Limits...
High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss.

Zeng H, Vaka VR, He X, Booz GW, Chen JX - J. Cell. Mol. Med. (2015)

Bottom Line: HFD resulted in a significant reduction in SIRT3 expression in the heart.SIRT3 KO mice fed HFD showed greater ROS production and a further reduction in cardiac function compared to SIRT3 KO mice on ND.However, HFD did not further reduce capillary density in SIRT3 KO hearts, implicating SIRT3 loss in HFD-induced capillary rarefaction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, School of Medicine, Jackson, MS, USA.

No MeSH data available.


Related in: MedlinePlus

HFD decreases SIRT3 expression in the heart. (A) WT mice were fed ND or HFD for 16 weeks. Hearts were extracted and Western blot analysis was performed on ventricular lysates. The blot was probed for SIRT3 expression. The blot was stripped and reprobed for GAPDH as a loading control. Bar graph shows quantification of expression. Values are mean ± SEM, n = 6 mice per group. **P < 0.01. (B) Confirmation that KO mice do not express SIRT3. Protein extracts from the ventricles and isolated mitochondria of WT and SIRT3 KO mouse hearts were probed by Western analysis for SIRT3 (28 kD). For heart extracts, GAPDH (37 kD) was probed for to demonstrate equal protein loading; for mitochondria extracts, Complex I subunit NDUFS1 was used as the loading control (∼75 kD) for mitochondrial samples. Results are from 3 WT and 3 KO mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4549035&req=5

fig01: HFD decreases SIRT3 expression in the heart. (A) WT mice were fed ND or HFD for 16 weeks. Hearts were extracted and Western blot analysis was performed on ventricular lysates. The blot was probed for SIRT3 expression. The blot was stripped and reprobed for GAPDH as a loading control. Bar graph shows quantification of expression. Values are mean ± SEM, n = 6 mice per group. **P < 0.01. (B) Confirmation that KO mice do not express SIRT3. Protein extracts from the ventricles and isolated mitochondria of WT and SIRT3 KO mouse hearts were probed by Western analysis for SIRT3 (28 kD). For heart extracts, GAPDH (37 kD) was probed for to demonstrate equal protein loading; for mitochondria extracts, Complex I subunit NDUFS1 was used as the loading control (∼75 kD) for mitochondrial samples. Results are from 3 WT and 3 KO mice.

Mentions: We first examined SIRT3 expression in the hearts of HFD mice. Feeding mice with HFD for 16 weeks led to a significant reduction in SIRT3 expression in the heart. SIRT3 levels were decreased by 32% in the HFD group compared to the ND group (Fig.1A). We therefore proceeded to compare the consequences of HFD on the heart of WT to SIRT3 KO mice. As shown in Figure1B, we confirmed that SIRT3 KO mice do not express this protein in heart whole tissue or mitochondria extracts.


High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss.

Zeng H, Vaka VR, He X, Booz GW, Chen JX - J. Cell. Mol. Med. (2015)

HFD decreases SIRT3 expression in the heart. (A) WT mice were fed ND or HFD for 16 weeks. Hearts were extracted and Western blot analysis was performed on ventricular lysates. The blot was probed for SIRT3 expression. The blot was stripped and reprobed for GAPDH as a loading control. Bar graph shows quantification of expression. Values are mean ± SEM, n = 6 mice per group. **P < 0.01. (B) Confirmation that KO mice do not express SIRT3. Protein extracts from the ventricles and isolated mitochondria of WT and SIRT3 KO mouse hearts were probed by Western analysis for SIRT3 (28 kD). For heart extracts, GAPDH (37 kD) was probed for to demonstrate equal protein loading; for mitochondria extracts, Complex I subunit NDUFS1 was used as the loading control (∼75 kD) for mitochondrial samples. Results are from 3 WT and 3 KO mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549035&req=5

fig01: HFD decreases SIRT3 expression in the heart. (A) WT mice were fed ND or HFD for 16 weeks. Hearts were extracted and Western blot analysis was performed on ventricular lysates. The blot was probed for SIRT3 expression. The blot was stripped and reprobed for GAPDH as a loading control. Bar graph shows quantification of expression. Values are mean ± SEM, n = 6 mice per group. **P < 0.01. (B) Confirmation that KO mice do not express SIRT3. Protein extracts from the ventricles and isolated mitochondria of WT and SIRT3 KO mouse hearts were probed by Western analysis for SIRT3 (28 kD). For heart extracts, GAPDH (37 kD) was probed for to demonstrate equal protein loading; for mitochondria extracts, Complex I subunit NDUFS1 was used as the loading control (∼75 kD) for mitochondrial samples. Results are from 3 WT and 3 KO mice.
Mentions: We first examined SIRT3 expression in the hearts of HFD mice. Feeding mice with HFD for 16 weeks led to a significant reduction in SIRT3 expression in the heart. SIRT3 levels were decreased by 32% in the HFD group compared to the ND group (Fig.1A). We therefore proceeded to compare the consequences of HFD on the heart of WT to SIRT3 KO mice. As shown in Figure1B, we confirmed that SIRT3 KO mice do not express this protein in heart whole tissue or mitochondria extracts.

Bottom Line: HFD resulted in a significant reduction in SIRT3 expression in the heart.SIRT3 KO mice fed HFD showed greater ROS production and a further reduction in cardiac function compared to SIRT3 KO mice on ND.However, HFD did not further reduce capillary density in SIRT3 KO hearts, implicating SIRT3 loss in HFD-induced capillary rarefaction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, School of Medicine, Jackson, MS, USA.

No MeSH data available.


Related in: MedlinePlus