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High-density lipoprotein inhibits mechanical stress-induced cardiomyocyte autophagy and cardiac hypertrophy through angiotensin II type 1 receptor-mediated PI3K/Akt pathway.

Lin L, Liu X, Xu J, Weng L, Ren J, Ge J, Zou Y - J. Cell. Mol. Med. (2015)

Bottom Line: Our results indicated that HDL significantly reduced mechanical stretch-induced rise in autophagy as demonstrated by LC3b-II and beclin-1.In addition, mechanical stress up-regulated AT1 receptor expression in both cultured cardiomyocytes and in mouse hearts, whereas HDL significantly suppressed the AT1 receptor.Furthermore, the role of Akt phosphorylation in HDL-mediated action was assessed using MK-2206, a selective inhibitor for Akt phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China.

No MeSH data available.


Related in: MedlinePlus

Effect of HDL on the expression of AT1 receptor. Membrane proteins were extracted from cardiomyocytes and LV of mice. Western blot analyses were employed to determine the expression of AT1 receptor using an anti-AT1 receptor antibody. β-actin was used as the loading control. Representative photograms from 3 independent experiments with cardiomyocytes or from 5 mouse hearts are shown.
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fig05: Effect of HDL on the expression of AT1 receptor. Membrane proteins were extracted from cardiomyocytes and LV of mice. Western blot analyses were employed to determine the expression of AT1 receptor using an anti-AT1 receptor antibody. β-actin was used as the loading control. Representative photograms from 3 independent experiments with cardiomyocytes or from 5 mouse hearts are shown.

Mentions: The AT1 receptor is believed to play an essential role in the development of cardiac hypertrophy and autophagy 24. Here, we tested whether HDL-elicited inhibitory effect on mechanical stress-induced cardiac hypertrophy and autophagy was due to inhibition of AT1 receptor. Our data revealed that mechanical stress triggered up-regulation of AT1 receptor in both cultured cardiomyocytes and hearts, the effects of which were reconciled by HDL (Fig.5). These findings suggest that downregulation of the AT1 receptor may contribute to HDL-elicited beneficial effect against cardiac hypertrophy and autophagy.


High-density lipoprotein inhibits mechanical stress-induced cardiomyocyte autophagy and cardiac hypertrophy through angiotensin II type 1 receptor-mediated PI3K/Akt pathway.

Lin L, Liu X, Xu J, Weng L, Ren J, Ge J, Zou Y - J. Cell. Mol. Med. (2015)

Effect of HDL on the expression of AT1 receptor. Membrane proteins were extracted from cardiomyocytes and LV of mice. Western blot analyses were employed to determine the expression of AT1 receptor using an anti-AT1 receptor antibody. β-actin was used as the loading control. Representative photograms from 3 independent experiments with cardiomyocytes or from 5 mouse hearts are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549043&req=5

fig05: Effect of HDL on the expression of AT1 receptor. Membrane proteins were extracted from cardiomyocytes and LV of mice. Western blot analyses were employed to determine the expression of AT1 receptor using an anti-AT1 receptor antibody. β-actin was used as the loading control. Representative photograms from 3 independent experiments with cardiomyocytes or from 5 mouse hearts are shown.
Mentions: The AT1 receptor is believed to play an essential role in the development of cardiac hypertrophy and autophagy 24. Here, we tested whether HDL-elicited inhibitory effect on mechanical stress-induced cardiac hypertrophy and autophagy was due to inhibition of AT1 receptor. Our data revealed that mechanical stress triggered up-regulation of AT1 receptor in both cultured cardiomyocytes and hearts, the effects of which were reconciled by HDL (Fig.5). These findings suggest that downregulation of the AT1 receptor may contribute to HDL-elicited beneficial effect against cardiac hypertrophy and autophagy.

Bottom Line: Our results indicated that HDL significantly reduced mechanical stretch-induced rise in autophagy as demonstrated by LC3b-II and beclin-1.In addition, mechanical stress up-regulated AT1 receptor expression in both cultured cardiomyocytes and in mouse hearts, whereas HDL significantly suppressed the AT1 receptor.Furthermore, the role of Akt phosphorylation in HDL-mediated action was assessed using MK-2206, a selective inhibitor for Akt phosphorylation.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China.

No MeSH data available.


Related in: MedlinePlus