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Purification and biocompatibility of fermented hyaluronic acid for its applications to biomaterials.

Choi S, Choi W, Kim S, Lee SY, Noh I, Kim CW - Biomater Res (2014)

Bottom Line: While low molecular weight impurities and insoluble impurities were successfully removed using a ultrafiltration membrane with 50 KDa molecular weight cut, endotoxins, high molecular weight proteins and nucleic acids were removed from the broth by employing adsorbents such as alumina and activated carbons.Alumina showed the best results for the removal of endotoxins, all of the activated carbons were very effective in the removal of high molecular weight proteins and nucleic acids.We obtained high molecular weight HA with excellent biocompatibility as judged by both measurement of cell proliferation and viability, indicating high possibility of its applications to biomaterials.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Biotechnology, Korea University, Seoul, 136-701 Republic of Korea ; Department of Bioplant, Hanmi Pharm. Co, Pyeongtaek, 451-805 Korea.

ABSTRACT

Background: Hyaluronic acid (HA) is of importance due to its diverse applications in pharmaceuticals and medical devices such as dermal filler, adhesion barriers, carrier for cells and bioactive molecules as well as scaffold biomaterials for tissue engineering. Evaluations of purification and biocompatibility of HA are required for its applications to biomaterials.

Results: After synthesizing HA by fermentation of streptococcus zooepidemicus for 25 hr, extensively purification of the fermented broth was performed to remove impurities using a filtration process for insoluble components and cells, and diverse adsorbents for soluble impurities. Its in vitro biocompatibility has been evaluated by measurement of cell counting and assay of cell live and dead. 60% yield of white HA powder was obtained, having 15-17 dL/g intrinsic viscosity with a molecular weight of approximately 1,000 kDa. While low molecular weight impurities and insoluble impurities were successfully removed using a ultrafiltration membrane with 50 KDa molecular weight cut, endotoxins, high molecular weight proteins and nucleic acids were removed from the broth by employing adsorbents such as alumina and activated carbons. Alumina showed the best results for the removal of endotoxins, all of the activated carbons were very effective in the removal of high molecular weight proteins and nucleic acids. The purified HA solution showed excellent cell compatibility with no cell damages as observed by both measurement of cell proliferation and observation of cell viability.

Conclusions: We obtained high molecular weight HA with excellent biocompatibility as judged by both measurement of cell proliferation and viability, indicating high possibility of its applications to biomaterials.

No MeSH data available.


Related in: MedlinePlus

Schematic processes of bacterial fermentation and HA separations and characterizations.
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Fig1: Schematic processes of bacterial fermentation and HA separations and characterizations.

Mentions: Recently demands for HA products from bacterial fermentation have significantly increased because of both their increased uses as medical devices and the immune issues that occurred from the use of animal based HA. Due to the both high price of HA and the high standard requirements of its applications in medical products, high quality HA products rather than high quantity have been the primary criteria used when selecting the bacterial strains used for HA production and the methods of HA purification. In this study, we examined the effects of various adsorbents such as activated carbons and alumina on the purification of fermented HA broth as well as biocompatibility tests. Schematic processes of bacterial fermentation and HA separations and characterizations are shown in Figure 1. The HA obtained in this study may expand the potential application of HA in biomaterials targeting on the areas of cell therapy, tissue engineering and medical devices.Figure 1


Purification and biocompatibility of fermented hyaluronic acid for its applications to biomaterials.

Choi S, Choi W, Kim S, Lee SY, Noh I, Kim CW - Biomater Res (2014)

Schematic processes of bacterial fermentation and HA separations and characterizations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549129&req=5

Fig1: Schematic processes of bacterial fermentation and HA separations and characterizations.
Mentions: Recently demands for HA products from bacterial fermentation have significantly increased because of both their increased uses as medical devices and the immune issues that occurred from the use of animal based HA. Due to the both high price of HA and the high standard requirements of its applications in medical products, high quality HA products rather than high quantity have been the primary criteria used when selecting the bacterial strains used for HA production and the methods of HA purification. In this study, we examined the effects of various adsorbents such as activated carbons and alumina on the purification of fermented HA broth as well as biocompatibility tests. Schematic processes of bacterial fermentation and HA separations and characterizations are shown in Figure 1. The HA obtained in this study may expand the potential application of HA in biomaterials targeting on the areas of cell therapy, tissue engineering and medical devices.Figure 1

Bottom Line: While low molecular weight impurities and insoluble impurities were successfully removed using a ultrafiltration membrane with 50 KDa molecular weight cut, endotoxins, high molecular weight proteins and nucleic acids were removed from the broth by employing adsorbents such as alumina and activated carbons.Alumina showed the best results for the removal of endotoxins, all of the activated carbons were very effective in the removal of high molecular weight proteins and nucleic acids.We obtained high molecular weight HA with excellent biocompatibility as judged by both measurement of cell proliferation and viability, indicating high possibility of its applications to biomaterials.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Biotechnology, Korea University, Seoul, 136-701 Republic of Korea ; Department of Bioplant, Hanmi Pharm. Co, Pyeongtaek, 451-805 Korea.

ABSTRACT

Background: Hyaluronic acid (HA) is of importance due to its diverse applications in pharmaceuticals and medical devices such as dermal filler, adhesion barriers, carrier for cells and bioactive molecules as well as scaffold biomaterials for tissue engineering. Evaluations of purification and biocompatibility of HA are required for its applications to biomaterials.

Results: After synthesizing HA by fermentation of streptococcus zooepidemicus for 25 hr, extensively purification of the fermented broth was performed to remove impurities using a filtration process for insoluble components and cells, and diverse adsorbents for soluble impurities. Its in vitro biocompatibility has been evaluated by measurement of cell counting and assay of cell live and dead. 60% yield of white HA powder was obtained, having 15-17 dL/g intrinsic viscosity with a molecular weight of approximately 1,000 kDa. While low molecular weight impurities and insoluble impurities were successfully removed using a ultrafiltration membrane with 50 KDa molecular weight cut, endotoxins, high molecular weight proteins and nucleic acids were removed from the broth by employing adsorbents such as alumina and activated carbons. Alumina showed the best results for the removal of endotoxins, all of the activated carbons were very effective in the removal of high molecular weight proteins and nucleic acids. The purified HA solution showed excellent cell compatibility with no cell damages as observed by both measurement of cell proliferation and observation of cell viability.

Conclusions: We obtained high molecular weight HA with excellent biocompatibility as judged by both measurement of cell proliferation and viability, indicating high possibility of its applications to biomaterials.

No MeSH data available.


Related in: MedlinePlus