Limits...
Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.

Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K - J. Cell. Mol. Med. (2015)

Bottom Line: Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro.In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death.In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

No MeSH data available.


Related in: MedlinePlus

Canine CDC engraftment, pro-angiogenic effects, apoptosis measurement. (A) Representative micrographs showing the engraftment of DiI-positive CDCs in the DCM mouse heart. (B) Representative micrographs showing vWF-positive vasculatures. (C) Quantification of vWF-positive vasculatures (n = 9–14 animals per group). (D) Representative micrographs showing apoptosis. (E) Quantitation of cell apoptosis (n = 6). * indicates P < 0.05 when compared to Dox + Saline treatment; scale bars = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4549031&req=5

fig05: Canine CDC engraftment, pro-angiogenic effects, apoptosis measurement. (A) Representative micrographs showing the engraftment of DiI-positive CDCs in the DCM mouse heart. (B) Representative micrographs showing vWF-positive vasculatures. (C) Quantification of vWF-positive vasculatures (n = 9–14 animals per group). (D) Representative micrographs showing apoptosis. (E) Quantitation of cell apoptosis (n = 6). * indicates P < 0.05 when compared to Dox + Saline treatment; scale bars = 50 μm.

Mentions: DiI-positive canine CDCs were detected in the mouse heart (Fig.5A). However, very few engrafted cells acquired mature cardiomyocyte or endothelial phenotypes. Mounting lines of evidence suggest that stem cell transplantation (including CDCs) exerts benefit through paracrine mechanisms, i.e. transplanted cells secrete factors to promote endogenous repair 24. Cardiosphere-derived cell therapy significantly increased vascular density in the DCM heart: vWF-positive vasculatures % of total nuclei in Control-treated hearts versus CDC-treated hearts: 11 ± 1.6 versus 30.4 ± 2.9% (Fig.5B and C). Cardiosphere-derived cell treatment reduced apoptosis in the post-MI hearts (TUNEL-positive cells in Control versus CDC therapy: 1.44 ± 0.23 versus 0.53 ± 0.09%) (Fig.5D and E).


Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.

Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K - J. Cell. Mol. Med. (2015)

Canine CDC engraftment, pro-angiogenic effects, apoptosis measurement. (A) Representative micrographs showing the engraftment of DiI-positive CDCs in the DCM mouse heart. (B) Representative micrographs showing vWF-positive vasculatures. (C) Quantification of vWF-positive vasculatures (n = 9–14 animals per group). (D) Representative micrographs showing apoptosis. (E) Quantitation of cell apoptosis (n = 6). * indicates P < 0.05 when compared to Dox + Saline treatment; scale bars = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549031&req=5

fig05: Canine CDC engraftment, pro-angiogenic effects, apoptosis measurement. (A) Representative micrographs showing the engraftment of DiI-positive CDCs in the DCM mouse heart. (B) Representative micrographs showing vWF-positive vasculatures. (C) Quantification of vWF-positive vasculatures (n = 9–14 animals per group). (D) Representative micrographs showing apoptosis. (E) Quantitation of cell apoptosis (n = 6). * indicates P < 0.05 when compared to Dox + Saline treatment; scale bars = 50 μm.
Mentions: DiI-positive canine CDCs were detected in the mouse heart (Fig.5A). However, very few engrafted cells acquired mature cardiomyocyte or endothelial phenotypes. Mounting lines of evidence suggest that stem cell transplantation (including CDCs) exerts benefit through paracrine mechanisms, i.e. transplanted cells secrete factors to promote endogenous repair 24. Cardiosphere-derived cell therapy significantly increased vascular density in the DCM heart: vWF-positive vasculatures % of total nuclei in Control-treated hearts versus CDC-treated hearts: 11 ± 1.6 versus 30.4 ± 2.9% (Fig.5B and C). Cardiosphere-derived cell treatment reduced apoptosis in the post-MI hearts (TUNEL-positive cells in Control versus CDC therapy: 1.44 ± 0.23 versus 0.53 ± 0.09%) (Fig.5D and E).

Bottom Line: Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro.In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death.In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

No MeSH data available.


Related in: MedlinePlus