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Beneficial effects of intramyocardial mesenchymal stem cells and VEGF165 plasmid injection in rats with furazolidone induced dilated cardiomyopathy.

Yu Q, Fang W, Zhu N, Zheng X, Na R, Liu B, Meng L, Li Z, Li Q, Li X - J. Cell. Mol. Med. (2015)

Bottom Line: Cardiac function was improved in MSCs, GENE and MSCs+GENE groups.Collagen volume fraction was significantly reduced and myocardial TGF-β1 mRNA expression significantly down-regulated in both GENE and MSCs+GENE groups, collagen type I/III ratio reduction was more significant in MSCs+GENE group than in MSCs or GENE group.Myocardial MSCs and hVEGF165 plasmid injection improves cardiac function possibly through down-regulating myocardial TGF-β1 expression and reducing the type I/III collagen ratio in this DCM rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.

No MeSH data available.


Related in: MedlinePlus

Representative results of identification of MSCs by fluorescence-activated cell sorting (FACS). (A) (negative control); (B) the positive rate of MSCs cells expressing CD34 was 0.48%; (C) expressing CD44 was 79.45%; (D) expressing CD90 was 67.41%.
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fig01: Representative results of identification of MSCs by fluorescence-activated cell sorting (FACS). (A) (negative control); (B) the positive rate of MSCs cells expressing CD34 was 0.48%; (C) expressing CD44 was 79.45%; (D) expressing CD90 was 67.41%.

Mentions: Cultured MSCs were analysed and identified by fluorescence-activated cell sorting. Cells were incubated with fluorescein isothiocyanate–conjugated mouse monoclonal antibodies against rat CD44, CD34 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), CD90 BD (Becton, Dickinson and Company., Franklin Lakes, NJ, U.S.A). Mesenchymal stem cells are known to express CD90, CD44 but not CD34. Our results showed that the positive rate of MSCs cells expressing CD34+ was 0.48% and expressing CD44+ was 79.45%, expressing CD90+ was 67.41% (Fig.1). Above expression patterns are consistent with MSCs 22.


Beneficial effects of intramyocardial mesenchymal stem cells and VEGF165 plasmid injection in rats with furazolidone induced dilated cardiomyopathy.

Yu Q, Fang W, Zhu N, Zheng X, Na R, Liu B, Meng L, Li Z, Li Q, Li X - J. Cell. Mol. Med. (2015)

Representative results of identification of MSCs by fluorescence-activated cell sorting (FACS). (A) (negative control); (B) the positive rate of MSCs cells expressing CD34 was 0.48%; (C) expressing CD44 was 79.45%; (D) expressing CD90 was 67.41%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549037&req=5

fig01: Representative results of identification of MSCs by fluorescence-activated cell sorting (FACS). (A) (negative control); (B) the positive rate of MSCs cells expressing CD34 was 0.48%; (C) expressing CD44 was 79.45%; (D) expressing CD90 was 67.41%.
Mentions: Cultured MSCs were analysed and identified by fluorescence-activated cell sorting. Cells were incubated with fluorescein isothiocyanate–conjugated mouse monoclonal antibodies against rat CD44, CD34 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), CD90 BD (Becton, Dickinson and Company., Franklin Lakes, NJ, U.S.A). Mesenchymal stem cells are known to express CD90, CD44 but not CD34. Our results showed that the positive rate of MSCs cells expressing CD34+ was 0.48% and expressing CD44+ was 79.45%, expressing CD90+ was 67.41% (Fig.1). Above expression patterns are consistent with MSCs 22.

Bottom Line: Cardiac function was improved in MSCs, GENE and MSCs+GENE groups.Collagen volume fraction was significantly reduced and myocardial TGF-β1 mRNA expression significantly down-regulated in both GENE and MSCs+GENE groups, collagen type I/III ratio reduction was more significant in MSCs+GENE group than in MSCs or GENE group.Myocardial MSCs and hVEGF165 plasmid injection improves cardiac function possibly through down-regulating myocardial TGF-β1 expression and reducing the type I/III collagen ratio in this DCM rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian, China.

No MeSH data available.


Related in: MedlinePlus