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Notch receptor expression in human brain arteriovenous malformations.

Hill-Felberg S, Wu HH, Toms SA, Dehdashti AR - J. Cell. Mol. Med. (2015)

Bottom Line: We have found that compared to normal brain vascular tissue Notch-3 was dramatically increased in brain arteriovenous malformations.Similarly, Notch 4 labelling was also increased in vascular malformations and was confirmed by western blot analysis.Using both immunohistochemistry on microarrays and western blot analysis, we have found that Notch-1 expression was detectable in control vessels, and discovered a significant decrease of Notch 1 expression in vascular malformations.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Weis Center for Research, Geisinger Health System, Danville, PA, USA.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry for Notch 1 in control (A and C). Arrows indicate Notch 1 present in the interior wall of the blood vessels of the control sections. Arrow head indicates a blood vessel with minimal Notch 1 labelling. BAVM TMA samples (B and D). Arrows indicate vessels with decrease Notch 1 labelling in BAVMs. 200× (A and B), 400× (C and D).
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fig02: Immunohistochemistry for Notch 1 in control (A and C). Arrows indicate Notch 1 present in the interior wall of the blood vessels of the control sections. Arrow head indicates a blood vessel with minimal Notch 1 labelling. BAVM TMA samples (B and D). Arrows indicate vessels with decrease Notch 1 labelling in BAVMs. 200× (A and B), 400× (C and D).

Mentions: The Notch 1 antibody that was used in this study recognized the cleaved intracellular form of Notch 1. When this antibody was used on control and BAVM on our TMA, we found that the overall Notch 1 labelling had decreased in the internal artery vessel walls from a score of approximately 25% (+)in control vessels to a score of approximately <10% (+/−) label in the BAVM vessels (Table1). We identified that Notch 1 (Fig.2) was expressed most intensely on the IEL of the vessel wall in control arteries (Fig.2A and C; see arrows). The labelling appeared to be in the same layer as the elastin antibody labelling from Figure1E. An arrow head indicates a blood vessel where there was minimal Notch 1 labelling (Fig.2C). We identified a few of these throughout the tissue but overwhelmingly the vessels were highly Notch 1 positive in the control samples.


Notch receptor expression in human brain arteriovenous malformations.

Hill-Felberg S, Wu HH, Toms SA, Dehdashti AR - J. Cell. Mol. Med. (2015)

Immunohistochemistry for Notch 1 in control (A and C). Arrows indicate Notch 1 present in the interior wall of the blood vessels of the control sections. Arrow head indicates a blood vessel with minimal Notch 1 labelling. BAVM TMA samples (B and D). Arrows indicate vessels with decrease Notch 1 labelling in BAVMs. 200× (A and B), 400× (C and D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549049&req=5

fig02: Immunohistochemistry for Notch 1 in control (A and C). Arrows indicate Notch 1 present in the interior wall of the blood vessels of the control sections. Arrow head indicates a blood vessel with minimal Notch 1 labelling. BAVM TMA samples (B and D). Arrows indicate vessels with decrease Notch 1 labelling in BAVMs. 200× (A and B), 400× (C and D).
Mentions: The Notch 1 antibody that was used in this study recognized the cleaved intracellular form of Notch 1. When this antibody was used on control and BAVM on our TMA, we found that the overall Notch 1 labelling had decreased in the internal artery vessel walls from a score of approximately 25% (+)in control vessels to a score of approximately <10% (+/−) label in the BAVM vessels (Table1). We identified that Notch 1 (Fig.2) was expressed most intensely on the IEL of the vessel wall in control arteries (Fig.2A and C; see arrows). The labelling appeared to be in the same layer as the elastin antibody labelling from Figure1E. An arrow head indicates a blood vessel where there was minimal Notch 1 labelling (Fig.2C). We identified a few of these throughout the tissue but overwhelmingly the vessels were highly Notch 1 positive in the control samples.

Bottom Line: We have found that compared to normal brain vascular tissue Notch-3 was dramatically increased in brain arteriovenous malformations.Similarly, Notch 4 labelling was also increased in vascular malformations and was confirmed by western blot analysis.Using both immunohistochemistry on microarrays and western blot analysis, we have found that Notch-1 expression was detectable in control vessels, and discovered a significant decrease of Notch 1 expression in vascular malformations.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Weis Center for Research, Geisinger Health System, Danville, PA, USA.

No MeSH data available.


Related in: MedlinePlus