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The secretome of myocardial telocytes modulates the activity of cardiac stem cells.

Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM - J. Cell. Mol. Med. (2015)

Bottom Line: We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate.We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs.The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry-Proteomics Department, Victor Babeş National Institute of Pathology, Bucharest, Romania.

No MeSH data available.


Related in: MedlinePlus

Luminex-xMAP detection of secreted cyto- and chemokines in the cell culture supernatants of rat myocardial TC mono-, CSC mono- and TC-CSC co-cultures: (A) IL-6, (B) MIP-2, (C) MIP-1α, (D) MCP-1. The results were normalized to cell counts. P < 0.05, n = 3.
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fig06: Luminex-xMAP detection of secreted cyto- and chemokines in the cell culture supernatants of rat myocardial TC mono-, CSC mono- and TC-CSC co-cultures: (A) IL-6, (B) MIP-2, (C) MIP-1α, (D) MCP-1. The results were normalized to cell counts. P < 0.05, n = 3.

Mentions: The rat secretome contained significantly more protein than the CSC secretome. IL-6 was significantly expressed in the rat secretome at levels similar to those in the mouse TC secretome, i.e., TCs expressed a fivefold higher level than CSCs (Fig.6A). This concentration remained high and similar to the TC secretome level in the TC-CSC co-culture supernatant.


The secretome of myocardial telocytes modulates the activity of cardiac stem cells.

Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM - J. Cell. Mol. Med. (2015)

Luminex-xMAP detection of secreted cyto- and chemokines in the cell culture supernatants of rat myocardial TC mono-, CSC mono- and TC-CSC co-cultures: (A) IL-6, (B) MIP-2, (C) MIP-1α, (D) MCP-1. The results were normalized to cell counts. P < 0.05, n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549029&req=5

fig06: Luminex-xMAP detection of secreted cyto- and chemokines in the cell culture supernatants of rat myocardial TC mono-, CSC mono- and TC-CSC co-cultures: (A) IL-6, (B) MIP-2, (C) MIP-1α, (D) MCP-1. The results were normalized to cell counts. P < 0.05, n = 3.
Mentions: The rat secretome contained significantly more protein than the CSC secretome. IL-6 was significantly expressed in the rat secretome at levels similar to those in the mouse TC secretome, i.e., TCs expressed a fivefold higher level than CSCs (Fig.6A). This concentration remained high and similar to the TC secretome level in the TC-CSC co-culture supernatant.

Bottom Line: We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate.We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs.The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry-Proteomics Department, Victor Babeş National Institute of Pathology, Bucharest, Romania.

No MeSH data available.


Related in: MedlinePlus