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High-density lipoprotein inhibits mechanical stress-induced cardiomyocyte autophagy and cardiac hypertrophy through angiotensin II type 1 receptor-mediated PI3K/Akt pathway.

Lin L, Liu X, Xu J, Weng L, Ren J, Ge J, Zou Y - J. Cell. Mol. Med. (2015)

Bottom Line: Our results indicated that HDL significantly reduced mechanical stretch-induced rise in autophagy as demonstrated by LC3b-II and beclin-1.In addition, mechanical stress up-regulated AT1 receptor expression in both cultured cardiomyocytes and in mouse hearts, whereas HDL significantly suppressed the AT1 receptor.Furthermore, the role of Akt phosphorylation in HDL-mediated action was assessed using MK-2206, a selective inhibitor for Akt phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China.

No MeSH data available.


Related in: MedlinePlus

Effect of HDL on the expression of p-Akt. Western blot analysis for expression of p-Akt; Akt in whole cell lysate used as the loading control; mean ± SEM of 5 mice in all groups. **P < 0.01 versus control.
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fig06: Effect of HDL on the expression of p-Akt. Western blot analysis for expression of p-Akt; Akt in whole cell lysate used as the loading control; mean ± SEM of 5 mice in all groups. **P < 0.01 versus control.

Mentions: As previously reported, PI3K/Akt pathway played a critical role in autophagy 25. To determine if HDL exerts its action through PI3K/Akt pathway, we examined the phosphorylation levels of Akt in HDL-treated cardiomyocytes. The phosphorylation was significantly increased by HDL (Fig.6). Interestingly, MK-2206, a selective inhibitor for Akt phosphorylation, prevented HDL-elicited response against mechanical stress-induced autophagy both in vitro (Fig.2A and B) and in vivo (Fig.4A and B).


High-density lipoprotein inhibits mechanical stress-induced cardiomyocyte autophagy and cardiac hypertrophy through angiotensin II type 1 receptor-mediated PI3K/Akt pathway.

Lin L, Liu X, Xu J, Weng L, Ren J, Ge J, Zou Y - J. Cell. Mol. Med. (2015)

Effect of HDL on the expression of p-Akt. Western blot analysis for expression of p-Akt; Akt in whole cell lysate used as the loading control; mean ± SEM of 5 mice in all groups. **P < 0.01 versus control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549043&req=5

fig06: Effect of HDL on the expression of p-Akt. Western blot analysis for expression of p-Akt; Akt in whole cell lysate used as the loading control; mean ± SEM of 5 mice in all groups. **P < 0.01 versus control.
Mentions: As previously reported, PI3K/Akt pathway played a critical role in autophagy 25. To determine if HDL exerts its action through PI3K/Akt pathway, we examined the phosphorylation levels of Akt in HDL-treated cardiomyocytes. The phosphorylation was significantly increased by HDL (Fig.6). Interestingly, MK-2206, a selective inhibitor for Akt phosphorylation, prevented HDL-elicited response against mechanical stress-induced autophagy both in vitro (Fig.2A and B) and in vivo (Fig.4A and B).

Bottom Line: Our results indicated that HDL significantly reduced mechanical stretch-induced rise in autophagy as demonstrated by LC3b-II and beclin-1.In addition, mechanical stress up-regulated AT1 receptor expression in both cultured cardiomyocytes and in mouse hearts, whereas HDL significantly suppressed the AT1 receptor.Furthermore, the role of Akt phosphorylation in HDL-mediated action was assessed using MK-2206, a selective inhibitor for Akt phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China.

No MeSH data available.


Related in: MedlinePlus