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Proteomic Analysis of Fetal Ovary Reveals That Ovarian Developmental Potential Is Greater in Meishan Pigs than in Yorkshire Pigs.

Xu M, Che L, Wang D, Yang Z, Zhang P, Lin Y, Fang Z, Che L, Li J, Chen D, Wu D, Xu S - PLoS ONE (2015)

Bottom Line: Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation.Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation.These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, 625014, China.

ABSTRACT
Time-dependent expression of functional proteins in fetal ovaries is important to understand the developmental process of the ovary. This study was carried out to enhance our understanding of the developmental process of porcine fetal ovaries and to better address the differences in fetal ovary development of local and foreign pigs. The objective of the present study is to test the expression of key proteins that regulate the growth and development of fetal ovaries in Meishan and Yorkshire porcine breeds by using proteomics technology. Six Meishan and 6 Yorkshire pregnant gilts were used in this experiment. Fetal ovaries were obtained from Yorkshire and Meishan gilts on days 55 and 90 of the gestation period. Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation. Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation. These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity). We also found that alpha-1-antitrypsin, actin, vimentin, and PP2A proteins promote the formation of primordial follicles in the ovaries of Yorkshire pigs on day 55 of gestation while low expression heat shock proteins and high expression alpha-fetoproteins (AFP) may promote Meishan fetal ovarian follicular development on day 90 of gestation. These findings provide a deeper understanding of how reduced expression of heat shock proteins and increased expression of AFP can significantly reduce the risk of reproductive disease in obese Meishan sows. Our study also shows how these proteins can increase the ovulation rate and may be responsible for the low reproductive efficiency reported in other obese breeds. The ovarian developmental potential was found to be greater in Meishan pigs than in Yorkshire pigs.

No MeSH data available.


Related in: MedlinePlus

Representative DIGE gel image of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.The proteins extracted from the Yorkshire and Meishan fetal ovaries on day 55 of gestation samples were labelled with Cy3 and Cy5, respectively. An internal standard protein sample (a mixture of Yorkshire and Meishan fetal ovaries on day 55 of gestation samples) was labeled with the Cy2 dye. The S2-S11 and S12-S16 spots represent up-regulated proteins, while the others spots represent down-regulated proteins in the in Yorkshire fetal ovaries samples compared with the Meishan fetal ovaries samples. The number in the figure corresponds to the number shown in Table 1.
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pone.0135514.g001: Representative DIGE gel image of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.The proteins extracted from the Yorkshire and Meishan fetal ovaries on day 55 of gestation samples were labelled with Cy3 and Cy5, respectively. An internal standard protein sample (a mixture of Yorkshire and Meishan fetal ovaries on day 55 of gestation samples) was labeled with the Cy2 dye. The S2-S11 and S12-S16 spots represent up-regulated proteins, while the others spots represent down-regulated proteins in the in Yorkshire fetal ovaries samples compared with the Meishan fetal ovaries samples. The number in the figure corresponds to the number shown in Table 1.

Mentions: About 1551 protein spots were detected and 62 individual protein spots were significantly changed (ratioYorkshire/Meishan > 1.5 or ratioMeishan/Yorkshire > 1.5, P < 0.05) on day 55 of gestation in Yorkshire and Meishan fetal ovaries. Twenty seven differentially expressed proteins (ratioYorkshire/Meishan > 1.6 or ratioMeishan/Yorkshire > 1.6, P < 0.05) were selected for MALDI TOF-TOF MS analysis (Fig 1). The proteomic analysis shows that Yorkshire fetal ovaries had 14 upregulated proteins and 13 downregulated proteins compared with Meishan fetal ovaries at day 55 of gestation (Table 1). Yorkshire fetal ovaries express proteins that regulate primordial follicle assembly, such as actin, alpha-1-antitrypsin, vimentin, while Meishan fetal ovaries have more proteins play role on uncleaved oocyte stage, such as serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform and, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform.


Proteomic Analysis of Fetal Ovary Reveals That Ovarian Developmental Potential Is Greater in Meishan Pigs than in Yorkshire Pigs.

Xu M, Che L, Wang D, Yang Z, Zhang P, Lin Y, Fang Z, Che L, Li J, Chen D, Wu D, Xu S - PLoS ONE (2015)

Representative DIGE gel image of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.The proteins extracted from the Yorkshire and Meishan fetal ovaries on day 55 of gestation samples were labelled with Cy3 and Cy5, respectively. An internal standard protein sample (a mixture of Yorkshire and Meishan fetal ovaries on day 55 of gestation samples) was labeled with the Cy2 dye. The S2-S11 and S12-S16 spots represent up-regulated proteins, while the others spots represent down-regulated proteins in the in Yorkshire fetal ovaries samples compared with the Meishan fetal ovaries samples. The number in the figure corresponds to the number shown in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549060&req=5

pone.0135514.g001: Representative DIGE gel image of differentially expressed proteins in Yorkshire and Meishan fetal ovaries at gestation day 55.The proteins extracted from the Yorkshire and Meishan fetal ovaries on day 55 of gestation samples were labelled with Cy3 and Cy5, respectively. An internal standard protein sample (a mixture of Yorkshire and Meishan fetal ovaries on day 55 of gestation samples) was labeled with the Cy2 dye. The S2-S11 and S12-S16 spots represent up-regulated proteins, while the others spots represent down-regulated proteins in the in Yorkshire fetal ovaries samples compared with the Meishan fetal ovaries samples. The number in the figure corresponds to the number shown in Table 1.
Mentions: About 1551 protein spots were detected and 62 individual protein spots were significantly changed (ratioYorkshire/Meishan > 1.5 or ratioMeishan/Yorkshire > 1.5, P < 0.05) on day 55 of gestation in Yorkshire and Meishan fetal ovaries. Twenty seven differentially expressed proteins (ratioYorkshire/Meishan > 1.6 or ratioMeishan/Yorkshire > 1.6, P < 0.05) were selected for MALDI TOF-TOF MS analysis (Fig 1). The proteomic analysis shows that Yorkshire fetal ovaries had 14 upregulated proteins and 13 downregulated proteins compared with Meishan fetal ovaries at day 55 of gestation (Table 1). Yorkshire fetal ovaries express proteins that regulate primordial follicle assembly, such as actin, alpha-1-antitrypsin, vimentin, while Meishan fetal ovaries have more proteins play role on uncleaved oocyte stage, such as serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform and, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform.

Bottom Line: Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation.Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation.These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, 625014, China.

ABSTRACT
Time-dependent expression of functional proteins in fetal ovaries is important to understand the developmental process of the ovary. This study was carried out to enhance our understanding of the developmental process of porcine fetal ovaries and to better address the differences in fetal ovary development of local and foreign pigs. The objective of the present study is to test the expression of key proteins that regulate the growth and development of fetal ovaries in Meishan and Yorkshire porcine breeds by using proteomics technology. Six Meishan and 6 Yorkshire pregnant gilts were used in this experiment. Fetal ovaries were obtained from Yorkshire and Meishan gilts on days 55 and 90 of the gestation period. Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation. Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation. These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity). We also found that alpha-1-antitrypsin, actin, vimentin, and PP2A proteins promote the formation of primordial follicles in the ovaries of Yorkshire pigs on day 55 of gestation while low expression heat shock proteins and high expression alpha-fetoproteins (AFP) may promote Meishan fetal ovarian follicular development on day 90 of gestation. These findings provide a deeper understanding of how reduced expression of heat shock proteins and increased expression of AFP can significantly reduce the risk of reproductive disease in obese Meishan sows. Our study also shows how these proteins can increase the ovulation rate and may be responsible for the low reproductive efficiency reported in other obese breeds. The ovarian developmental potential was found to be greater in Meishan pigs than in Yorkshire pigs.

No MeSH data available.


Related in: MedlinePlus