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Genetic Diversity and mRNA Expression of Porcine MHC Class I Chain-Related 2 (SLA-MIC2) Gene and Development of a High-Resolution Typing Method.

Dadi H, Le M, Dinka H, Nguyen D, Choi H, Cho H, Choi M, Kim JH, Park JK, Soundrarajan N, Park C - PLoS ONE (2015)

Bottom Line: The number of alleles of the SLA-MIC2 gene in pigs is somewhat lower compared to the number of alleles of the porcine MHC class I and II genes; however, the level of heterozygosity was similar.Our results indicate the comprehensiveness of using genomic DNA-based typing for the systemic study of the SLA-MIC2 gene.The method developed for this study, as well as the detailed information that was obtained, could serve as fundamental tools for understanding the influence of the SLA-MIC2 gene on porcine immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.

ABSTRACT
The genetic structure and function of MHC class I chain-related (MIC) genes in the pig genome have not been well characterized, and show discordance in available data. Therefore, we have experimentally characterized the exon-intron structure and functional copy expression pattern of the pig MIC gene, SLA-MIC2. We have also studied the genetic diversity of SLA-MIC2 from seven different breeds using a high-resolution genomic sequence-based typing (GSBT) method. Our results showed that the SLA-MIC2 gene has a similar molecular organization as the human and cattle orthologs, and is expressed in only a few tissues including the small intestine, lung, and heart. A total of fifteen SLA-MIC2 alleles were identified from typing 145 animals, ten of which were previously unreported. Our analysis showed that the previously reported and tentatively named SLA-MIC2*05, 07, and 01 alleles occurred most frequently. The observed heterozygosity varied from 0.26 to 0.73 among breeds. The number of alleles of the SLA-MIC2 gene in pigs is somewhat lower compared to the number of alleles of the porcine MHC class I and II genes; however, the level of heterozygosity was similar. Our results indicate the comprehensiveness of using genomic DNA-based typing for the systemic study of the SLA-MIC2 gene. The method developed for this study, as well as the detailed information that was obtained, could serve as fundamental tools for understanding the influence of the SLA-MIC2 gene on porcine immune responses.

No MeSH data available.


Comparison of the mRNA expression levels of SLA-MIC2 in various pig tissues.a) The RT-PCR products (1080 bp) of SLA-MIC2 exons 2 to 6 in different tissues, amplified from RNA isolated from nine-week-old male pigs. b) Standard GAPDH gene expression levels in different tissues, as visualized by the intensity of RT-PCR product staining on an agarose gel. c) The photodensity ratios between the amplified SLA-MIC2 and GAPDH products.
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pone.0135922.g003: Comparison of the mRNA expression levels of SLA-MIC2 in various pig tissues.a) The RT-PCR products (1080 bp) of SLA-MIC2 exons 2 to 6 in different tissues, amplified from RNA isolated from nine-week-old male pigs. b) Standard GAPDH gene expression levels in different tissues, as visualized by the intensity of RT-PCR product staining on an agarose gel. c) The photodensity ratios between the amplified SLA-MIC2 and GAPDH products.

Mentions: We examined the expression of SLA-MIC2 in 15 different pig tissues using semi-quantitative RT-PCR. The comparison of band intensity and semi-quantitative measures of SLA-MIC2 expression (photodensity ratio) showed that SLA-MIC2 was expressed in only the small intestine, lung, and heart, with the most abundant expression in the lung (Fig 3). These findings suggested that the expression patterns of MIC genes among different species are not identical, and that the expression of MIC genes could vary even within a species. The difference in the expression pattern between pigs and humans suggests the presence of possible differences between the MIC-related immune systems of the two species. Further studies are necessary to understand the consequences of these differences.


Genetic Diversity and mRNA Expression of Porcine MHC Class I Chain-Related 2 (SLA-MIC2) Gene and Development of a High-Resolution Typing Method.

Dadi H, Le M, Dinka H, Nguyen D, Choi H, Cho H, Choi M, Kim JH, Park JK, Soundrarajan N, Park C - PLoS ONE (2015)

Comparison of the mRNA expression levels of SLA-MIC2 in various pig tissues.a) The RT-PCR products (1080 bp) of SLA-MIC2 exons 2 to 6 in different tissues, amplified from RNA isolated from nine-week-old male pigs. b) Standard GAPDH gene expression levels in different tissues, as visualized by the intensity of RT-PCR product staining on an agarose gel. c) The photodensity ratios between the amplified SLA-MIC2 and GAPDH products.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549063&req=5

pone.0135922.g003: Comparison of the mRNA expression levels of SLA-MIC2 in various pig tissues.a) The RT-PCR products (1080 bp) of SLA-MIC2 exons 2 to 6 in different tissues, amplified from RNA isolated from nine-week-old male pigs. b) Standard GAPDH gene expression levels in different tissues, as visualized by the intensity of RT-PCR product staining on an agarose gel. c) The photodensity ratios between the amplified SLA-MIC2 and GAPDH products.
Mentions: We examined the expression of SLA-MIC2 in 15 different pig tissues using semi-quantitative RT-PCR. The comparison of band intensity and semi-quantitative measures of SLA-MIC2 expression (photodensity ratio) showed that SLA-MIC2 was expressed in only the small intestine, lung, and heart, with the most abundant expression in the lung (Fig 3). These findings suggested that the expression patterns of MIC genes among different species are not identical, and that the expression of MIC genes could vary even within a species. The difference in the expression pattern between pigs and humans suggests the presence of possible differences between the MIC-related immune systems of the two species. Further studies are necessary to understand the consequences of these differences.

Bottom Line: The number of alleles of the SLA-MIC2 gene in pigs is somewhat lower compared to the number of alleles of the porcine MHC class I and II genes; however, the level of heterozygosity was similar.Our results indicate the comprehensiveness of using genomic DNA-based typing for the systemic study of the SLA-MIC2 gene.The method developed for this study, as well as the detailed information that was obtained, could serve as fundamental tools for understanding the influence of the SLA-MIC2 gene on porcine immune responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, 143-701, Korea.

ABSTRACT
The genetic structure and function of MHC class I chain-related (MIC) genes in the pig genome have not been well characterized, and show discordance in available data. Therefore, we have experimentally characterized the exon-intron structure and functional copy expression pattern of the pig MIC gene, SLA-MIC2. We have also studied the genetic diversity of SLA-MIC2 from seven different breeds using a high-resolution genomic sequence-based typing (GSBT) method. Our results showed that the SLA-MIC2 gene has a similar molecular organization as the human and cattle orthologs, and is expressed in only a few tissues including the small intestine, lung, and heart. A total of fifteen SLA-MIC2 alleles were identified from typing 145 animals, ten of which were previously unreported. Our analysis showed that the previously reported and tentatively named SLA-MIC2*05, 07, and 01 alleles occurred most frequently. The observed heterozygosity varied from 0.26 to 0.73 among breeds. The number of alleles of the SLA-MIC2 gene in pigs is somewhat lower compared to the number of alleles of the porcine MHC class I and II genes; however, the level of heterozygosity was similar. Our results indicate the comprehensiveness of using genomic DNA-based typing for the systemic study of the SLA-MIC2 gene. The method developed for this study, as well as the detailed information that was obtained, could serve as fundamental tools for understanding the influence of the SLA-MIC2 gene on porcine immune responses.

No MeSH data available.