Limits...
Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus

Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4549045&req=5

fig01: Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).

Mentions: To determine anti-proliferation effects of the individual compounds, A549 cells were treated with different concentrations of paclitaxel or letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. As shown in Figure1A and B, Paclitaxel or letinan inhibited cell proliferation in a concentration-dependent manner. Paclitaxel or letinan treatment at concentrations ranging from 0.5 to 100 μg/ml decreased proliferation rates from 3.5% to 46.7%, and from 3.3% to 43.3%, respectively. As shown in Figure1C and D, paclitaxel or letinan treatment at the concentration of 5 μg/ml inhibited cell proliferation in a time-dependent manner. Paclitaxel treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 14.3%, 31.4% and 36.4%, respectively. Letinan treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 13.8%, 26.9% and 47.7%, respectively.


Lentinan exerts synergistic apoptotic effects with paclitaxel in A549 cells via activating ROS-TXNIP-NLRP3 inflammasome.

Liu W, Gu J, Qi J, Zeng XN, Ji J, Chen ZZ, Sun XL - J. Cell. Mol. Med. (2015)

Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549045&req=5

fig01: Effects of paclitaxel or letinan alone treatment on A549 cell proliferation. (A) Different concentrations of paclitaxel (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (B) Different concentrations of letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. (C) Paclitaxel alone (5 μg/ml) treated for 72 hrs. (D) Letinan alone (5 μg/ml) treated for 72 hrs. Data represent the mean ± SEM (n = 4).
Mentions: To determine anti-proliferation effects of the individual compounds, A549 cells were treated with different concentrations of paclitaxel or letinan (0.5, 1, 5, 10, 50 and 100 μg/ml) for 24 hrs. As shown in Figure1A and B, Paclitaxel or letinan inhibited cell proliferation in a concentration-dependent manner. Paclitaxel or letinan treatment at concentrations ranging from 0.5 to 100 μg/ml decreased proliferation rates from 3.5% to 46.7%, and from 3.3% to 43.3%, respectively. As shown in Figure1C and D, paclitaxel or letinan treatment at the concentration of 5 μg/ml inhibited cell proliferation in a time-dependent manner. Paclitaxel treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 14.3%, 31.4% and 36.4%, respectively. Letinan treatment alone for 24, 48 and 72 hrs inhibited cell survival rate by 13.8%, 26.9% and 47.7%, respectively.

Bottom Line: We found that the combination of paclitaxel and lentinan resulted in a significantly stronger inhibition on A549 cell proliferation than paclitaxel treatment alone.Furthermore, co-treatment with paclitaxel and lentinan significantly triggered reactive oxygen species (ROS) production, and increased thioredoxin-interacting protein (TXNIP) expression.Taken together, co-treatment with paclitaxel and lentinan exerts synergistic apoptotic effects in A549 cells through inducing ROS production, and activating NLRP3 inflammasome and ASK1/p38 MAPK signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.

No MeSH data available.


Related in: MedlinePlus