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Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Bottom Line: Paclitaxel is generally used to treat cancers in clinic as an inhibitor of cell division.We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.In addition, combination of paclitaxel and lentinan could activate apoptosis signal regulating kinase-1 (ASK1)/p38 mitogen-activated protein kinase (MAPK) signal which also contributed to cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus

Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).
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fig01: Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).

Mentions: To determine anti-proliferation effects of the individual compounds, A549 cells were treated with different concentrations of paclitaxel or letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. As shown in Figure1A and B, Paclitaxel or letinan inhibited cell proliferation in a concentration-dependent manner. Paclitaxel or letinan treatment at concentrations ranging from 0.5 to 100 μg/ml decreased proliferation rates from 3.5% to 46.7%, and from 3.3% to 43.3%, respectively. As shown in Figure1C and D, paclitaxel or letinan treatment at the concentration of 5 μg/ml inhibited cell proliferation in a time-dependent manner. Paclitaxel treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 14.3%, 31.4% and 36.4%, respectively. Letinan treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 13.8%, 26.9% and 47.7%, respectively.


Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4549045&req=5

fig01: Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).
Mentions: To determine anti-proliferation effects of the individual compounds, A549 cells were treated with different concentrations of paclitaxel or letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. As shown in Figure1A and B, Paclitaxel or letinan inhibited cell proliferation in a concentration-dependent manner. Paclitaxel or letinan treatment at concentrations ranging from 0.5 to 100 μg/ml decreased proliferation rates from 3.5% to 46.7%, and from 3.3% to 43.3%, respectively. As shown in Figure1C and D, paclitaxel or letinan treatment at the concentration of 5 μg/ml inhibited cell proliferation in a time-dependent manner. Paclitaxel treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 14.3%, 31.4% and 36.4%, respectively. Letinan treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 13.8%, 26.9% and 47.7%, respectively.

Bottom Line: Paclitaxel is generally used to treat cancers in clinic as an inhibitor of cell division.We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.In addition, combination of paclitaxel and lentinan could activate apoptosis signal regulating kinase-1 (ASK1)/p38 mitogen-activated protein kinase (MAPK) signal which also contributed to cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus