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Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus

Schematic of the proposed mechanism involved in co-treatment with paclitaxel with letinan-induced cell apoptosis. Combination of paclitaxel and letinan induces ROS over-production, and activates NLRP3 inflammasome and ASK1/p38 MAPK signal, which are contributed to cell apoptosis. ROS: reactive oxygen species; ASK1: apoptosis signal regulating kinase-1; MAPK: mitogen-activated protein kinase; TRX: thioredoxin; TXNIP: thioredoxin-interacting protein.
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fig07: Schematic of the proposed mechanism involved in co-treatment with paclitaxel with letinan-induced cell apoptosis. Combination of paclitaxel and letinan induces ROS over-production, and activates NLRP3 inflammasome and ASK1/p38 MAPK signal, which are contributed to cell apoptosis. ROS: reactive oxygen species; ASK1: apoptosis signal regulating kinase-1; MAPK: mitogen-activated protein kinase; TRX: thioredoxin; TXNIP: thioredoxin-interacting protein.

Mentions: In conclusion, co-treatment with paclitaxel and lentinan plays a synergistic role in enhancing A549 cell apoptosis. Activations of ROS-TXNIP-NLRP3 inflammasome and ASK1/p38 MAPK signal pathways contributed to these synergistic effects (as shown in Fig.7). Our results suggest that combined therapy with paclitaxel and lentinan may have a potential clinical perspective in cancer treatments.


Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Schematic of the proposed mechanism involved in co-treatment with paclitaxel with letinan-induced cell apoptosis. Combination of paclitaxel and letinan induces ROS over-production, and activates NLRP3 inflammasome and ASK1/p38 MAPK signal, which are contributed to cell apoptosis. ROS: reactive oxygen species; ASK1: apoptosis signal regulating kinase-1; MAPK: mitogen-activated protein kinase; TRX: thioredoxin; TXNIP: thioredoxin-interacting protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549045&req=5

fig07: Schematic of the proposed mechanism involved in co-treatment with paclitaxel with letinan-induced cell apoptosis. Combination of paclitaxel and letinan induces ROS over-production, and activates NLRP3 inflammasome and ASK1/p38 MAPK signal, which are contributed to cell apoptosis. ROS: reactive oxygen species; ASK1: apoptosis signal regulating kinase-1; MAPK: mitogen-activated protein kinase; TRX: thioredoxin; TXNIP: thioredoxin-interacting protein.
Mentions: In conclusion, co-treatment with paclitaxel and lentinan plays a synergistic role in enhancing A549 cell apoptosis. Activations of ROS-TXNIP-NLRP3 inflammasome and ASK1/p38 MAPK signal pathways contributed to these synergistic effects (as shown in Fig.7). Our results suggest that combined therapy with paclitaxel and lentinan may have a potential clinical perspective in cancer treatments.

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus