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Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells.

da Silva LB, Poulsen JN, Arendt-Nielsen L, Gazerani P - J. Cell. Mol. Med. (2015)

Bottom Line: Ionomycin significantly increased glutamate release from cultured SGCs 30 min. following the treatment (P < 0.001).BoNTA (100 pM) significantly decreased glutamate release (P < 0.01).In addition, interaction of BoNTA with non-neuronal cells at the level of TG suggests a potential analgesic mechanism of action of BoNTA.

View Article: PubMed Central - PubMed

Affiliation: Center for Sensory - Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.

No MeSH data available.


Related in: MedlinePlus

Localization of SNAP-23 in trigeminal satellite glial cells in rat. The blue colour on image (A) represents the nuclear stain Hoechst and stains the nuclei of each cell. SNAP23 positive satellite glial cells were identified by double labelling with antibodies against glutamine synthetase (Red: Alexa fluor® 555), a specific Satellite glial cell marker in ganglia (B) and SNAP-23 (Green: Alexa fluor® 488) (C). On the merged image (D) overlapping of the two colours indicates SNAP23 positive SGCs. The enhanced area on image (D) shows an example of such co-localization of satellite glial cells enveloping a neuron.
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fig02: Localization of SNAP-23 in trigeminal satellite glial cells in rat. The blue colour on image (A) represents the nuclear stain Hoechst and stains the nuclei of each cell. SNAP23 positive satellite glial cells were identified by double labelling with antibodies against glutamine synthetase (Red: Alexa fluor® 555), a specific Satellite glial cell marker in ganglia (B) and SNAP-23 (Green: Alexa fluor® 488) (C). On the merged image (D) overlapping of the two colours indicates SNAP23 positive SGCs. The enhanced area on image (D) shows an example of such co-localization of satellite glial cells enveloping a neuron.

Mentions: Fluorescence microscopy was used to identify the expression of SNAP-25 and SNAP-23 in SGCs. The SGCs were characterized by double labelling with GS, a specific marker for SGCs in the peripheral ganglia. Figures1 and 2 show, respectively, the immunoreactivity of SNAP-25 and SNAP-23 and how they were distributed. Double labelling between SNAP-25 or SNAP-23 and GS was used to identify positive SGCs for SNAP in cryostat tissue sections of the TG. SNAP-25 and SNAP-23 were also present and observed in cultured cells as presented in Figures3 and 4, respectively.


Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells.

da Silva LB, Poulsen JN, Arendt-Nielsen L, Gazerani P - J. Cell. Mol. Med. (2015)

Localization of SNAP-23 in trigeminal satellite glial cells in rat. The blue colour on image (A) represents the nuclear stain Hoechst and stains the nuclei of each cell. SNAP23 positive satellite glial cells were identified by double labelling with antibodies against glutamine synthetase (Red: Alexa fluor® 555), a specific Satellite glial cell marker in ganglia (B) and SNAP-23 (Green: Alexa fluor® 488) (C). On the merged image (D) overlapping of the two colours indicates SNAP23 positive SGCs. The enhanced area on image (D) shows an example of such co-localization of satellite glial cells enveloping a neuron.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549040&req=5

fig02: Localization of SNAP-23 in trigeminal satellite glial cells in rat. The blue colour on image (A) represents the nuclear stain Hoechst and stains the nuclei of each cell. SNAP23 positive satellite glial cells were identified by double labelling with antibodies against glutamine synthetase (Red: Alexa fluor® 555), a specific Satellite glial cell marker in ganglia (B) and SNAP-23 (Green: Alexa fluor® 488) (C). On the merged image (D) overlapping of the two colours indicates SNAP23 positive SGCs. The enhanced area on image (D) shows an example of such co-localization of satellite glial cells enveloping a neuron.
Mentions: Fluorescence microscopy was used to identify the expression of SNAP-25 and SNAP-23 in SGCs. The SGCs were characterized by double labelling with GS, a specific marker for SGCs in the peripheral ganglia. Figures1 and 2 show, respectively, the immunoreactivity of SNAP-25 and SNAP-23 and how they were distributed. Double labelling between SNAP-25 or SNAP-23 and GS was used to identify positive SGCs for SNAP in cryostat tissue sections of the TG. SNAP-25 and SNAP-23 were also present and observed in cultured cells as presented in Figures3 and 4, respectively.

Bottom Line: Ionomycin significantly increased glutamate release from cultured SGCs 30 min. following the treatment (P < 0.001).BoNTA (100 pM) significantly decreased glutamate release (P < 0.01).In addition, interaction of BoNTA with non-neuronal cells at the level of TG suggests a potential analgesic mechanism of action of BoNTA.

View Article: PubMed Central - PubMed

Affiliation: Center for Sensory - Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.

No MeSH data available.


Related in: MedlinePlus