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Cardiac telocytes are double positive for CD34/PDGFR-α.

Zhou Q, Wei L, Zhong C, Fu S, Bei Y, Huică RI, Wang F, Xiao J - J. Cell. Mol. Med. (2015)

Bottom Line: It has recently been proposed that CD34/PDGFR-α (Platelet-derived growth factor receptor α) is actually a specific marker for TCs including cardiac TCs although the direct evidence is still lacking.We show that cardiac TCs are double positive for CD34/PDGFR-α.Better understanding of the immunocytochemical phenotypes of cardiac TCs might help using cardiac TCs as a novel source in cardiac repair.

View Article: PubMed Central - PubMed

Affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.

No MeSH data available.


Flow cytometry histograms showing CD34, PDGFR-α and PDGFR-β expression for TC-enriched stromal cells from rat heart. Gates with positive events were drawn based on the unstained samples. Filled histograms represent test samples, and unfilled ones show the fluorescence signal for unstained controls.
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fig08: Flow cytometry histograms showing CD34, PDGFR-α and PDGFR-β expression for TC-enriched stromal cells from rat heart. Gates with positive events were drawn based on the unstained samples. Filled histograms represent test samples, and unfilled ones show the fluorescence signal for unstained controls.

Mentions: CD34, PDGFR-α and PDGFR-β expression levels in the whole cell population are shown in Figure7 as percentage positive of the whole cell population. The PDGFR-α+ population varied from 80% to 91% of the total number of cells, with an average value of 87%. The CD34+/PDGFR-α+ population varied from 29% to 41%, while CD34+/PDGFR-β+ represented 28–36% (data not shown). The entire cell population shows a high expression level for PDGFR-α (‘bright’ events) and a lower expression level for CD34 and PDGFR-β (‘dim’ events; Fig.8). CD34/PDGFR-α positive events accounted for 30.25% of the cell population (data not shown).


Cardiac telocytes are double positive for CD34/PDGFR-α.

Zhou Q, Wei L, Zhong C, Fu S, Bei Y, Huică RI, Wang F, Xiao J - J. Cell. Mol. Med. (2015)

Flow cytometry histograms showing CD34, PDGFR-α and PDGFR-β expression for TC-enriched stromal cells from rat heart. Gates with positive events were drawn based on the unstained samples. Filled histograms represent test samples, and unfilled ones show the fluorescence signal for unstained controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549054&req=5

fig08: Flow cytometry histograms showing CD34, PDGFR-α and PDGFR-β expression for TC-enriched stromal cells from rat heart. Gates with positive events were drawn based on the unstained samples. Filled histograms represent test samples, and unfilled ones show the fluorescence signal for unstained controls.
Mentions: CD34, PDGFR-α and PDGFR-β expression levels in the whole cell population are shown in Figure7 as percentage positive of the whole cell population. The PDGFR-α+ population varied from 80% to 91% of the total number of cells, with an average value of 87%. The CD34+/PDGFR-α+ population varied from 29% to 41%, while CD34+/PDGFR-β+ represented 28–36% (data not shown). The entire cell population shows a high expression level for PDGFR-α (‘bright’ events) and a lower expression level for CD34 and PDGFR-β (‘dim’ events; Fig.8). CD34/PDGFR-α positive events accounted for 30.25% of the cell population (data not shown).

Bottom Line: It has recently been proposed that CD34/PDGFR-α (Platelet-derived growth factor receptor α) is actually a specific marker for TCs including cardiac TCs although the direct evidence is still lacking.We show that cardiac TCs are double positive for CD34/PDGFR-α.Better understanding of the immunocytochemical phenotypes of cardiac TCs might help using cardiac TCs as a novel source in cardiac repair.

View Article: PubMed Central - PubMed

Affiliation: Regeneration and Ageing Lab, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.

No MeSH data available.