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The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.


Proliferation of cells treated with Brazilian red propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Brazilian red propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars
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Fig6: Proliferation of cells treated with Brazilian red propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Brazilian red propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars

Mentions: Brazilian red propolis, the 100 μg/mL concentration showed the highest average cell count at 48 h (Fig. 6a). However, it only had a marginally higher number of viable cells when compared to the DMSO control. The 10 and 250 μg/mL concentrations indicated a slight increase in proliferation activity from 24 to 48 h, although this was still lower than that shown by the control. The 500 and 1000 μg/mL concentrations had a constant decrease in cell count throughout the entire duration. All the concentrations apart from the 100 μg/mL concentration resulted in less proliferation activity compared to the control. However, the differences between the mean number of cells proliferated in the presence of the treatments and the control were not statistically significant (p > 0.05). Nevertheless, an increasing trend between concentration and cell count was observed until 100 μg/mL, and then a decreasing trend was observed as the concentration increased (Fig. 6b).Fig. 6


The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Proliferation of cells treated with Brazilian red propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Brazilian red propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Fig6: Proliferation of cells treated with Brazilian red propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Brazilian red propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars
Mentions: Brazilian red propolis, the 100 μg/mL concentration showed the highest average cell count at 48 h (Fig. 6a). However, it only had a marginally higher number of viable cells when compared to the DMSO control. The 10 and 250 μg/mL concentrations indicated a slight increase in proliferation activity from 24 to 48 h, although this was still lower than that shown by the control. The 500 and 1000 μg/mL concentrations had a constant decrease in cell count throughout the entire duration. All the concentrations apart from the 100 μg/mL concentration resulted in less proliferation activity compared to the control. However, the differences between the mean number of cells proliferated in the presence of the treatments and the control were not statistically significant (p > 0.05). Nevertheless, an increasing trend between concentration and cell count was observed until 100 μg/mL, and then a decreasing trend was observed as the concentration increased (Fig. 6b).Fig. 6

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.