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The CD4(+) AT2R(+) T cell subpopulation improves post-infarction remodelling and restores cardiac function.

Skorska A, von Haehling S, Ludwig M, Lux CA, Gaebel R, Kleiner G, Klopsch C, Dong J, Curato C, Altarche-Xifró W, Slavic S, Unger T, Steinhoff G, Li J, David R - J. Cell. Mol. Med. (2015)

Bottom Line: Flow cytometric analyses showed an increase in the expression of CD4(+) AT2R(+) cells in the rat heart and spleen post-infarction, but a reduction in the peripheral blood.Furthermore, intramyocardial injection of MI-induced splenic CD4(+) AT2R(+) T cells into recipient rats with MI led to reduced infarct size and improved cardiac performance.Our results indicate CD4(+) AT2R(+) cells as a promising population for regenerative therapy, via myocardial transplantation, pharmacological AT2R activation or a combination thereof.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy (RTC)/Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

No MeSH data available.


Related in: MedlinePlus

Adaptive redistribution of the CD4+ AT2R+ T cell population in response to myocardial infarction in rats. Frequency of the CD4+ AT2R+ T cells in CD4+ T cells of blood (sham, n = 7; MI, n = 14), heart (sham, n = 5; MI, n = 12), and spleen (sham, n = 3; MI, n = 7) was evaluated by flow cytometry analysis. Quantitative analysis reveals a significant increase of CD4+ AT2R+ T cells in post-infarct heart and spleen, coincident with a decrease in blood; *P < 0.05, **P < 0.01.
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fig05: Adaptive redistribution of the CD4+ AT2R+ T cell population in response to myocardial infarction in rats. Frequency of the CD4+ AT2R+ T cells in CD4+ T cells of blood (sham, n = 7; MI, n = 14), heart (sham, n = 5; MI, n = 12), and spleen (sham, n = 3; MI, n = 7) was evaluated by flow cytometry analysis. Quantitative analysis reveals a significant increase of CD4+ AT2R+ T cells in post-infarct heart and spleen, coincident with a decrease in blood; *P < 0.05, **P < 0.01.

Mentions: Our recently established methods 18 allowed us to elucidate a potential functional relevance of AT2R for cardiac infiltration with CD4+ T cell subsets. Seven days after MI, we detected CD4+ AT2R+ T cells (Fig. S2A) in infarcted myocardium, circulating blood and spleen. AT2R expression was again verified on the mRNA level. (Fig. S2B). Infiltrating CD4+ AT2R+ T cells in myocardium were mainly detected in the peri-infarct zone (Fig. S2C and Fig.5). In blood, infarcted heart and spleen, 3.8 ± 0.4%, 23.2 ± 2.7% and 22.6 ± 2.6% of CD4+ cells expressed AT2R, respectively (Fig.5). Together, these data reflect an adaptive and selective recruitment of the CD4+ AT2R+ T cell population into the myocardium in response to ischemic insult.


The CD4(+) AT2R(+) T cell subpopulation improves post-infarction remodelling and restores cardiac function.

Skorska A, von Haehling S, Ludwig M, Lux CA, Gaebel R, Kleiner G, Klopsch C, Dong J, Curato C, Altarche-Xifró W, Slavic S, Unger T, Steinhoff G, Li J, David R - J. Cell. Mol. Med. (2015)

Adaptive redistribution of the CD4+ AT2R+ T cell population in response to myocardial infarction in rats. Frequency of the CD4+ AT2R+ T cells in CD4+ T cells of blood (sham, n = 7; MI, n = 14), heart (sham, n = 5; MI, n = 12), and spleen (sham, n = 3; MI, n = 7) was evaluated by flow cytometry analysis. Quantitative analysis reveals a significant increase of CD4+ AT2R+ T cells in post-infarct heart and spleen, coincident with a decrease in blood; *P < 0.05, **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549048&req=5

fig05: Adaptive redistribution of the CD4+ AT2R+ T cell population in response to myocardial infarction in rats. Frequency of the CD4+ AT2R+ T cells in CD4+ T cells of blood (sham, n = 7; MI, n = 14), heart (sham, n = 5; MI, n = 12), and spleen (sham, n = 3; MI, n = 7) was evaluated by flow cytometry analysis. Quantitative analysis reveals a significant increase of CD4+ AT2R+ T cells in post-infarct heart and spleen, coincident with a decrease in blood; *P < 0.05, **P < 0.01.
Mentions: Our recently established methods 18 allowed us to elucidate a potential functional relevance of AT2R for cardiac infiltration with CD4+ T cell subsets. Seven days after MI, we detected CD4+ AT2R+ T cells (Fig. S2A) in infarcted myocardium, circulating blood and spleen. AT2R expression was again verified on the mRNA level. (Fig. S2B). Infiltrating CD4+ AT2R+ T cells in myocardium were mainly detected in the peri-infarct zone (Fig. S2C and Fig.5). In blood, infarcted heart and spleen, 3.8 ± 0.4%, 23.2 ± 2.7% and 22.6 ± 2.6% of CD4+ cells expressed AT2R, respectively (Fig.5). Together, these data reflect an adaptive and selective recruitment of the CD4+ AT2R+ T cell population into the myocardium in response to ischemic insult.

Bottom Line: Flow cytometric analyses showed an increase in the expression of CD4(+) AT2R(+) cells in the rat heart and spleen post-infarction, but a reduction in the peripheral blood.Furthermore, intramyocardial injection of MI-induced splenic CD4(+) AT2R(+) T cells into recipient rats with MI led to reduced infarct size and improved cardiac performance.Our results indicate CD4(+) AT2R(+) cells as a promising population for regenerative therapy, via myocardial transplantation, pharmacological AT2R activation or a combination thereof.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy (RTC)/Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

No MeSH data available.


Related in: MedlinePlus