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Proteomic Analysis of Fetal Ovary Reveals That Ovarian Developmental Potential Is Greater in Meishan Pigs than in Yorkshire Pigs.

Xu M, Che L, Wang D, Yang Z, Zhang P, Lin Y, Fang Z, Che L, Li J, Chen D, Wu D, Xu S - PLoS ONE (2015)

Bottom Line: Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation.Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation.These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, 625014, China.

ABSTRACT
Time-dependent expression of functional proteins in fetal ovaries is important to understand the developmental process of the ovary. This study was carried out to enhance our understanding of the developmental process of porcine fetal ovaries and to better address the differences in fetal ovary development of local and foreign pigs. The objective of the present study is to test the expression of key proteins that regulate the growth and development of fetal ovaries in Meishan and Yorkshire porcine breeds by using proteomics technology. Six Meishan and 6 Yorkshire pregnant gilts were used in this experiment. Fetal ovaries were obtained from Yorkshire and Meishan gilts on days 55 and 90 of the gestation period. Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation. Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation. These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity). We also found that alpha-1-antitrypsin, actin, vimentin, and PP2A proteins promote the formation of primordial follicles in the ovaries of Yorkshire pigs on day 55 of gestation while low expression heat shock proteins and high expression alpha-fetoproteins (AFP) may promote Meishan fetal ovarian follicular development on day 90 of gestation. These findings provide a deeper understanding of how reduced expression of heat shock proteins and increased expression of AFP can significantly reduce the risk of reproductive disease in obese Meishan sows. Our study also shows how these proteins can increase the ovulation rate and may be responsible for the low reproductive efficiency reported in other obese breeds. The ovarian developmental potential was found to be greater in Meishan pigs than in Yorkshire pigs.

No MeSH data available.


Related in: MedlinePlus

Classification analysis of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.Ontology analysis of identified Yorkshire and Meishan ovary proteins. The classification of the protein set was performed according to the gene ontology terms: (A) “Molecular function” (19 proteins) and (B) “Biological process” (19 proteins).
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pone.0135514.g003: Classification analysis of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.Ontology analysis of identified Yorkshire and Meishan ovary proteins. The classification of the protein set was performed according to the gene ontology terms: (A) “Molecular function” (19 proteins) and (B) “Biological process” (19 proteins).

Mentions: To understand the biological and molecular effects of proteins on the development of fetal ovaries in Meishan and Yorkshire gilts, differentially-expressed proteins were characterized by GO annotation. The annotated fetal ovarian proteins were identified using the NCBI and porcine databases and were analyzed by the PANTHER system and GO database. Based on molecular functions, proteins were classified as ion binding proteins (41%), RNA binding proteins (11%), enzyme regulators (9%,), unfolded protein-binding proteins (9%), and lipid-binding proteins (6%), among others (Fig 3A) at day 55 of gestation. The identified proteins were involved in the biological processes of transport (15%) and cell death (13%,), followed by signal transduction (6%), biosynthetic response (9%), and stress response (8%), among others (Fig 3B) on day 55 of gestation. It is interesting that alpha-1-antitrypsin that is associated with enzyme regulators and serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform as well as, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform associated with cell death appear to be prominently expressed in Yorkshire gilts on day 55 of gestation.


Proteomic Analysis of Fetal Ovary Reveals That Ovarian Developmental Potential Is Greater in Meishan Pigs than in Yorkshire Pigs.

Xu M, Che L, Wang D, Yang Z, Zhang P, Lin Y, Fang Z, Che L, Li J, Chen D, Wu D, Xu S - PLoS ONE (2015)

Classification analysis of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.Ontology analysis of identified Yorkshire and Meishan ovary proteins. The classification of the protein set was performed according to the gene ontology terms: (A) “Molecular function” (19 proteins) and (B) “Biological process” (19 proteins).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549060&req=5

pone.0135514.g003: Classification analysis of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.Ontology analysis of identified Yorkshire and Meishan ovary proteins. The classification of the protein set was performed according to the gene ontology terms: (A) “Molecular function” (19 proteins) and (B) “Biological process” (19 proteins).
Mentions: To understand the biological and molecular effects of proteins on the development of fetal ovaries in Meishan and Yorkshire gilts, differentially-expressed proteins were characterized by GO annotation. The annotated fetal ovarian proteins were identified using the NCBI and porcine databases and were analyzed by the PANTHER system and GO database. Based on molecular functions, proteins were classified as ion binding proteins (41%), RNA binding proteins (11%), enzyme regulators (9%,), unfolded protein-binding proteins (9%), and lipid-binding proteins (6%), among others (Fig 3A) at day 55 of gestation. The identified proteins were involved in the biological processes of transport (15%) and cell death (13%,), followed by signal transduction (6%), biosynthetic response (9%), and stress response (8%), among others (Fig 3B) on day 55 of gestation. It is interesting that alpha-1-antitrypsin that is associated with enzyme regulators and serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform as well as, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform associated with cell death appear to be prominently expressed in Yorkshire gilts on day 55 of gestation.

Bottom Line: Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation.Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation.These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, 625014, China.

ABSTRACT
Time-dependent expression of functional proteins in fetal ovaries is important to understand the developmental process of the ovary. This study was carried out to enhance our understanding of the developmental process of porcine fetal ovaries and to better address the differences in fetal ovary development of local and foreign pigs. The objective of the present study is to test the expression of key proteins that regulate the growth and development of fetal ovaries in Meishan and Yorkshire porcine breeds by using proteomics technology. Six Meishan and 6 Yorkshire pregnant gilts were used in this experiment. Fetal ovaries were obtained from Yorkshire and Meishan gilts on days 55 and 90 of the gestation period. Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation. Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation. These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity). We also found that alpha-1-antitrypsin, actin, vimentin, and PP2A proteins promote the formation of primordial follicles in the ovaries of Yorkshire pigs on day 55 of gestation while low expression heat shock proteins and high expression alpha-fetoproteins (AFP) may promote Meishan fetal ovarian follicular development on day 90 of gestation. These findings provide a deeper understanding of how reduced expression of heat shock proteins and increased expression of AFP can significantly reduce the risk of reproductive disease in obese Meishan sows. Our study also shows how these proteins can increase the ovulation rate and may be responsible for the low reproductive efficiency reported in other obese breeds. The ovarian developmental potential was found to be greater in Meishan pigs than in Yorkshire pigs.

No MeSH data available.


Related in: MedlinePlus