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Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors.

Abbey JL, Karsunky H, Serwold T, Papathanasiou P, Weissman IL, O'Neill HC - J. Cell. Mol. Med. (2015)

Bottom Line: Cells with the TCR phenotype of Vβ8.2(+) Cβ(-) are found in several lymphoid sites, and among the lineage-negative (Lin(-)) fraction of hematopoietic progenitors in bone marrow (BM).However, B-cell development was detected in spleen.This pattern of restricted in vivo reconstitution disputes Lin(-) Vβ8.2(+) Cβ(-) BM cells as committed T-cell progenitors, but raises the possibility of progenitors with potential for B-cell development.

View Article: PubMed Central - PubMed

Affiliation: Research School of Biology, Australian National University, Canberra, ACT, Australia.

No MeSH data available.


Related in: MedlinePlus

Differentiative potential of Lin−Vβ8+Cβ− bone marrow cells. In the experimental protocol described in Figure3, marker expression was analysed on Ly5.1+ cells present among splenocytes at 4 weeks after intrathymic transfer of Lin−Vβ8+Cβ− BM cells. Splenocytes were stained with antibodies for 5-colour flow cytometric detection of Ly5.1+ cells expressing lineage markers Cβ, CD8, CD4, CD19, Mac1 and Gr-1. Numbers in quadrants reflect % Ly5.1+ cells expressing lineage markers. The staining profile and subset analysis of a single animal are shown. Further animal analyses are summarized in Table1.
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fig04: Differentiative potential of Lin−Vβ8+Cβ− bone marrow cells. In the experimental protocol described in Figure3, marker expression was analysed on Ly5.1+ cells present among splenocytes at 4 weeks after intrathymic transfer of Lin−Vβ8+Cβ− BM cells. Splenocytes were stained with antibodies for 5-colour flow cytometric detection of Ly5.1+ cells expressing lineage markers Cβ, CD8, CD4, CD19, Mac1 and Gr-1. Numbers in quadrants reflect % Ly5.1+ cells expressing lineage markers. The staining profile and subset analysis of a single animal are shown. Further animal analyses are summarized in Table1.

Mentions: Adoptively transferred cells developing in spleen and thymus of the Ly5.2 hosts were first identified as viable (PI−) and then assessed for expression of Ly5.1 and lineage markers. Control Ly5.2 mice given PBS showed no staining for Ly5.1 (Table1). Markers used to detect cell development in thymus were specific for early progenitors (c-Kit), immature T cells (CD44 and CD25), mature αβ T cells (TCR-Cβ, CD3ε, CD4, CD8), TCR-γδ, NK and NK T cells (NK1.1), mature B cells (B220, CD19, I-Ab) and dendritic cells (CD11c, I-Ab). Figure3 shows the staining profile of thymocytes for one representative animal. Cell development in spleen was assessed in terms of the frequency of donor-derived T cells (Cβ, CD4, CD8), B cells (CD19), macrophages (Mac1) and granulocytes (Gr-1). Figure4 shows the staining profile of splenocytes in one representative animal.


Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors.

Abbey JL, Karsunky H, Serwold T, Papathanasiou P, Weissman IL, O'Neill HC - J. Cell. Mol. Med. (2015)

Differentiative potential of Lin−Vβ8+Cβ− bone marrow cells. In the experimental protocol described in Figure3, marker expression was analysed on Ly5.1+ cells present among splenocytes at 4 weeks after intrathymic transfer of Lin−Vβ8+Cβ− BM cells. Splenocytes were stained with antibodies for 5-colour flow cytometric detection of Ly5.1+ cells expressing lineage markers Cβ, CD8, CD4, CD19, Mac1 and Gr-1. Numbers in quadrants reflect % Ly5.1+ cells expressing lineage markers. The staining profile and subset analysis of a single animal are shown. Further animal analyses are summarized in Table1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549046&req=5

fig04: Differentiative potential of Lin−Vβ8+Cβ− bone marrow cells. In the experimental protocol described in Figure3, marker expression was analysed on Ly5.1+ cells present among splenocytes at 4 weeks after intrathymic transfer of Lin−Vβ8+Cβ− BM cells. Splenocytes were stained with antibodies for 5-colour flow cytometric detection of Ly5.1+ cells expressing lineage markers Cβ, CD8, CD4, CD19, Mac1 and Gr-1. Numbers in quadrants reflect % Ly5.1+ cells expressing lineage markers. The staining profile and subset analysis of a single animal are shown. Further animal analyses are summarized in Table1.
Mentions: Adoptively transferred cells developing in spleen and thymus of the Ly5.2 hosts were first identified as viable (PI−) and then assessed for expression of Ly5.1 and lineage markers. Control Ly5.2 mice given PBS showed no staining for Ly5.1 (Table1). Markers used to detect cell development in thymus were specific for early progenitors (c-Kit), immature T cells (CD44 and CD25), mature αβ T cells (TCR-Cβ, CD3ε, CD4, CD8), TCR-γδ, NK and NK T cells (NK1.1), mature B cells (B220, CD19, I-Ab) and dendritic cells (CD11c, I-Ab). Figure3 shows the staining profile of thymocytes for one representative animal. Cell development in spleen was assessed in terms of the frequency of donor-derived T cells (Cβ, CD4, CD8), B cells (CD19), macrophages (Mac1) and granulocytes (Gr-1). Figure4 shows the staining profile of splenocytes in one representative animal.

Bottom Line: Cells with the TCR phenotype of Vβ8.2(+) Cβ(-) are found in several lymphoid sites, and among the lineage-negative (Lin(-)) fraction of hematopoietic progenitors in bone marrow (BM).However, B-cell development was detected in spleen.This pattern of restricted in vivo reconstitution disputes Lin(-) Vβ8.2(+) Cβ(-) BM cells as committed T-cell progenitors, but raises the possibility of progenitors with potential for B-cell development.

View Article: PubMed Central - PubMed

Affiliation: Research School of Biology, Australian National University, Canberra, ACT, Australia.

No MeSH data available.


Related in: MedlinePlus