Limits...
Effect of osteoconductive hyaluronate hydrogels on calvarial bone regeneration.

Yeom J, Hwang BW, Yang DJ, Shin HI, Hahn SK - Biomater Res (2014)

Bottom Line: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL).In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784 Korea.

ABSTRACT

Background: Without exploitation of possibly immunogenic and carcinogenic bone morphogenetic protein, we developed simple but clinically feasible artificial bone graft using osteoconductive hyaluronate (HA) hydrogels and bioactive MegaGen synthetic bone (MGSB).

Methods: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. The bone regeneration by the MGSB/HA-GEL hydrogel complex was assessed in the skull of New Zealand white male rabbits in 4 and 8 weeks.

Results: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA. In addition, histological analysis of dissected tissues with hematoxylin and eosin staining confirmed the effective in vivo bone regeneration by the MGSB/HA-GEL hydrogel complex. The MGSB/HA-GEL hydrogels were well resorbed and partially substituted to the lamellar bone after implantation for 8 weeks.

Conclusions: The novel artificial bone graft of MGSB/HA-GEL hydrogel complex for effective bone regeneration might be clinically feasible for further development.

No MeSH data available.


Photomicrographs if the calvarial critical-sized bone defects in New Zealand white rabbits after bone regeneration for 4 weeks: (A)No treatment,(B)MGSB only,(C)MGSB/HA, and(D)MGSB/HA-GEL hydrogel.(E) MGSB/HA-GEL hydrogel after bone regeneration for 8 weeks. Scale bars: left, 1000 μm; right, 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4549130&req=5

Fig5: Photomicrographs if the calvarial critical-sized bone defects in New Zealand white rabbits after bone regeneration for 4 weeks: (A)No treatment,(B)MGSB only,(C)MGSB/HA, and(D)MGSB/HA-GEL hydrogel.(E) MGSB/HA-GEL hydrogel after bone regeneration for 8 weeks. Scale bars: left, 1000 μm; right, 200 μm.

Mentions: The calvarial critical sized bone defects were formed in the New Zealand white rabbits to assess the effect of PBS, MGSB only, MGSB/HA solution, and MGSB/HA-GEL hydrogel on the bone regeneration (Figure 5). We could confirm the effective bone regeneration by the osteoconductive HA (Figure 5B and C). The regenerated bone by MGSB/HA was well matured around the MGSB, whereas that by MGSB only was partially segregated. Figure 5D shows the more effective bone regeneration by MGSB/HA-GEL hydrogels than those by the control samples. The newly formed bone was well interconnected to the MGSB after bone regeneration for 4 weeks. Remarkably, as shown in Figure 5E, MGSB was partially degraded and substituted to the lamellar bone structure after bone regeneration for 8 weeks. It was thought that osteoblast and MSC were recruited and proliferated at the initial bone regeneration, followed by the sequential replacement with the lamellar bone.Figure 4


Effect of osteoconductive hyaluronate hydrogels on calvarial bone regeneration.

Yeom J, Hwang BW, Yang DJ, Shin HI, Hahn SK - Biomater Res (2014)

Photomicrographs if the calvarial critical-sized bone defects in New Zealand white rabbits after bone regeneration for 4 weeks: (A)No treatment,(B)MGSB only,(C)MGSB/HA, and(D)MGSB/HA-GEL hydrogel.(E) MGSB/HA-GEL hydrogel after bone regeneration for 8 weeks. Scale bars: left, 1000 μm; right, 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549130&req=5

Fig5: Photomicrographs if the calvarial critical-sized bone defects in New Zealand white rabbits after bone regeneration for 4 weeks: (A)No treatment,(B)MGSB only,(C)MGSB/HA, and(D)MGSB/HA-GEL hydrogel.(E) MGSB/HA-GEL hydrogel after bone regeneration for 8 weeks. Scale bars: left, 1000 μm; right, 200 μm.
Mentions: The calvarial critical sized bone defects were formed in the New Zealand white rabbits to assess the effect of PBS, MGSB only, MGSB/HA solution, and MGSB/HA-GEL hydrogel on the bone regeneration (Figure 5). We could confirm the effective bone regeneration by the osteoconductive HA (Figure 5B and C). The regenerated bone by MGSB/HA was well matured around the MGSB, whereas that by MGSB only was partially segregated. Figure 5D shows the more effective bone regeneration by MGSB/HA-GEL hydrogels than those by the control samples. The newly formed bone was well interconnected to the MGSB after bone regeneration for 4 weeks. Remarkably, as shown in Figure 5E, MGSB was partially degraded and substituted to the lamellar bone structure after bone regeneration for 8 weeks. It was thought that osteoblast and MSC were recruited and proliferated at the initial bone regeneration, followed by the sequential replacement with the lamellar bone.Figure 4

Bottom Line: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL).In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784 Korea.

ABSTRACT

Background: Without exploitation of possibly immunogenic and carcinogenic bone morphogenetic protein, we developed simple but clinically feasible artificial bone graft using osteoconductive hyaluronate (HA) hydrogels and bioactive MegaGen synthetic bone (MGSB).

Methods: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. The bone regeneration by the MGSB/HA-GEL hydrogel complex was assessed in the skull of New Zealand white male rabbits in 4 and 8 weeks.

Results: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA. In addition, histological analysis of dissected tissues with hematoxylin and eosin staining confirmed the effective in vivo bone regeneration by the MGSB/HA-GEL hydrogel complex. The MGSB/HA-GEL hydrogels were well resorbed and partially substituted to the lamellar bone after implantation for 8 weeks.

Conclusions: The novel artificial bone graft of MGSB/HA-GEL hydrogel complex for effective bone regeneration might be clinically feasible for further development.

No MeSH data available.