Limits...
Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.

Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K - J. Cell. Mol. Med. (2015)

Bottom Line: Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro.In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death.In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

No MeSH data available.


Related in: MedlinePlus

Cardiac function and fibrosis. (A) Schematic diagram showing the design of animal studies. (B) Representative Masson’s Trichrome staining images and quantification of fibrotic area (n = 3) Whole section view (Fig. S3A). (C and D) Change in ejection fraction (EF) and fractional shortening (FS) form baseline measurements (n = 9–11 animals per group); scale bars = 100 μm. *indicates P < 0.05 when compared to the Dox + saline treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4549031&req=5

fig04: Cardiac function and fibrosis. (A) Schematic diagram showing the design of animal studies. (B) Representative Masson’s Trichrome staining images and quantification of fibrotic area (n = 3) Whole section view (Fig. S3A). (C and D) Change in ejection fraction (EF) and fractional shortening (FS) form baseline measurements (n = 9–11 animals per group); scale bars = 100 μm. *indicates P < 0.05 when compared to the Dox + saline treatment.

Mentions: The animal study design is outline in (Fig.4A). Heart sections were stained with Masson’s Trichrome staining kit and analysed for fibrosis. Canine CDC injection significantly reduced fibrosis when compared to Control-treated hearts (Fig.4B, Fig. S3) (fibrosis area % of hearts in Control versus CDC therapy: 13.2 ± 1.4 versus 4.0 ± 0.8%). The bona fide therapeutic effects from stem cell therapy would be the improvement of heart pump functions. Echocardiography was performed at baseline and 7 days after cell or saline injection. Treatment effects (ΔEF% and ΔFS%) were calculated as the change in cardiac functions from the baseline. Saline injection had a negative treatment effects as EFs and FSs deteriorated over the 1 week time (Fig.4C and D, black bars). Cardiosphere-derived cell treatment (Fig.4C and D, red bars) protected cardiac functions from deteriorating because of the doxorubicin. These data suggest that canine CDC therapy mitigates fibrosis and pump function deterioration in DCM.


Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.

Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K - J. Cell. Mol. Med. (2015)

Cardiac function and fibrosis. (A) Schematic diagram showing the design of animal studies. (B) Representative Masson’s Trichrome staining images and quantification of fibrotic area (n = 3) Whole section view (Fig. S3A). (C and D) Change in ejection fraction (EF) and fractional shortening (FS) form baseline measurements (n = 9–11 animals per group); scale bars = 100 μm. *indicates P < 0.05 when compared to the Dox + saline treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549031&req=5

fig04: Cardiac function and fibrosis. (A) Schematic diagram showing the design of animal studies. (B) Representative Masson’s Trichrome staining images and quantification of fibrotic area (n = 3) Whole section view (Fig. S3A). (C and D) Change in ejection fraction (EF) and fractional shortening (FS) form baseline measurements (n = 9–11 animals per group); scale bars = 100 μm. *indicates P < 0.05 when compared to the Dox + saline treatment.
Mentions: The animal study design is outline in (Fig.4A). Heart sections were stained with Masson’s Trichrome staining kit and analysed for fibrosis. Canine CDC injection significantly reduced fibrosis when compared to Control-treated hearts (Fig.4B, Fig. S3) (fibrosis area % of hearts in Control versus CDC therapy: 13.2 ± 1.4 versus 4.0 ± 0.8%). The bona fide therapeutic effects from stem cell therapy would be the improvement of heart pump functions. Echocardiography was performed at baseline and 7 days after cell or saline injection. Treatment effects (ΔEF% and ΔFS%) were calculated as the change in cardiac functions from the baseline. Saline injection had a negative treatment effects as EFs and FSs deteriorated over the 1 week time (Fig.4C and D, black bars). Cardiosphere-derived cell treatment (Fig.4C and D, red bars) protected cardiac functions from deteriorating because of the doxorubicin. These data suggest that canine CDC therapy mitigates fibrosis and pump function deterioration in DCM.

Bottom Line: Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro.In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death.In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

No MeSH data available.


Related in: MedlinePlus