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The secretome of myocardial telocytes modulates the activity of cardiac stem cells.

Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM - J. Cell. Mol. Med. (2015)

Bottom Line: We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate.We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs.The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry-Proteomics Department, Victor Babeş National Institute of Pathology, Bucharest, Romania.

No MeSH data available.


Related in: MedlinePlus

Protein profiling analysis of TC and FB supernatants by surface-enhanced laser desorption and ionization mass spectrometry. Characteristic proteins that are secreted by TCs were identified at m/z values of ∼11.7 kD, from 13.8 to 14.8, and at 22.5 kD. The peaks suggest the presence of MIP-1α, MIP-2 dimer, MCP-1 and VEGF. m/z range: 0–25,000 D, CM10 chips, SPA matrix. Control – cell culture medium, TC-P1 – first passage, TC-P2 – second passage, TC-P3 – third passage, FB – fibroblasts.
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fig02: Protein profiling analysis of TC and FB supernatants by surface-enhanced laser desorption and ionization mass spectrometry. Characteristic proteins that are secreted by TCs were identified at m/z values of ∼11.7 kD, from 13.8 to 14.8, and at 22.5 kD. The peaks suggest the presence of MIP-1α, MIP-2 dimer, MCP-1 and VEGF. m/z range: 0–25,000 D, CM10 chips, SPA matrix. Control – cell culture medium, TC-P1 – first passage, TC-P2 – second passage, TC-P3 – third passage, FB – fibroblasts.

Mentions: SELDI-TOF MS protein profiling was used to analyse and compare several differentially expressed peptide and protein peaks that were identified in the mouse TC culture supernatants, the culture medium and the 3T3 fibroblast culture supernatants. Additionally, several differentially expressed peptide and protein peaks in TCs supernatants (maintained in serum-free medium) were demonstrated by MS. Mass peaks between 1.5 and 25 kD were analysed and the most significant peaks were estimated at m/z values of 11.7 estimated to be most significant. Significant peaks were identified between 13.8 and 14.8 and at 22.5 kD (Fig.2). Using the UniProtKB database 67, the 22.5 kD protein was assigned to the classical family of cytokines/growth factors, and this protein most likely is VEGF or IL-6. Other protein peaks caught our attention, such as those that possibly matched MIP-1α, MIP-2 dimer and MCP-1.


The secretome of myocardial telocytes modulates the activity of cardiac stem cells.

Albulescu R, Tanase C, Codrici E, Popescu DI, Cretoiu SM, Popescu LM - J. Cell. Mol. Med. (2015)

Protein profiling analysis of TC and FB supernatants by surface-enhanced laser desorption and ionization mass spectrometry. Characteristic proteins that are secreted by TCs were identified at m/z values of ∼11.7 kD, from 13.8 to 14.8, and at 22.5 kD. The peaks suggest the presence of MIP-1α, MIP-2 dimer, MCP-1 and VEGF. m/z range: 0–25,000 D, CM10 chips, SPA matrix. Control – cell culture medium, TC-P1 – first passage, TC-P2 – second passage, TC-P3 – third passage, FB – fibroblasts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4549029&req=5

fig02: Protein profiling analysis of TC and FB supernatants by surface-enhanced laser desorption and ionization mass spectrometry. Characteristic proteins that are secreted by TCs were identified at m/z values of ∼11.7 kD, from 13.8 to 14.8, and at 22.5 kD. The peaks suggest the presence of MIP-1α, MIP-2 dimer, MCP-1 and VEGF. m/z range: 0–25,000 D, CM10 chips, SPA matrix. Control – cell culture medium, TC-P1 – first passage, TC-P2 – second passage, TC-P3 – third passage, FB – fibroblasts.
Mentions: SELDI-TOF MS protein profiling was used to analyse and compare several differentially expressed peptide and protein peaks that were identified in the mouse TC culture supernatants, the culture medium and the 3T3 fibroblast culture supernatants. Additionally, several differentially expressed peptide and protein peaks in TCs supernatants (maintained in serum-free medium) were demonstrated by MS. Mass peaks between 1.5 and 25 kD were analysed and the most significant peaks were estimated at m/z values of 11.7 estimated to be most significant. Significant peaks were identified between 13.8 and 14.8 and at 22.5 kD (Fig.2). Using the UniProtKB database 67, the 22.5 kD protein was assigned to the classical family of cytokines/growth factors, and this protein most likely is VEGF or IL-6. Other protein peaks caught our attention, such as those that possibly matched MIP-1α, MIP-2 dimer and MCP-1.

Bottom Line: We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate.We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs.The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry-Proteomics Department, Victor Babeş National Institute of Pathology, Bucharest, Romania.

No MeSH data available.


Related in: MedlinePlus