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Acacetin inhibits expression of matrix metalloproteinases via a MAPK-dependent mechanism in fibroblast-like synoviocytes.

Chen WP, Yang ZG, Hu PF, Bao JP, Wu LD - J. Cell. Mol. Med. (2015)

Bottom Line: We found that acacetin inhibits p38 and JNK phosphorylation and reduces MMP-1, MMP-3 and MMP-13 expression in interleukin-1β-induced FLSs.Our results suggest that acacetin has antiarthritic effects in FLSs.Thus, acacetin should be further studied for the treatment of arthritis.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

No MeSH data available.


Related in: MedlinePlus

Effect of acacetin on cell viability. Cells were seeded in 96-well plates at 6 × 103/well and cultured with different concentrations of acacetin for 24 hrs, followed by MTT assay analysis. Data are expressed as means ± SD. *P < 0.05 compared with cells treated with culture medium only.
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fig01: Effect of acacetin on cell viability. Cells were seeded in 96-well plates at 6 × 103/well and cultured with different concentrations of acacetin for 24 hrs, followed by MTT assay analysis. Data are expressed as means ± SD. *P < 0.05 compared with cells treated with culture medium only.

Mentions: MTT assays showed that acacetin at the concentrations of 1, 5 and 10 μM showed no cytotoxicity. However, higher concentrations showed significant cytotoxicity (Fig.1). Thus, 10 μM acacetin was used in subsequent experiments.


Acacetin inhibits expression of matrix metalloproteinases via a MAPK-dependent mechanism in fibroblast-like synoviocytes.

Chen WP, Yang ZG, Hu PF, Bao JP, Wu LD - J. Cell. Mol. Med. (2015)

Effect of acacetin on cell viability. Cells were seeded in 96-well plates at 6 × 103/well and cultured with different concentrations of acacetin for 24 hrs, followed by MTT assay analysis. Data are expressed as means ± SD. *P < 0.05 compared with cells treated with culture medium only.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549041&req=5

fig01: Effect of acacetin on cell viability. Cells were seeded in 96-well plates at 6 × 103/well and cultured with different concentrations of acacetin for 24 hrs, followed by MTT assay analysis. Data are expressed as means ± SD. *P < 0.05 compared with cells treated with culture medium only.
Mentions: MTT assays showed that acacetin at the concentrations of 1, 5 and 10 μM showed no cytotoxicity. However, higher concentrations showed significant cytotoxicity (Fig.1). Thus, 10 μM acacetin was used in subsequent experiments.

Bottom Line: We found that acacetin inhibits p38 and JNK phosphorylation and reduces MMP-1, MMP-3 and MMP-13 expression in interleukin-1β-induced FLSs.Our results suggest that acacetin has antiarthritic effects in FLSs.Thus, acacetin should be further studied for the treatment of arthritis.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

No MeSH data available.


Related in: MedlinePlus