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The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.


Proliferation of cells treated with Malaysian propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Malaysian propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Malaysian propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars
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Fig5: Proliferation of cells treated with Malaysian propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Malaysian propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Malaysian propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars

Mentions: In the experiment using Malaysian propolis, the 500 μg/mL concentration showed the highest increase in cell count after 48 h. This was followed by 250 and 1000 μg/mL concentrations respectively. The DMSO control and 100 μg/mL concentrations indicated marginally higher proliferation activity compared to the control, while 10 and 1 μg/mL concentrations had lower proliferation compared to the control. However, there were no statistically significant differences (p > 0.05) between the cell counts of all the treatment concentrations when compared to the control (Fig. 5a). An increase in the number of cells was observed as the concentration increased from 1 to 500 μg/mL, and then a decrease from 500 to 1000 μg/mL. This concentration-dependent trend by which Malaysian propolis exerts its effect on the proliferation of fibroblast cells can be seen in Fig. 5b.Fig. 5


The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Proliferation of cells treated with Malaysian propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Malaysian propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Malaysian propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4549008&req=5

Fig5: Proliferation of cells treated with Malaysian propolis. a Line graph showing proliferation of treated and untreated cells using different concentrations of Malaysian propolis at various time points. Values are expressed as mean number of cells ± standard error as indicated by the error bars. There was no significant difference between the mean number of cells of the control and treatments. b Polynomial trend line showing the concentration-dependent trend of the proliferation of cells treated with Malaysian propolis at 48 h. Values are expressed as mean number of cells ± standard error as indicated by the error bars
Mentions: In the experiment using Malaysian propolis, the 500 μg/mL concentration showed the highest increase in cell count after 48 h. This was followed by 250 and 1000 μg/mL concentrations respectively. The DMSO control and 100 μg/mL concentrations indicated marginally higher proliferation activity compared to the control, while 10 and 1 μg/mL concentrations had lower proliferation compared to the control. However, there were no statistically significant differences (p > 0.05) between the cell counts of all the treatment concentrations when compared to the control (Fig. 5a). An increase in the number of cells was observed as the concentration increased from 1 to 500 μg/mL, and then a decrease from 500 to 1000 μg/mL. This concentration-dependent trend by which Malaysian propolis exerts its effect on the proliferation of fibroblast cells can be seen in Fig. 5b.Fig. 5

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.