Limits...
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.

Oliver RA, Lovric V, Yu Y, Christou C, Aiken SS, Cooper JJ, Walsh WR - PLoS ONE (2015)

Bottom Line: Complete resorption was confirmed radiographically at 3 weeks post-implantation.Serological inflammatory cytokine expression for IL-6, TNF-α and IL-1β indicated no unusual inflammatory response to the implanted materials or surgical procedure.The two materials tested were effective in resolving the surgically created dead space, and did not elicit any unexpected adverse host response.

View Article: PubMed Central - PubMed

Affiliation: Surgical & Orthopaedic Research Laboratories, University of New South Wales Australia, Sydney, Australia, Prince of Wales Clinical School, Prince of Wales Hospital, Randwick, NSW, Australia.

ABSTRACT
Following extensive surgical debridement in the treatment of infection, a "dead space" can result following surgical closure that can fill with hematoma, an environment conducive to bacterial growth. The eradication of dead space is essential in order to prevent recurrent infection. This study describes a novel small animal model to investigate dead-space management in muscle tissue. Two absorbable test materials were implanted in each animal; beads of calcium sulfate alone, and beads loaded with vancomycin and tobramycin. In-life blood samples and radiographs were taken from each animal following implantation. Animals were sacrificed at 1, 7, 21, 42, and 63 days post-operatively (n = 4), and implant sites were analysed by micro-computed tomography, histology and immunohistochemistry. Complete resorption was confirmed radiographically at 3 weeks post-implantation. Histologically, the host tissue response to both materials was identical, and subsequent healing at the implant sites was observed with no dead space remaining. Vancomycin was not detected in blood serum. However, peak tobramycin levels were detected in all animals at 6 hours post-implantation with no detectable levels in any animals at 72 hours post implantation. Serological inflammatory cytokine expression for IL-6, TNF-α and IL-1β indicated no unusual inflammatory response to the implanted materials or surgical procedure. The model was found to be convenient and effective for the assessment of implant materials for management of dead space in muscle tissue. The two materials tested were effective in resolving the surgically created dead space, and did not elicit any unexpected adverse host response.

No MeSH data available.


Related in: MedlinePlus

Graphical representation of antibiotic levels within beads.Measured vancomycin and tobramycin levels within the remnant beads found at the implantation site at 24 hours and day 7.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4549236&req=5

pone.0136514.g008: Graphical representation of antibiotic levels within beads.Measured vancomycin and tobramycin levels within the remnant beads found at the implantation site at 24 hours and day 7.

Mentions: During harvest the surgical sites were carefully dissected and examined for the presence of any residual calcium sulfate beads. A portion (0.1g) of any residual calcium sulfate bead present was harvested. Residual beads were only found in animals allocated for 24 hours and day 7 time points. Vancomycin and tobramycin levels in the samples per gram of material (CSVT) were determined. Results are represented in Fig 8.


Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.

Oliver RA, Lovric V, Yu Y, Christou C, Aiken SS, Cooper JJ, Walsh WR - PLoS ONE (2015)

Graphical representation of antibiotic levels within beads.Measured vancomycin and tobramycin levels within the remnant beads found at the implantation site at 24 hours and day 7.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549236&req=5

pone.0136514.g008: Graphical representation of antibiotic levels within beads.Measured vancomycin and tobramycin levels within the remnant beads found at the implantation site at 24 hours and day 7.
Mentions: During harvest the surgical sites were carefully dissected and examined for the presence of any residual calcium sulfate beads. A portion (0.1g) of any residual calcium sulfate bead present was harvested. Residual beads were only found in animals allocated for 24 hours and day 7 time points. Vancomycin and tobramycin levels in the samples per gram of material (CSVT) were determined. Results are represented in Fig 8.

Bottom Line: Complete resorption was confirmed radiographically at 3 weeks post-implantation.Serological inflammatory cytokine expression for IL-6, TNF-α and IL-1β indicated no unusual inflammatory response to the implanted materials or surgical procedure.The two materials tested were effective in resolving the surgically created dead space, and did not elicit any unexpected adverse host response.

View Article: PubMed Central - PubMed

Affiliation: Surgical & Orthopaedic Research Laboratories, University of New South Wales Australia, Sydney, Australia, Prince of Wales Clinical School, Prince of Wales Hospital, Randwick, NSW, Australia.

ABSTRACT
Following extensive surgical debridement in the treatment of infection, a "dead space" can result following surgical closure that can fill with hematoma, an environment conducive to bacterial growth. The eradication of dead space is essential in order to prevent recurrent infection. This study describes a novel small animal model to investigate dead-space management in muscle tissue. Two absorbable test materials were implanted in each animal; beads of calcium sulfate alone, and beads loaded with vancomycin and tobramycin. In-life blood samples and radiographs were taken from each animal following implantation. Animals were sacrificed at 1, 7, 21, 42, and 63 days post-operatively (n = 4), and implant sites were analysed by micro-computed tomography, histology and immunohistochemistry. Complete resorption was confirmed radiographically at 3 weeks post-implantation. Histologically, the host tissue response to both materials was identical, and subsequent healing at the implant sites was observed with no dead space remaining. Vancomycin was not detected in blood serum. However, peak tobramycin levels were detected in all animals at 6 hours post-implantation with no detectable levels in any animals at 72 hours post implantation. Serological inflammatory cytokine expression for IL-6, TNF-α and IL-1β indicated no unusual inflammatory response to the implanted materials or surgical procedure. The model was found to be convenient and effective for the assessment of implant materials for management of dead space in muscle tissue. The two materials tested were effective in resolving the surgically created dead space, and did not elicit any unexpected adverse host response.

No MeSH data available.


Related in: MedlinePlus