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Notch receptor expression in human brain arteriovenous malformations.

Hill-Felberg S, Wu HH, Toms SA, Dehdashti AR - J. Cell. Mol. Med. (2015)

Bottom Line: We have found that compared to normal brain vascular tissue Notch-3 was dramatically increased in brain arteriovenous malformations.Similarly, Notch 4 labelling was also increased in vascular malformations and was confirmed by western blot analysis.Using both immunohistochemistry on microarrays and western blot analysis, we have found that Notch-1 expression was detectable in control vessels, and discovered a significant decrease of Notch 1 expression in vascular malformations.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Weis Center for Research, Geisinger Health System, Danville, PA, USA.

No MeSH data available.


Related in: MedlinePlus

Western Blot for Notch 4 protein in AVM (A1–4) and control (C1–3) fresh surgical samples. A single band at molecular weight 53 could be detected for Notch 4. Bar graphs represent Total Density after they were normalized with the B-actin internal control antibody. Graphs were made using Graph Pad Prism 5 Software, **P = 0.0030.
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fig05: Western Blot for Notch 4 protein in AVM (A1–4) and control (C1–3) fresh surgical samples. A single band at molecular weight 53 could be detected for Notch 4. Bar graphs represent Total Density after they were normalized with the B-actin internal control antibody. Graphs were made using Graph Pad Prism 5 Software, **P = 0.0030.

Mentions: Overall, Notch 4 had an increase in staining in the BAVM vessels and surrounding tissue (Fig.4B and D) when compared to control epilepsy vessels (Fig.4A and C). We found that the overall labelling intensity did vary among the different BAVM patients analysed. However, age was not a factor with the samples that we analysed. We found a score anywhere from <10% (+/−) to >50% (+++) for Notch 4 labelling, whereas, control arteries generally had the same intensity score of 25% (+) (Table1). Notch 4 labelled the IEL of the blood vessel walls in control vessels (Fig.4A and C). It also appeared to label the endothelial cell layer (Fig.4C). When Notch 4 was combined with elastin staining (pink stain), we confirmed that a portion of Notch 4 was associated with the elastin positive IEL of the artery wall (Fig.4E; see arrow). The majority of the increase in Notch 4 labelling in BAVMs appeared to be due to a diffuse labelling throughout the disrupted vascular tissue (Fig.4B and D). The Notch 4 antibody could be detected on many structures throughout the vessel wall (Fig.4F). The elastin antibody labelling also appeared disrupted (Fig.4F; arrow) similar to the pattern seen with the elastin stain in Figure1F. Western blot analysis for Notch 4 revealed a significant 4.8-fold increase (**P = 0.003) in total Notch 4 protein in the BAVM samples as compared to controls (Fig.5).


Notch receptor expression in human brain arteriovenous malformations.

Hill-Felberg S, Wu HH, Toms SA, Dehdashti AR - J. Cell. Mol. Med. (2015)

Western Blot for Notch 4 protein in AVM (A1–4) and control (C1–3) fresh surgical samples. A single band at molecular weight 53 could be detected for Notch 4. Bar graphs represent Total Density after they were normalized with the B-actin internal control antibody. Graphs were made using Graph Pad Prism 5 Software, **P = 0.0030.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549049&req=5

fig05: Western Blot for Notch 4 protein in AVM (A1–4) and control (C1–3) fresh surgical samples. A single band at molecular weight 53 could be detected for Notch 4. Bar graphs represent Total Density after they were normalized with the B-actin internal control antibody. Graphs were made using Graph Pad Prism 5 Software, **P = 0.0030.
Mentions: Overall, Notch 4 had an increase in staining in the BAVM vessels and surrounding tissue (Fig.4B and D) when compared to control epilepsy vessels (Fig.4A and C). We found that the overall labelling intensity did vary among the different BAVM patients analysed. However, age was not a factor with the samples that we analysed. We found a score anywhere from <10% (+/−) to >50% (+++) for Notch 4 labelling, whereas, control arteries generally had the same intensity score of 25% (+) (Table1). Notch 4 labelled the IEL of the blood vessel walls in control vessels (Fig.4A and C). It also appeared to label the endothelial cell layer (Fig.4C). When Notch 4 was combined with elastin staining (pink stain), we confirmed that a portion of Notch 4 was associated with the elastin positive IEL of the artery wall (Fig.4E; see arrow). The majority of the increase in Notch 4 labelling in BAVMs appeared to be due to a diffuse labelling throughout the disrupted vascular tissue (Fig.4B and D). The Notch 4 antibody could be detected on many structures throughout the vessel wall (Fig.4F). The elastin antibody labelling also appeared disrupted (Fig.4F; arrow) similar to the pattern seen with the elastin stain in Figure1F. Western blot analysis for Notch 4 revealed a significant 4.8-fold increase (**P = 0.003) in total Notch 4 protein in the BAVM samples as compared to controls (Fig.5).

Bottom Line: We have found that compared to normal brain vascular tissue Notch-3 was dramatically increased in brain arteriovenous malformations.Similarly, Notch 4 labelling was also increased in vascular malformations and was confirmed by western blot analysis.Using both immunohistochemistry on microarrays and western blot analysis, we have found that Notch-1 expression was detectable in control vessels, and discovered a significant decrease of Notch 1 expression in vascular malformations.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Weis Center for Research, Geisinger Health System, Danville, PA, USA.

No MeSH data available.


Related in: MedlinePlus