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Beneficial effects of intramyocardial mesenchymal stem cells and VEGF165 plasmid injection in rats with furazolidone induced dilated cardiomyopathy.

Yu Q, Fang W, Zhu N, Zheng X, Na R, Liu B, Meng L, Li Z, Li Q, Li X - J. Cell. Mol. Med. (2015)

Bottom Line: Cardiac function was improved in MSCs, GENE and MSCs+GENE groups.Collagen volume fraction was significantly reduced and myocardial TGF-β1 mRNA expression significantly down-regulated in both GENE and MSCs+GENE groups, collagen type I/III ratio reduction was more significant in MSCs+GENE group than in MSCs or GENE group.Myocardial MSCs and hVEGF165 plasmid injection improves cardiac function possibly through down-regulating myocardial TGF-β1 expression and reducing the type I/III collagen ratio in this DCM rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.

No MeSH data available.


Related in: MedlinePlus

Representative RT-PCR electrophoresis gel (1: Maker; 2: PBS group; 3: MSCs+ GENE group; 4: MSCs group; 5: GENE group). Note that myocardial mRNA expression of TGF-β1 and collagen I were decreased in GENE and MSCs+GENE groups compared to PBS group, mRNA expression of collagen III was increased in MSCs group while decreased in GENE group. Collagen I/III ratio was reduced in MSCs and GENE groups and in MSCs+ GENE group.
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fig06: Representative RT-PCR electrophoresis gel (1: Maker; 2: PBS group; 3: MSCs+ GENE group; 4: MSCs group; 5: GENE group). Note that myocardial mRNA expression of TGF-β1 and collagen I were decreased in GENE and MSCs+GENE groups compared to PBS group, mRNA expression of collagen III was increased in MSCs group while decreased in GENE group. Collagen I/III ratio was reduced in MSCs and GENE groups and in MSCs+ GENE group.

Mentions: The results of RT-PCR (Fig.6) showed that myocardial mRNA expressions of TGF-β1 and collagen I were down-regulated in GENE and MSCs+GENE group compared to PBS group, mRNA expression of collagen III was up-regulated in MSCs group while down-regulated in GENE group. Collagen I/III ratio was significantly reduced in MSCs and GENE group and further reduced in MSCs+GENE group. Myocardial MMP-9 expression also tended to be higher in MSCs and GENE groups compared to PBS group (Fig.7).


Beneficial effects of intramyocardial mesenchymal stem cells and VEGF165 plasmid injection in rats with furazolidone induced dilated cardiomyopathy.

Yu Q, Fang W, Zhu N, Zheng X, Na R, Liu B, Meng L, Li Z, Li Q, Li X - J. Cell. Mol. Med. (2015)

Representative RT-PCR electrophoresis gel (1: Maker; 2: PBS group; 3: MSCs+ GENE group; 4: MSCs group; 5: GENE group). Note that myocardial mRNA expression of TGF-β1 and collagen I were decreased in GENE and MSCs+GENE groups compared to PBS group, mRNA expression of collagen III was increased in MSCs group while decreased in GENE group. Collagen I/III ratio was reduced in MSCs and GENE groups and in MSCs+ GENE group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549037&req=5

fig06: Representative RT-PCR electrophoresis gel (1: Maker; 2: PBS group; 3: MSCs+ GENE group; 4: MSCs group; 5: GENE group). Note that myocardial mRNA expression of TGF-β1 and collagen I were decreased in GENE and MSCs+GENE groups compared to PBS group, mRNA expression of collagen III was increased in MSCs group while decreased in GENE group. Collagen I/III ratio was reduced in MSCs and GENE groups and in MSCs+ GENE group.
Mentions: The results of RT-PCR (Fig.6) showed that myocardial mRNA expressions of TGF-β1 and collagen I were down-regulated in GENE and MSCs+GENE group compared to PBS group, mRNA expression of collagen III was up-regulated in MSCs group while down-regulated in GENE group. Collagen I/III ratio was significantly reduced in MSCs and GENE group and further reduced in MSCs+GENE group. Myocardial MMP-9 expression also tended to be higher in MSCs and GENE groups compared to PBS group (Fig.7).

Bottom Line: Cardiac function was improved in MSCs, GENE and MSCs+GENE groups.Collagen volume fraction was significantly reduced and myocardial TGF-β1 mRNA expression significantly down-regulated in both GENE and MSCs+GENE groups, collagen type I/III ratio reduction was more significant in MSCs+GENE group than in MSCs or GENE group.Myocardial MSCs and hVEGF165 plasmid injection improves cardiac function possibly through down-regulating myocardial TGF-β1 expression and reducing the type I/III collagen ratio in this DCM rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.

No MeSH data available.


Related in: MedlinePlus