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Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.

Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K - J. Cell. Mol. Med. (2015)

Bottom Line: Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro.In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death.In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

No MeSH data available.


Related in: MedlinePlus

Cardiovascular differentiation of canine CDCs in vitro. (A) Representative fluorescent micrographs showing the expressions of aSA, aSMA and vWF in canine CDCs and MSCs r in three differentiation conditions: CM diff: cardiomyocyte differentiation; SM diff: smooth muscle differentiation; EC diff: endothelial cell differentiation. (B) Percentages of total cells that are positive for specific differentiation markers. * indicates P < 0.05 when compared to cMSC; scale bars = 50 μm.
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fig02: Cardiovascular differentiation of canine CDCs in vitro. (A) Representative fluorescent micrographs showing the expressions of aSA, aSMA and vWF in canine CDCs and MSCs r in three differentiation conditions: CM diff: cardiomyocyte differentiation; SM diff: smooth muscle differentiation; EC diff: endothelial cell differentiation. (B) Percentages of total cells that are positive for specific differentiation markers. * indicates P < 0.05 when compared to cMSC; scale bars = 50 μm.

Mentions: After 12 days into differentiation, around 20% of canine C DCs started to express cardiomyocyte marker α-SA (Fig.2A and B) or cardiac troponin I (Fig. S3) while only ∼5% of canine MSCs expressed α-SA (Fig.2A and B). Instead, canine MSCs were potent in differentiation into smooth muscle cells: ∼90% of canine MSCs express alpha smooth muscle actin. The endothelial differentiation potentials of canine CDCs and MSCs were similar. These assays confirmed the cardiovascular differentiation potential of canine CDCs.


Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.

Hensley MT, de Andrade J, Keene B, Meurs K, Tang J, Wang Z, Caranasos TG, Piedrahita J, Li TS, Cheng K - J. Cell. Mol. Med. (2015)

Cardiovascular differentiation of canine CDCs in vitro. (A) Representative fluorescent micrographs showing the expressions of aSA, aSMA and vWF in canine CDCs and MSCs r in three differentiation conditions: CM diff: cardiomyocyte differentiation; SM diff: smooth muscle differentiation; EC diff: endothelial cell differentiation. (B) Percentages of total cells that are positive for specific differentiation markers. * indicates P < 0.05 when compared to cMSC; scale bars = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549031&req=5

fig02: Cardiovascular differentiation of canine CDCs in vitro. (A) Representative fluorescent micrographs showing the expressions of aSA, aSMA and vWF in canine CDCs and MSCs r in three differentiation conditions: CM diff: cardiomyocyte differentiation; SM diff: smooth muscle differentiation; EC diff: endothelial cell differentiation. (B) Percentages of total cells that are positive for specific differentiation markers. * indicates P < 0.05 when compared to cMSC; scale bars = 50 μm.
Mentions: After 12 days into differentiation, around 20% of canine C DCs started to express cardiomyocyte marker α-SA (Fig.2A and B) or cardiac troponin I (Fig. S3) while only ∼5% of canine MSCs expressed α-SA (Fig.2A and B). Instead, canine MSCs were potent in differentiation into smooth muscle cells: ∼90% of canine MSCs express alpha smooth muscle actin. The endothelial differentiation potentials of canine CDCs and MSCs were similar. These assays confirmed the cardiovascular differentiation potential of canine CDCs.

Bottom Line: Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro.In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death.In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

No MeSH data available.


Related in: MedlinePlus