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Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells.

da Silva LB, Poulsen JN, Arendt-Nielsen L, Gazerani P - J. Cell. Mol. Med. (2015)

Bottom Line: Ionomycin significantly increased glutamate release from cultured SGCs 30 min. following the treatment (P < 0.001).BoNTA (100 pM) significantly decreased glutamate release (P < 0.01).In addition, interaction of BoNTA with non-neuronal cells at the level of TG suggests a potential analgesic mechanism of action of BoNTA.

View Article: PubMed Central - PubMed

Affiliation: Center for Sensory - Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.

No MeSH data available.


Related in: MedlinePlus

Immunocytochemistry of 7-day-old primary cultures of satellite glial cells from rat trigeminal ganglia. Image (A), shows the nuclei of the satellite glial cell stained with the nuclear stain Hoechst. The cultures are double labelled with antibodies against glutamine synthetase (Red: Alexa fluor® 555) (B) and SNAP-23 (Green: Alexa fluor® 488) (C). The merged image (D) is an overlay of all channels and this image shows the co-localization of glutamine synthetase and SNAP-23 in cultured rat trigeminal satellite glial cells. Virtually all SGCs seem SNAP-23 positive.
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fig04: Immunocytochemistry of 7-day-old primary cultures of satellite glial cells from rat trigeminal ganglia. Image (A), shows the nuclei of the satellite glial cell stained with the nuclear stain Hoechst. The cultures are double labelled with antibodies against glutamine synthetase (Red: Alexa fluor® 555) (B) and SNAP-23 (Green: Alexa fluor® 488) (C). The merged image (D) is an overlay of all channels and this image shows the co-localization of glutamine synthetase and SNAP-23 in cultured rat trigeminal satellite glial cells. Virtually all SGCs seem SNAP-23 positive.

Mentions: Fluorescence microscopy was used to identify the expression of SNAP-25 and SNAP-23 in SGCs. The SGCs were characterized by double labelling with GS, a specific marker for SGCs in the peripheral ganglia. Figures1 and 2 show, respectively, the immunoreactivity of SNAP-25 and SNAP-23 and how they were distributed. Double labelling between SNAP-25 or SNAP-23 and GS was used to identify positive SGCs for SNAP in cryostat tissue sections of the TG. SNAP-25 and SNAP-23 were also present and observed in cultured cells as presented in Figures3 and 4, respectively.


Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells.

da Silva LB, Poulsen JN, Arendt-Nielsen L, Gazerani P - J. Cell. Mol. Med. (2015)

Immunocytochemistry of 7-day-old primary cultures of satellite glial cells from rat trigeminal ganglia. Image (A), shows the nuclei of the satellite glial cell stained with the nuclear stain Hoechst. The cultures are double labelled with antibodies against glutamine synthetase (Red: Alexa fluor® 555) (B) and SNAP-23 (Green: Alexa fluor® 488) (C). The merged image (D) is an overlay of all channels and this image shows the co-localization of glutamine synthetase and SNAP-23 in cultured rat trigeminal satellite glial cells. Virtually all SGCs seem SNAP-23 positive.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549040&req=5

fig04: Immunocytochemistry of 7-day-old primary cultures of satellite glial cells from rat trigeminal ganglia. Image (A), shows the nuclei of the satellite glial cell stained with the nuclear stain Hoechst. The cultures are double labelled with antibodies against glutamine synthetase (Red: Alexa fluor® 555) (B) and SNAP-23 (Green: Alexa fluor® 488) (C). The merged image (D) is an overlay of all channels and this image shows the co-localization of glutamine synthetase and SNAP-23 in cultured rat trigeminal satellite glial cells. Virtually all SGCs seem SNAP-23 positive.
Mentions: Fluorescence microscopy was used to identify the expression of SNAP-25 and SNAP-23 in SGCs. The SGCs were characterized by double labelling with GS, a specific marker for SGCs in the peripheral ganglia. Figures1 and 2 show, respectively, the immunoreactivity of SNAP-25 and SNAP-23 and how they were distributed. Double labelling between SNAP-25 or SNAP-23 and GS was used to identify positive SGCs for SNAP in cryostat tissue sections of the TG. SNAP-25 and SNAP-23 were also present and observed in cultured cells as presented in Figures3 and 4, respectively.

Bottom Line: Ionomycin significantly increased glutamate release from cultured SGCs 30 min. following the treatment (P < 0.001).BoNTA (100 pM) significantly decreased glutamate release (P < 0.01).In addition, interaction of BoNTA with non-neuronal cells at the level of TG suggests a potential analgesic mechanism of action of BoNTA.

View Article: PubMed Central - PubMed

Affiliation: Center for Sensory - Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.

No MeSH data available.


Related in: MedlinePlus