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Antitumoral potency of methanolic extract from Nitraria retusa leaves via its immunomodulatory effect.

Boubaker J, Chaabane F, Bedoui A, Aloui R, Ahmed BB, Ghedira K, Chekir-Ghedira L - Cancer Cell Int. (2015)

Bottom Line: It also promoted lysosomal activity of treated animal macrophages.Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Higher Institute of Medical Technologies of Tunis, Tunis El Manar University, Tunis Rommana, 1068 Tunisia.

ABSTRACT

Background: The purpose of this study was to assess the antitumoral effect of the methanol extract (MeOH) from Nitraria retusa leaves and to investigate its immunomodulatory activity that mediated the prevention of tumor progression in tumor-bearing mice.

Methods: Balb/c mice weighing 18-20 g were subcutaneously implanted with B16-F10 cells then injected intra-peritoneally, 7 days later with (200 mg/kg bw) of MeOH extract, for 21 days. After euthanization on day 21, the tumors were weighed. Lymphocyte proliferation, cytotoxic T lymphocyte (CTL) and NK activity were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring their lysosomal activity and nitric oxide production.

Results: The methanol extract inhibited significantly the growth of the implanted tumor, and increased remarkably splenocyte proliferation as well as NK and CTL activities, in tumor-bearing mice. It also promoted lysosomal activity of treated animal macrophages.

Conclusion: Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of N. retusa methanol extract on subcutaneous melanoma in Balb/c mice. Mice were sacrificed and then analyzed for representative macroscopic photographs of melanoma tumor weight and volume were determined. a Animal bearing tumor (positive control) (PC), b from animals bearing tumor and treated with MeOH extract at 200 mg/kg b.w
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Fig2: Inhibitory effect of N. retusa methanol extract on subcutaneous melanoma in Balb/c mice. Mice were sacrificed and then analyzed for representative macroscopic photographs of melanoma tumor weight and volume were determined. a Animal bearing tumor (positive control) (PC), b from animals bearing tumor and treated with MeOH extract at 200 mg/kg b.w

Mentions: Our results suggest that (MeOH) extract significantly suppresses growth of tumor cells in the tumor-bearing mice. The average tumor size in (MeOH) extract-treated group was significantly smaller than that in the positive control group (Fig. 2). The inhibition of tumor volume reached 95.19 %. Consistent with this result, the average tumor weight after the administration to animals of the extract, was significantly lower than in positive control (animal with tumor) (PC). The inhibition percentage reached 88.95 % (Fig. 3).Fig. 2


Antitumoral potency of methanolic extract from Nitraria retusa leaves via its immunomodulatory effect.

Boubaker J, Chaabane F, Bedoui A, Aloui R, Ahmed BB, Ghedira K, Chekir-Ghedira L - Cancer Cell Int. (2015)

Inhibitory effect of N. retusa methanol extract on subcutaneous melanoma in Balb/c mice. Mice were sacrificed and then analyzed for representative macroscopic photographs of melanoma tumor weight and volume were determined. a Animal bearing tumor (positive control) (PC), b from animals bearing tumor and treated with MeOH extract at 200 mg/kg b.w
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4549073&req=5

Fig2: Inhibitory effect of N. retusa methanol extract on subcutaneous melanoma in Balb/c mice. Mice were sacrificed and then analyzed for representative macroscopic photographs of melanoma tumor weight and volume were determined. a Animal bearing tumor (positive control) (PC), b from animals bearing tumor and treated with MeOH extract at 200 mg/kg b.w
Mentions: Our results suggest that (MeOH) extract significantly suppresses growth of tumor cells in the tumor-bearing mice. The average tumor size in (MeOH) extract-treated group was significantly smaller than that in the positive control group (Fig. 2). The inhibition of tumor volume reached 95.19 %. Consistent with this result, the average tumor weight after the administration to animals of the extract, was significantly lower than in positive control (animal with tumor) (PC). The inhibition percentage reached 88.95 % (Fig. 3).Fig. 2

Bottom Line: It also promoted lysosomal activity of treated animal macrophages.Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.Abstract available from the publisher.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir, 5000 Tunisia ; Higher Institute of Medical Technologies of Tunis, Tunis El Manar University, Tunis Rommana, 1068 Tunisia.

ABSTRACT

Background: The purpose of this study was to assess the antitumoral effect of the methanol extract (MeOH) from Nitraria retusa leaves and to investigate its immunomodulatory activity that mediated the prevention of tumor progression in tumor-bearing mice.

Methods: Balb/c mice weighing 18-20 g were subcutaneously implanted with B16-F10 cells then injected intra-peritoneally, 7 days later with (200 mg/kg bw) of MeOH extract, for 21 days. After euthanization on day 21, the tumors were weighed. Lymphocyte proliferation, cytotoxic T lymphocyte (CTL) and NK activity were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring their lysosomal activity and nitric oxide production.

Results: The methanol extract inhibited significantly the growth of the implanted tumor, and increased remarkably splenocyte proliferation as well as NK and CTL activities, in tumor-bearing mice. It also promoted lysosomal activity of treated animal macrophages.

Conclusion: Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.

No MeSH data available.


Related in: MedlinePlus