelial tissues and eight NPC tissues, measured by western blotting. GAPDH was used as a loading control. (B) Real-time PCR analysis of PTOV1 mRNA in three normal nasopharyngeal epithelial tissues and eight NPC tissues. Expression data were normalized to GAPDH. Error bars represent the SD, calculated from three parallel experiments. * P < 0.05.

Mentions: As PTOV1 is overexpressed in NPC cell lines, we investigated PTOV1 expression in NPC biopsies. The expression of PTOV1 protein was overexpressed in all eight NPC samples, and was barely detectable in three normal nasopharyngeal epithelial tissue samples (Fig 2A). Consistently, real-time PCR analysis revealed that PTOV1 mRNA was upregulated in tumor samples (Fig 2B), further confirming that PTOV1 is overexpressed in NPC tissues.


Prostate Tumor Overexpressed 1 (PTOV1) Is a Novel Prognostic Marker for Nasopharyngeal Carcinoma Progression and Poor Survival Outcomes.

Yang Q, Lin H, Wu S, Lei F, Zhu X, Song L, Hong M, Guo L - PLoS ONE (2015)

Overexpression of PTOV1 protein and mRNA in NPC tissues.(A) Expression of PTOV1 protein in three normal nasopharyngeal epithelial tissues and eight NPC tissues, measured by western blotting. GAPDH was used as a loading control. (B) Real-time PCR analysis of PTOV1 mRNA in three normal nasopharyngeal epithelial tissues and eight NPC tissues. Expression data were normalized to GAPDH. Error bars represent the SD, calculated from three parallel experiments. * P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4549246&req=5

pone.0136448.g002: Overexpression of PTOV1 protein and mRNA in NPC tissues.(A) Expression of PTOV1 protein in three normal nasopharyngeal epithelial tissues and eight NPC tissues, measured by western blotting. GAPDH was used as a loading control. (B) Real-time PCR analysis of PTOV1 mRNA in three normal nasopharyngeal epithelial tissues and eight NPC tissues. Expression data were normalized to GAPDH. Error bars represent the SD, calculated from three parallel experiments. * P < 0.05.
Mentions: As PTOV1 is overexpressed in NPC cell lines, we investigated PTOV1 expression in NPC biopsies. The expression of PTOV1 protein was overexpressed in all eight NPC samples, and was barely detectable in three normal nasopharyngeal epithelial tissue samples (Fig 2A). Consistently, real-time PCR analysis revealed that PTOV1 mRNA was upregulated in tumor samples (Fig 2B), further confirming that PTOV1 is overexpressed in NPC tissues.

Bottom Line: Patients with a higher PTOV1 expression had shorter overall survival compared with those with a lower PTOV1 expression level, especially in lower N stage patients.Our data demonstrated that PTOV1 overexpression is associated with poor survival outcomes of NPC patients, especially in N0-1 patients.Hence, PTOV1 may help to detect early lymph node metastasis of NPC patients and serve as an independent prognostic biomarker for human NPC.

View Article: PubMed Central - PubMed

Affiliation: Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, P.R. China; Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, Guangzhou, 510060, P.R. China.

ABSTRACT

Background: Prostate tumor overexpressed 1 (PTOV1) has been reported to contribute to increased cancer proliferation. However, the clinical significance of PTOV1 in the development and progression of nasopharyngeal carcinoma (NPC) is unclear. Our study aimed to investigate the expression pattern of PTOV1 in NPC and its correlation with clinicopathological features of patients.

Methods: Western blotting and real-time PCR were conducted to examine PTOV1 expression levels in NPC cell lines and biopsy tissues compared with normal controls. Immunohistochemistry (IHC) was performed to analyze PTOV1 protein expression in paraffin-embedded tissues from 123 patients. Statistical analyses were applied to evaluate the clinical significance of PTOV1 expression.

Results: PTOV1 mRNA and protein levels were upregulated in NPC cell lines and clinical samples. IHC analyses showed that PTOV1 was highly expressed in 68 (55.3%) of 123 NPC specimens. Statistical analysis revealed that PTOV1 expression was significantly correlated with clinical stage (P < 0.001), T classification (P = 0.042) and N classification (P = 0.001). Patients with a higher PTOV1 expression had shorter overall survival compared with those with a lower PTOV1 expression level, especially in lower N stage patients. Multivariate analyses suggested that PTOV1 expression was an independent prognostic marker for survival in NPC patients.

Conclusions: Our data demonstrated that PTOV1 overexpression is associated with poor survival outcomes of NPC patients, especially in N0-1 patients. Hence, PTOV1 may help to detect early lymph node metastasis of NPC patients and serve as an independent prognostic biomarker for human NPC.

No MeSH data available.


Related in: MedlinePlus