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Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus

Effects of the combination of paclitaxel and letinan on the expressions of p-ASK1 (B), p-p38 MAPK (C) and TXNIP (D). Data represent the mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.
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fig04: Effects of the combination of paclitaxel and letinan on the expressions of p-ASK1 (B), p-p38 MAPK (C) and TXNIP (D). Data represent the mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.

Mentions: Reactive oxygen species acts as a mediator of ASK1, a redox-sensitive mitogen-activated protein kinase kinase kinase, which can increase p38 MAPK protein expression and activity and results in the inhibition of cell proliferation. However, it is unclear whether co-treatment with paclitaxel and letinan modulates ASK1 phosphorylation and p38 MAPK activity in A549 cells. We found that co-treatment with paclitaxel and letinan significantly potentiated the activations of ASK1 and p38 MAPK in A549 cells. Treated with paclitaxel and letinan for 24, 48 and 72 hrs, ASK1 phosphorylation was increased to 0.42 ± 0.07, 0.62 ± 0.1 and 0.78 ± 0.09, respectively. Phosphorylation of p38 MAPK was also time-dependently increased (Fig.4A–C).


Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Effects of the combination of paclitaxel and letinan on the expressions of p-ASK1 (B), p-p38 MAPK (C) and TXNIP (D). Data represent the mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549045&req=5

fig04: Effects of the combination of paclitaxel and letinan on the expressions of p-ASK1 (B), p-p38 MAPK (C) and TXNIP (D). Data represent the mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.
Mentions: Reactive oxygen species acts as a mediator of ASK1, a redox-sensitive mitogen-activated protein kinase kinase kinase, which can increase p38 MAPK protein expression and activity and results in the inhibition of cell proliferation. However, it is unclear whether co-treatment with paclitaxel and letinan modulates ASK1 phosphorylation and p38 MAPK activity in A549 cells. We found that co-treatment with paclitaxel and letinan significantly potentiated the activations of ASK1 and p38 MAPK in A549 cells. Treated with paclitaxel and letinan for 24, 48 and 72 hrs, ASK1 phosphorylation was increased to 0.42 ± 0.07, 0.62 ± 0.1 and 0.78 ± 0.09, respectively. Phosphorylation of p38 MAPK was also time-dependently increased (Fig.4A–C).

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus