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Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression.

Borghetti G, Yamaguchi AA, Aikawa J, Yamazaki RK, de Brito GA, Fernandes LC - Lipids Health Dis (2015)

Bottom Line: Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells.Protein expression was done by western blotting in Walker 256 tumor tissue samples.Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.

View Article: PubMed Central - PubMed

Affiliation: Centro de Estudos da Biodiversidade, Universidade Federal de Roraima, Campus Paricarana Avenida Capitão Ene Garcez, 2413, Bairro Aeroporto Cep: 69310-000, Boa Vista, Roraima, Brazil. borghettigina@hotmail.com.

ABSTRACT

Background: Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells. Nevertheless, few in vivo experiments have provided data of its ability on apoptosis protein expression in tumor tissue. Thus, in this study we investigate the effect of FO supplementation on apoptosis protein expression in Walker 256 tumor bearing rats. Male Wistar rats were randomly assigned to three groups: fed with regular chow (W); fed regular chow supplemented with FO (WFO) or coconut fat (WCO) (1 g/kg body weight/daily). After thirty days, all animals were inoculated subcutaneously with Walker 256 tumor cells.

Findings: Protein expression was done by western blotting in Walker 256 tumor tissue samples. FO decreased the Bcl-2/Bax ratio (p < 0.05) and increased the p53 (p < 0.05), cleaved caspase-7 (p < 0.05) and cleaved caspase-3 (p < 0.05) in Walker 256 tumor tissue.

Conclusions: Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.

No MeSH data available.


Related in: MedlinePlus

Protein expression of PAPR-1 in the Walker 256 tumor tissue. Anti-β-actin was used as a loading control to normalize the data. Data are mean ± SEM (n = 7) of Walker 256 tumor-bearing rats (W), Walker 256 tumor-bearing rats supplemented with fish oil (WFO) and coconut fat (WCO) * p < 0.05 vs. W and WCO group
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Fig6: Protein expression of PAPR-1 in the Walker 256 tumor tissue. Anti-β-actin was used as a loading control to normalize the data. Data are mean ± SEM (n = 7) of Walker 256 tumor-bearing rats (W), Walker 256 tumor-bearing rats supplemented with fish oil (WFO) and coconut fat (WCO) * p < 0.05 vs. W and WCO group

Mentions: FO supplementation decreased the n-6/n-3 PUFA ratio in tumor tissue by 2 fold (Table 1) and reduced the tumor weight by 47 % (W 16.9 ± 1.2 vs. WFO 9.0 ± 0.8 vs. WCO 17.1 ± 2.1) (Fig. 1) when compared with Walker 256 tumor-bearing rats fed with regular chow group (W) or WCO group (p < 0.05). Tumors from Walker 256 tumor-bearing rats fed with regular chow plus fish oil supplementation group (WFO) had a significant decrease of 20.3 % (W 1.13 ± 0.03 vs. WFO 0.90 ± 0.03 vs. WCO 1.09 ± 0.03) from Bcl-2/Bax ratio when compared with W or WCO group (p < 0.05) (Fig. 2). FO supplementation also increased the protein expression of p53 by 29 % (W 0.86 ± 0.04 vs. WFO 1.11 ± 0.04 vs. WCO 0.91. ± 0.04) (p < 0.05) (Fig. 3), cleaved caspase-7 by 21.4 % (W 0.98 ± 0.01 vs. WFO 1.19 ± 0.03 vs. WCO 0.99 ± 0.03) (p < 0.05) (Fig. 4) and cleaved caspase-3 by 26 % (W 0.92 ± 0.02 vs. WOP 1.16 ± 0.04 vs. WCO 0.94 ± 0.02) (p < 0.05) (Fig. 5) in tumor tissue when compared with W and WCO. FO supplementation did not modify the PARP-1 protein expression in tumor tissue (W 1.03 ± 0.02 vs. WFO 1.08 ± 0.03 vs. WCO 1.04 ± 0.05) (Fig. 6). There was no difference in the tumor protein expression of WCO when compared with W group (p > 0.05).Fig. 1


Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression.

Borghetti G, Yamaguchi AA, Aikawa J, Yamazaki RK, de Brito GA, Fernandes LC - Lipids Health Dis (2015)

Protein expression of PAPR-1 in the Walker 256 tumor tissue. Anti-β-actin was used as a loading control to normalize the data. Data are mean ± SEM (n = 7) of Walker 256 tumor-bearing rats (W), Walker 256 tumor-bearing rats supplemented with fish oil (WFO) and coconut fat (WCO) * p < 0.05 vs. W and WCO group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549106&req=5

Fig6: Protein expression of PAPR-1 in the Walker 256 tumor tissue. Anti-β-actin was used as a loading control to normalize the data. Data are mean ± SEM (n = 7) of Walker 256 tumor-bearing rats (W), Walker 256 tumor-bearing rats supplemented with fish oil (WFO) and coconut fat (WCO) * p < 0.05 vs. W and WCO group
Mentions: FO supplementation decreased the n-6/n-3 PUFA ratio in tumor tissue by 2 fold (Table 1) and reduced the tumor weight by 47 % (W 16.9 ± 1.2 vs. WFO 9.0 ± 0.8 vs. WCO 17.1 ± 2.1) (Fig. 1) when compared with Walker 256 tumor-bearing rats fed with regular chow group (W) or WCO group (p < 0.05). Tumors from Walker 256 tumor-bearing rats fed with regular chow plus fish oil supplementation group (WFO) had a significant decrease of 20.3 % (W 1.13 ± 0.03 vs. WFO 0.90 ± 0.03 vs. WCO 1.09 ± 0.03) from Bcl-2/Bax ratio when compared with W or WCO group (p < 0.05) (Fig. 2). FO supplementation also increased the protein expression of p53 by 29 % (W 0.86 ± 0.04 vs. WFO 1.11 ± 0.04 vs. WCO 0.91. ± 0.04) (p < 0.05) (Fig. 3), cleaved caspase-7 by 21.4 % (W 0.98 ± 0.01 vs. WFO 1.19 ± 0.03 vs. WCO 0.99 ± 0.03) (p < 0.05) (Fig. 4) and cleaved caspase-3 by 26 % (W 0.92 ± 0.02 vs. WOP 1.16 ± 0.04 vs. WCO 0.94 ± 0.02) (p < 0.05) (Fig. 5) in tumor tissue when compared with W and WCO. FO supplementation did not modify the PARP-1 protein expression in tumor tissue (W 1.03 ± 0.02 vs. WFO 1.08 ± 0.03 vs. WCO 1.04 ± 0.05) (Fig. 6). There was no difference in the tumor protein expression of WCO when compared with W group (p > 0.05).Fig. 1

Bottom Line: Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells.Protein expression was done by western blotting in Walker 256 tumor tissue samples.Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.

View Article: PubMed Central - PubMed

Affiliation: Centro de Estudos da Biodiversidade, Universidade Federal de Roraima, Campus Paricarana Avenida Capitão Ene Garcez, 2413, Bairro Aeroporto Cep: 69310-000, Boa Vista, Roraima, Brazil. borghettigina@hotmail.com.

ABSTRACT

Background: Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells. Nevertheless, few in vivo experiments have provided data of its ability on apoptosis protein expression in tumor tissue. Thus, in this study we investigate the effect of FO supplementation on apoptosis protein expression in Walker 256 tumor bearing rats. Male Wistar rats were randomly assigned to three groups: fed with regular chow (W); fed regular chow supplemented with FO (WFO) or coconut fat (WCO) (1 g/kg body weight/daily). After thirty days, all animals were inoculated subcutaneously with Walker 256 tumor cells.

Findings: Protein expression was done by western blotting in Walker 256 tumor tissue samples. FO decreased the Bcl-2/Bax ratio (p < 0.05) and increased the p53 (p < 0.05), cleaved caspase-7 (p < 0.05) and cleaved caspase-3 (p < 0.05) in Walker 256 tumor tissue.

Conclusions: Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.

No MeSH data available.


Related in: MedlinePlus