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Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus

Effects of the combination of paclitaxel and letinan on ROS production in A549 cells. Data represent the mean ± SEM, n = 4. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.
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fig03: Effects of the combination of paclitaxel and letinan on ROS production in A549 cells. Data represent the mean ± SEM, n = 4. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.

Mentions: Reactive oxygen species is an initiator of the apoptotic response in anti-cancer effects of drugs. So we investigated whether ROS generation was involved in co-treatment of paclitaxel and letinan-induced cell death. As shown in Figure3, DCF fluorescence, indicated the intracellular ROS levels, was increased gradually in A549 cells after combined treatment. Co-treatment with paclitaxel and letinan significantly induced ROS production by 1.7 (48 hrs) - and 2.2 (72 hrs) - fold, respectively.


Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Effects of the combination of paclitaxel and letinan on ROS production in A549 cells. Data represent the mean ± SEM, n = 4. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549045&req=5

fig03: Effects of the combination of paclitaxel and letinan on ROS production in A549 cells. Data represent the mean ± SEM, n = 4. *P < 0.05, **P < 0.01 versus Con group. Con: control group; pac: paclitaxel; let: letinan.
Mentions: Reactive oxygen species is an initiator of the apoptotic response in anti-cancer effects of drugs. So we investigated whether ROS generation was involved in co-treatment of paclitaxel and letinan-induced cell death. As shown in Figure3, DCF fluorescence, indicated the intracellular ROS levels, was increased gradually in A549 cells after combined treatment. Co-treatment with paclitaxel and letinan significantly induced ROS production by 1.7 (48 hrs) - and 2.2 (72 hrs) - fold, respectively.

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus