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Effects of direct current electric-field using ITO plate on breast cancer cell migration.

Kim MS, Lee MH, Kwon BJ, Seo HJ, Koo MA, You KE, Kim D, Park JC - Biomater Res (2014)

Bottom Line: The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm.However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea.

ABSTRACT

Background: Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated.

Results: Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.

Conclusions: In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

No MeSH data available.


Related in: MedlinePlus

Indium-tin-oxide (ITO) plate electrotaxis system. Indium-tin-oxide (ITO) plate electrotaxis system (A) and Schematic diagram of electrotaxis using ITO plate (B).
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Fig1: Indium-tin-oxide (ITO) plate electrotaxis system. Indium-tin-oxide (ITO) plate electrotaxis system (A) and Schematic diagram of electrotaxis using ITO plate (B).

Mentions: Electrotaxis system using Indium-Tin-Oxide coated glasses were designed for currents contact cells directly. Indium-Tin-oxide (ITO) coated glasses were kindly provided by Kwangwoon University and were cut with approximately dimensions 2 × 2 cm2. Cu tape (3 M, USA) connected both ends of 2 × 2 cm2 cut ITO coated glasses (ITO plate) with 5 cm long electric wire, then the ITO plate was dipped into 70% ethanol for 30 minutes. The sterilized grease were pasted to one side of the silicon culture insert (Ibidi, Munchen, Germany), then put the silicon insert on the ITO coated glass surface (Figure 1A).Figure 1


Effects of direct current electric-field using ITO plate on breast cancer cell migration.

Kim MS, Lee MH, Kwon BJ, Seo HJ, Koo MA, You KE, Kim D, Park JC - Biomater Res (2014)

Indium-tin-oxide (ITO) plate electrotaxis system. Indium-tin-oxide (ITO) plate electrotaxis system (A) and Schematic diagram of electrotaxis using ITO plate (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549139&req=5

Fig1: Indium-tin-oxide (ITO) plate electrotaxis system. Indium-tin-oxide (ITO) plate electrotaxis system (A) and Schematic diagram of electrotaxis using ITO plate (B).
Mentions: Electrotaxis system using Indium-Tin-Oxide coated glasses were designed for currents contact cells directly. Indium-Tin-oxide (ITO) coated glasses were kindly provided by Kwangwoon University and were cut with approximately dimensions 2 × 2 cm2. Cu tape (3 M, USA) connected both ends of 2 × 2 cm2 cut ITO coated glasses (ITO plate) with 5 cm long electric wire, then the ITO plate was dipped into 70% ethanol for 30 minutes. The sterilized grease were pasted to one side of the silicon culture insert (Ibidi, Munchen, Germany), then put the silicon insert on the ITO coated glass surface (Figure 1A).Figure 1

Bottom Line: The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm.However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752 Korea.

ABSTRACT

Background: Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated.

Results: Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was -0.046 ± 0.357 under the 0 V/cm and was 0.273 ± 0.231 under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate.

Conclusions: In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

No MeSH data available.


Related in: MedlinePlus