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The secretome of myocardial telocytes modulates the activity of cardiac stem cells.

Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM - J. Cell. Mol. Med. (2015)

Bottom Line: We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate.We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs.The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry-Proteomics Department, Victor Babeş National Institute of Pathology, Bucharest, Romania.

No MeSH data available.


Related in: MedlinePlus

The levels of IL 6 cytokine in the serum-free culture supernatants of mouse myocardial TCs compared to 3T3 fibroblasts as determined by xMAP technology. The data are expressed as the mean ± SE from three independent experiments and were analysed with Student’s t-test. TC1 – first passage mouse TCs, TC2 – second passage mouse TCs, TC3 – third passage mouse TCs, FB: fibroblasts.
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fig03: The levels of IL 6 cytokine in the serum-free culture supernatants of mouse myocardial TCs compared to 3T3 fibroblasts as determined by xMAP technology. The data are expressed as the mean ± SE from three independent experiments and were analysed with Student’s t-test. TC1 – first passage mouse TCs, TC2 – second passage mouse TCs, TC3 – third passage mouse TCs, FB: fibroblasts.

Mentions: To precisely identify the detected proteins, we further used xMAP analysis to examine the protein composition of mouse TCs supernatants. We identified IL-6, VEGF and MIP-2, but no other inflammatory cytokines or mediators, such as TNF-α, IL-1β, IL-2 or IL-4, were detected (Figs3 and 4). Table1 shows the synthesis of the secretome of mouse TCs with additional data that were extracted from the UniProtKB database 67.


The secretome of myocardial telocytes modulates the activity of cardiac stem cells.

Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM - J. Cell. Mol. Med. (2015)

The levels of IL 6 cytokine in the serum-free culture supernatants of mouse myocardial TCs compared to 3T3 fibroblasts as determined by xMAP technology. The data are expressed as the mean ± SE from three independent experiments and were analysed with Student’s t-test. TC1 – first passage mouse TCs, TC2 – second passage mouse TCs, TC3 – third passage mouse TCs, FB: fibroblasts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549029&req=5

fig03: The levels of IL 6 cytokine in the serum-free culture supernatants of mouse myocardial TCs compared to 3T3 fibroblasts as determined by xMAP technology. The data are expressed as the mean ± SE from three independent experiments and were analysed with Student’s t-test. TC1 – first passage mouse TCs, TC2 – second passage mouse TCs, TC3 – third passage mouse TCs, FB: fibroblasts.
Mentions: To precisely identify the detected proteins, we further used xMAP analysis to examine the protein composition of mouse TCs supernatants. We identified IL-6, VEGF and MIP-2, but no other inflammatory cytokines or mediators, such as TNF-α, IL-1β, IL-2 or IL-4, were detected (Figs3 and 4). Table1 shows the synthesis of the secretome of mouse TCs with additional data that were extracted from the UniProtKB database 67.

Bottom Line: We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate.We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs.The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry-Proteomics Department, Victor Babeş National Institute of Pathology, Bucharest, Romania.

No MeSH data available.


Related in: MedlinePlus