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The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.


Related in: MedlinePlus

Migration rates of cells treated with Brazilian red propolis. a Line graph showing migration rates of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean migration rate ± standard error as indicated by the error bars. Significance: *p-value < 0.05 treatment vs control. b Polynomial trend line showing the concentration-dependent trend of the migration rate of cells treated with Brazilian red propolis over 24 h. Values are expressed as mean migration rate ± standard error as indicated by the error bars
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Fig4: Migration rates of cells treated with Brazilian red propolis. a Line graph showing migration rates of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean migration rate ± standard error as indicated by the error bars. Significance: *p-value < 0.05 treatment vs control. b Polynomial trend line showing the concentration-dependent trend of the migration rate of cells treated with Brazilian red propolis over 24 h. Values are expressed as mean migration rate ± standard error as indicated by the error bars

Mentions: In the first 12 h, the control showed the fastest migration rate compared to all treatment concentrations (Fig. 4a). However, only the 250 μg/mL (p = 0.001), 500 μg/mL (p = 0.01) and 1000 μg/mL (p = 0.000) concentrations showed a statistically slower migration rate than control. Over the 24–hour period, all treatment concentrations had a statistically significant slower average migration rate compared to that of the control (p < 0.05), with the exception of the 10 μg/mL concentration. The cells treated with 10 μg/mL concentration indicated a slightly faster rate of migration than the control, though not statistically significant (p > 0.05). Brazilian red propolis appeared to follow a concentration-dependent trend in its effect on fibroblast cell migration over the 24-hour period. The migration rate increased with an increase in concentration from 1 to 10 μg/mL, and then showed an inverse correlation as the migration rate declined as the concentration increased from 10 μg/mL (Fig. 4b). At 12 h cell migration rate in the presence of DMSO was higher than Brazilian red propolis at 250, 500 and 1000 μg/mL concentrations that indicates a potential cytotoxicity of Brazilian propolis more than 250 μg/mL concentrations (Fig. 4a).Fig. 4


The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Migration rates of cells treated with Brazilian red propolis. a Line graph showing migration rates of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean migration rate ± standard error as indicated by the error bars. Significance: *p-value < 0.05 treatment vs control. b Polynomial trend line showing the concentration-dependent trend of the migration rate of cells treated with Brazilian red propolis over 24 h. Values are expressed as mean migration rate ± standard error as indicated by the error bars
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4549008&req=5

Fig4: Migration rates of cells treated with Brazilian red propolis. a Line graph showing migration rates of treated and untreated cells using different concentrations of Brazilian red propolis at various time points. Values are expressed as mean migration rate ± standard error as indicated by the error bars. Significance: *p-value < 0.05 treatment vs control. b Polynomial trend line showing the concentration-dependent trend of the migration rate of cells treated with Brazilian red propolis over 24 h. Values are expressed as mean migration rate ± standard error as indicated by the error bars
Mentions: In the first 12 h, the control showed the fastest migration rate compared to all treatment concentrations (Fig. 4a). However, only the 250 μg/mL (p = 0.001), 500 μg/mL (p = 0.01) and 1000 μg/mL (p = 0.000) concentrations showed a statistically slower migration rate than control. Over the 24–hour period, all treatment concentrations had a statistically significant slower average migration rate compared to that of the control (p < 0.05), with the exception of the 10 μg/mL concentration. The cells treated with 10 μg/mL concentration indicated a slightly faster rate of migration than the control, though not statistically significant (p > 0.05). Brazilian red propolis appeared to follow a concentration-dependent trend in its effect on fibroblast cell migration over the 24-hour period. The migration rate increased with an increase in concentration from 1 to 10 μg/mL, and then showed an inverse correlation as the migration rate declined as the concentration increased from 10 μg/mL (Fig. 4b). At 12 h cell migration rate in the presence of DMSO was higher than Brazilian red propolis at 250, 500 and 1000 μg/mL concentrations that indicates a potential cytotoxicity of Brazilian propolis more than 250 μg/mL concentrations (Fig. 4a).Fig. 4

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.


Related in: MedlinePlus