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Effects of direct current electric-field using ITO plate on breast cancer cell migration.

Kim MS, Lee MH, Kwon BJ, Seo HJ, Koo MA, You KE, Kim D, Park JC - Biomater Res (2014)

Bottom Line: The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm.However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea.

ABSTRACT

Background: Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated.

Results: Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.

Conclusions: In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

No MeSH data available.


Related in: MedlinePlus

Real time observation system. Schematic diagram of the real time observation system for the evaluation of cell migration.
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Fig2: Real time observation system. Schematic diagram of the real time observation system for the evaluation of cell migration.

Mentions: We used the real time observation system (Figure 2) to observe the migration of the cells on the ITO plate. The real time observation system consisted of incubator system installed with the microscope to observe live cells migration. The incubator was regulated by temperature and gas composition controlling program (CCP ver. 3.8, Live Cell Instrument, Korea) under proper environment for cell (CO2 5%, 37°C).Figure 2


Effects of direct current electric-field using ITO plate on breast cancer cell migration.

Kim MS, Lee MH, Kwon BJ, Seo HJ, Koo MA, You KE, Kim D, Park JC - Biomater Res (2014)

Real time observation system. Schematic diagram of the real time observation system for the evaluation of cell migration.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549139&req=5

Fig2: Real time observation system. Schematic diagram of the real time observation system for the evaluation of cell migration.
Mentions: We used the real time observation system (Figure 2) to observe the migration of the cells on the ITO plate. The real time observation system consisted of incubator system installed with the microscope to observe live cells migration. The incubator was regulated by temperature and gas composition controlling program (CCP ver. 3.8, Live Cell Instrument, Korea) under proper environment for cell (CO2 5%, 37°C).Figure 2

Bottom Line: The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm.However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea.

ABSTRACT

Background: Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated.

Results: Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.

Conclusions: In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

No MeSH data available.


Related in: MedlinePlus