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Antitumoral potency of methanolic extract from Nitraria retusa leaves via its immunomodulatory effect.

Boubaker J, Chaabane F, Bedoui A, Aloui R, Ahmed BB, Ghedira K, Chekir-Ghedira L - Cancer Cell Int. (2015)

Bottom Line: It also promoted lysosomal activity of treated animal macrophages.Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Higher Institute of Medical Technologies of Tunis, Tunis El Manar University, Tunis Rommana, 1068 Tunisia.

ABSTRACT

Background: The purpose of this study was to assess the antitumoral effect of the methanol extract (MeOH) from Nitraria retusa leaves and to investigate its immunomodulatory activity that mediated the prevention of tumor progression in tumor-bearing mice.

Methods: Balb/c mice weighing 18-20 g were subcutaneously implanted with B16-F10 cells then injected intra-peritoneally, 7 days later with (200 mg/kg bw) of MeOH extract, for 21 days. After euthanization on day 21, the tumors were weighed. Lymphocyte proliferation, cytotoxic T lymphocyte (CTL) and NK activity were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring their lysosomal activity and nitric oxide production.

Results: The methanol extract inhibited significantly the growth of the implanted tumor, and increased remarkably splenocyte proliferation as well as NK and CTL activities, in tumor-bearing mice. It also promoted lysosomal activity of treated animal macrophages.

Conclusion: Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.

No MeSH data available.


Related in: MedlinePlus

Effect of methanol extract from N. retusa on NK cell activity in vivo. All data shown are mean (±SD)  % NK activity, and are representative of two independent experiments (n = 10 mice per group/time point). *P < 0.05 significantly different from that of the control mice (NC). (a) P < 0.05 significantly different from that of mice with melanoma (PC)
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Fig5: Effect of methanol extract from N. retusa on NK cell activity in vivo. All data shown are mean (±SD)  % NK activity, and are representative of two independent experiments (n = 10 mice per group/time point). *P < 0.05 significantly different from that of the control mice (NC). (a) P < 0.05 significantly different from that of mice with melanoma (PC)

Mentions: The results of this assay were reported in Fig. 5. In fact, NK activity increases from 21.89 % in healthy mice to 48.66 % in the tumor-bearing (PC) mice. Treatment with (MeOH) extract increased the NK activity by 136.27 % in tumor-injected mice, revealing thus an induction of this cell defense system.Fig. 5


Antitumoral potency of methanolic extract from Nitraria retusa leaves via its immunomodulatory effect.

Boubaker J, Chaabane F, Bedoui A, Aloui R, Ahmed BB, Ghedira K, Chekir-Ghedira L - Cancer Cell Int. (2015)

Effect of methanol extract from N. retusa on NK cell activity in vivo. All data shown are mean (±SD)  % NK activity, and are representative of two independent experiments (n = 10 mice per group/time point). *P < 0.05 significantly different from that of the control mice (NC). (a) P < 0.05 significantly different from that of mice with melanoma (PC)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549073&req=5

Fig5: Effect of methanol extract from N. retusa on NK cell activity in vivo. All data shown are mean (±SD)  % NK activity, and are representative of two independent experiments (n = 10 mice per group/time point). *P < 0.05 significantly different from that of the control mice (NC). (a) P < 0.05 significantly different from that of mice with melanoma (PC)
Mentions: The results of this assay were reported in Fig. 5. In fact, NK activity increases from 21.89 % in healthy mice to 48.66 % in the tumor-bearing (PC) mice. Treatment with (MeOH) extract increased the NK activity by 136.27 % in tumor-injected mice, revealing thus an induction of this cell defense system.Fig. 5

Bottom Line: It also promoted lysosomal activity of treated animal macrophages.Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Higher Institute of Medical Technologies of Tunis, Tunis El Manar University, Tunis Rommana, 1068 Tunisia.

ABSTRACT

Background: The purpose of this study was to assess the antitumoral effect of the methanol extract (MeOH) from Nitraria retusa leaves and to investigate its immunomodulatory activity that mediated the prevention of tumor progression in tumor-bearing mice.

Methods: Balb/c mice weighing 18-20 g were subcutaneously implanted with B16-F10 cells then injected intra-peritoneally, 7 days later with (200 mg/kg bw) of MeOH extract, for 21 days. After euthanization on day 21, the tumors were weighed. Lymphocyte proliferation, cytotoxic T lymphocyte (CTL) and NK activity were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring their lysosomal activity and nitric oxide production.

Results: The methanol extract inhibited significantly the growth of the implanted tumor, and increased remarkably splenocyte proliferation as well as NK and CTL activities, in tumor-bearing mice. It also promoted lysosomal activity of treated animal macrophages.

Conclusion: Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.

No MeSH data available.


Related in: MedlinePlus