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The B subunit of Escherichia coli heat-labile toxin alters the development and antigen-presenting capacity of dendritic cells.

Ji J, Griffiths KL, Milburn PJ, Hirst TR, O'Neill HC - J. Cell. Mol. Med. (2015)

Bottom Line: In this respect, the in vivo effect of EtxB differs from that of the highly inflammatory mediator lipopolysaccharide.In terms of the in vivo effect of EtxB on CD4 and CD8 T cell responses in mice, the interaction of EtxB directly with DC was demonstrated following conditional depletion of CD11c(+) DC.In summary, all results are consistent with EtxB displaying adjuvant ability by enhancing the turnover of DC in spleen, leading to newly mature myeloid and DC in spleen, thereby increasing DC capacity to perform as antigen-presenting cells on encounter with T cells.

View Article: PubMed Central - PubMed

Affiliation: Research School of Biology, Australian National University, Canberra, ACT, Australia.

No MeSH data available.


Related in: MedlinePlus

Representation of subsets in spleen. Mature dendritic and myeloid subsets, and dendritic and myeloid precursors, were gated as shown in Figure1. The proportion of each cell type is shown as a percentage of total dendritic and myeloid cells for nine individual mice. Means ± SE are shown by crosshairs. Significantly different pairs at P ≤ 0.05, P ≤ 0.01 and P ≤ 0.001 are identified by *, ** and *** respectively.
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fig02: Representation of subsets in spleen. Mature dendritic and myeloid subsets, and dendritic and myeloid precursors, were gated as shown in Figure1. The proportion of each cell type is shown as a percentage of total dendritic and myeloid cells for nine individual mice. Means ± SE are shown by crosshairs. Significantly different pairs at P ≤ 0.05, P ≤ 0.01 and P ≤ 0.001 are identified by *, ** and *** respectively.

Mentions: In response to 24-hr EtxB treatment, only the DC and myeloid precursor subsets in spleen were found to be significantly reduced (Fig.2). No changes were observed at 72 hrs post-EtxB (data not shown). This effect was EtxB-specific and was lost with EtxB heat inactivation. By comparison, no other DC subsets, including L-DC, pDC and cDC, were affected by treatment of mice with EtxB, although treatment with EtxB gave a significant increase in the number of myeloid cells in spleen. This was lost after heat treatment of EtxB and could reflect an inflammatory response. In resting animals, EtxB appears to accelerate the development of DC and myeloid cells from precursors, rather than induce a dramatic change in the representation of one or more particular subsets.


The B subunit of Escherichia coli heat-labile toxin alters the development and antigen-presenting capacity of dendritic cells.

Ji J, Griffiths KL, Milburn PJ, Hirst TR, O'Neill HC - J. Cell. Mol. Med. (2015)

Representation of subsets in spleen. Mature dendritic and myeloid subsets, and dendritic and myeloid precursors, were gated as shown in Figure1. The proportion of each cell type is shown as a percentage of total dendritic and myeloid cells for nine individual mice. Means ± SE are shown by crosshairs. Significantly different pairs at P ≤ 0.05, P ≤ 0.01 and P ≤ 0.001 are identified by *, ** and *** respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549052&req=5

fig02: Representation of subsets in spleen. Mature dendritic and myeloid subsets, and dendritic and myeloid precursors, were gated as shown in Figure1. The proportion of each cell type is shown as a percentage of total dendritic and myeloid cells for nine individual mice. Means ± SE are shown by crosshairs. Significantly different pairs at P ≤ 0.05, P ≤ 0.01 and P ≤ 0.001 are identified by *, ** and *** respectively.
Mentions: In response to 24-hr EtxB treatment, only the DC and myeloid precursor subsets in spleen were found to be significantly reduced (Fig.2). No changes were observed at 72 hrs post-EtxB (data not shown). This effect was EtxB-specific and was lost with EtxB heat inactivation. By comparison, no other DC subsets, including L-DC, pDC and cDC, were affected by treatment of mice with EtxB, although treatment with EtxB gave a significant increase in the number of myeloid cells in spleen. This was lost after heat treatment of EtxB and could reflect an inflammatory response. In resting animals, EtxB appears to accelerate the development of DC and myeloid cells from precursors, rather than induce a dramatic change in the representation of one or more particular subsets.

Bottom Line: In this respect, the in vivo effect of EtxB differs from that of the highly inflammatory mediator lipopolysaccharide.In terms of the in vivo effect of EtxB on CD4 and CD8 T cell responses in mice, the interaction of EtxB directly with DC was demonstrated following conditional depletion of CD11c(+) DC.In summary, all results are consistent with EtxB displaying adjuvant ability by enhancing the turnover of DC in spleen, leading to newly mature myeloid and DC in spleen, thereby increasing DC capacity to perform as antigen-presenting cells on encounter with T cells.

View Article: PubMed Central - PubMed

Affiliation: Research School of Biology, Australian National University, Canberra, ACT, Australia.

No MeSH data available.


Related in: MedlinePlus